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Association of Two Polymorphisms in H2B.W Gene with Azoospermia and Severe Oligozoospermia in An Iranian Population
BACKGROUND: During spermatogenesis, the H2B family, member W (H2B.W) gene, en- codes a testis specific histone that is co-localized with telomeric sequences and has the potential role to mediate the sperm-specific chromatin remodeling. Previously H2B.W genetic variants were reported to be involved i...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4518489/ https://www.ncbi.nlm.nih.gov/pubmed/26246879 |
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author | Haji Ebrahim Zargar, Haleh Mohseni Meybodi, Anahita Sabbaghian, Marjan Shahhoseini, Maryam Asadpor, Ummulbanin Sadighi Gilani, Mohammad Ali Chehrazi, Mohammad Farhangniya, Mansoureh Shahzadeh Fazeli, Seyed Abolhassan |
author_facet | Haji Ebrahim Zargar, Haleh Mohseni Meybodi, Anahita Sabbaghian, Marjan Shahhoseini, Maryam Asadpor, Ummulbanin Sadighi Gilani, Mohammad Ali Chehrazi, Mohammad Farhangniya, Mansoureh Shahzadeh Fazeli, Seyed Abolhassan |
author_sort | Haji Ebrahim Zargar, Haleh |
collection | PubMed |
description | BACKGROUND: During spermatogenesis, the H2B family, member W (H2B.W) gene, en- codes a testis specific histone that is co-localized with telomeric sequences and has the potential role to mediate the sperm-specific chromatin remodeling. Previously H2B.W genetic variants were reported to be involved in susceptibility to spermatogenesis im- pairment. In the present study, two single nucleotide polymorphisms (SNPs) in 5΄UTR and exon 1 of H2B.W gene were examined to investigate possible association of these polymorphisms with male infertility in Iranian population. MATERIALS AND METHODS: This case control study was conducted in Royan institute during four-year period (2010–2013). Genetic alteration of two SNPs loci, −9C>T and 368A>G, in H2B.W gene were indicated in 92 infertile men who were divided into two main groups includ- ing azoospermia (n=46) and sever oligozoospermia (n=46), while there was 60 fertile men as control group. Azoosperima was also divided into three sub-groups including sertoli cell only syndrome (SCOS, n=21), complete maturation arrest (CMA, n=17) and hypo spermatogenesis (n=8) according to testicular biopsy. For analysis, polymerase chain reaction-restriction frag- ment length polymorphism (PCR-RFLP) technique was applied. RESULTS: The frequency of allele −9T was significantly higher in CMA group than in patients with SCOS (P<0.05). The haplotype TA (corresponding to simultaneous occur- rence of −9T and 368A) compared with haplotype CA (corresponding to simultaneous occurrence of −9C and 368A) in patients suffering from CMA significantly increased, compared with patients had SCOS (P<0.05). However, statistical studies indicated that in general, the distribution frequencies of −9C>T and 368A>G had no significant difference between the infertile groups and control (P=0.859 and P=0.812, respectively). CONCLUSION: This investigation showed that SNP −9C>T might be contribute to CMA in azoo- spermic patients and SNP 368A>G had no correlation with male infertility in Iranian population. |
format | Online Article Text |
id | pubmed-4518489 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-45184892015-08-05 Association of Two Polymorphisms in H2B.W Gene with Azoospermia and Severe Oligozoospermia in An Iranian Population Haji Ebrahim Zargar, Haleh Mohseni Meybodi, Anahita Sabbaghian, Marjan Shahhoseini, Maryam Asadpor, Ummulbanin Sadighi Gilani, Mohammad Ali Chehrazi, Mohammad Farhangniya, Mansoureh Shahzadeh Fazeli, Seyed Abolhassan Int J Fertil Steril Original Article BACKGROUND: During spermatogenesis, the H2B family, member W (H2B.W) gene, en- codes a testis specific histone that is co-localized with telomeric sequences and has the potential role to mediate the sperm-specific chromatin remodeling. Previously H2B.W genetic variants were reported to be involved in susceptibility to spermatogenesis im- pairment. In the present study, two single nucleotide polymorphisms (SNPs) in 5΄UTR and exon 1 of H2B.W gene were examined to investigate possible association of these polymorphisms with male infertility in Iranian population. MATERIALS AND METHODS: This case control study was conducted in Royan institute during four-year period (2010–2013). Genetic alteration of two SNPs loci, −9C>T and 368A>G, in H2B.W gene were indicated in 92 infertile men who were divided into two main groups includ- ing azoospermia (n=46) and sever oligozoospermia (n=46), while there was 60 fertile men as control group. Azoosperima was also divided into three sub-groups including sertoli cell only syndrome (SCOS, n=21), complete maturation arrest (CMA, n=17) and hypo spermatogenesis (n=8) according to testicular biopsy. For analysis, polymerase chain reaction-restriction frag- ment length polymorphism (PCR-RFLP) technique was applied. RESULTS: The frequency of allele −9T was significantly higher in CMA group than in patients with SCOS (P<0.05). The haplotype TA (corresponding to simultaneous occur- rence of −9T and 368A) compared with haplotype CA (corresponding to simultaneous occurrence of −9C and 368A) in patients suffering from CMA significantly increased, compared with patients had SCOS (P<0.05). However, statistical studies indicated that in general, the distribution frequencies of −9C>T and 368A>G had no significant difference between the infertile groups and control (P=0.859 and P=0.812, respectively). CONCLUSION: This investigation showed that SNP −9C>T might be contribute to CMA in azoo- spermic patients and SNP 368A>G had no correlation with male infertility in Iranian population. Royan Institute 2015 2015-07-27 /pmc/articles/PMC4518489/ /pubmed/26246879 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Haji Ebrahim Zargar, Haleh Mohseni Meybodi, Anahita Sabbaghian, Marjan Shahhoseini, Maryam Asadpor, Ummulbanin Sadighi Gilani, Mohammad Ali Chehrazi, Mohammad Farhangniya, Mansoureh Shahzadeh Fazeli, Seyed Abolhassan Association of Two Polymorphisms in H2B.W Gene with Azoospermia and Severe Oligozoospermia in An Iranian Population |
title | Association of Two Polymorphisms in H2B.W Gene with
Azoospermia and Severe Oligozoospermia in
An Iranian Population |
title_full | Association of Two Polymorphisms in H2B.W Gene with
Azoospermia and Severe Oligozoospermia in
An Iranian Population |
title_fullStr | Association of Two Polymorphisms in H2B.W Gene with
Azoospermia and Severe Oligozoospermia in
An Iranian Population |
title_full_unstemmed | Association of Two Polymorphisms in H2B.W Gene with
Azoospermia and Severe Oligozoospermia in
An Iranian Population |
title_short | Association of Two Polymorphisms in H2B.W Gene with
Azoospermia and Severe Oligozoospermia in
An Iranian Population |
title_sort | association of two polymorphisms in h2b.w gene with
azoospermia and severe oligozoospermia in
an iranian population |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4518489/ https://www.ncbi.nlm.nih.gov/pubmed/26246879 |
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