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Effect of hypertonic saline treatment on the inflammatory response after hydrochloric acid-induced lung injury in pigs

OBJECTIVES: Hypertonic saline has been proposed to modulate the inflammatory cascade in certain experimental conditions, including pulmonary inflammation caused by inhaled gastric contents. The present study aimed to assess the potential anti-inflammatory effects of administering a single intravenou...

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Autores principales: Holms, Carla Augusto, Otsuki, Denise Aya, Kahvegian, Marcia, Massoco, Cristina Oliveira, Fantoni, Denise Tabacchi, Gutierrez, Paulo Sampaio, Junior, Jose Otavio Costa Auler
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4518820/
https://www.ncbi.nlm.nih.gov/pubmed/26247671
http://dx.doi.org/10.6061/clinics/2015(08)08
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author Holms, Carla Augusto
Otsuki, Denise Aya
Kahvegian, Marcia
Massoco, Cristina Oliveira
Fantoni, Denise Tabacchi
Gutierrez, Paulo Sampaio
Junior, Jose Otavio Costa Auler
author_facet Holms, Carla Augusto
Otsuki, Denise Aya
Kahvegian, Marcia
Massoco, Cristina Oliveira
Fantoni, Denise Tabacchi
Gutierrez, Paulo Sampaio
Junior, Jose Otavio Costa Auler
author_sort Holms, Carla Augusto
collection PubMed
description OBJECTIVES: Hypertonic saline has been proposed to modulate the inflammatory cascade in certain experimental conditions, including pulmonary inflammation caused by inhaled gastric contents. The present study aimed to assess the potential anti-inflammatory effects of administering a single intravenous dose of 7.5% hypertonic saline in an experimental model of acute lung injury induced by hydrochloric acid. METHODS: Thirty-two pigs were anesthetized and randomly allocated into the following four groups: Sham, which received anesthesia and were observed; HS, which received intravenous 7.5% hypertonic saline solution (4 ml/kg); acute lung injury, which were subjected to acute lung injury with intratracheal hydrochloric acid; and acute lung injury + hypertonic saline, which were subjected to acute lung injury with hydrochloric acid and treated with hypertonic saline. Hemodynamic and ventilatory parameters were recorded over four hours. Subsequently, bronchoalveolar lavage samples were collected at the end of the observation period to measure cytokine levels using an oxidative burst analysis, and lung tissue was collected for a histological analysis. RESULTS: Hydrochloric acid instillation caused marked changes in respiratory mechanics as well as blood gas and lung parenchyma parameters. Despite the absence of a significant difference between the acute lung injury and acute lung injury + hypertonic saline groups, the acute lung injury animals presented higher neutrophil and tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-8 levels in the bronchoalveolar lavage analysis. The histopathological analysis revealed pulmonary edema, congestion and alveolar collapse in both groups; however, the differences between groups were not significant. Despite the lower cytokine and neutrophil levels observed in the acute lung injury + hypertonic saline group, significant differences were not observed among the treated and non-treated groups. CONCLUSIONS: Hypertonic saline infusion after intratracheal hydrochloric acid instillation does not have an effect on inflammatory biomarkers or respiratory gas exchange.
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spelling pubmed-45188202015-08-17 Effect of hypertonic saline treatment on the inflammatory response after hydrochloric acid-induced lung injury in pigs Holms, Carla Augusto Otsuki, Denise Aya Kahvegian, Marcia Massoco, Cristina Oliveira Fantoni, Denise Tabacchi Gutierrez, Paulo Sampaio Junior, Jose Otavio Costa Auler Clinics (Sao Paulo) Basic Research OBJECTIVES: Hypertonic saline has been proposed to modulate the inflammatory cascade in certain experimental conditions, including pulmonary inflammation caused by inhaled gastric contents. The present study aimed to assess the potential anti-inflammatory effects of administering a single intravenous dose of 7.5% hypertonic saline in an experimental model of acute lung injury induced by hydrochloric acid. METHODS: Thirty-two pigs were anesthetized and randomly allocated into the following four groups: Sham, which received anesthesia and were observed; HS, which received intravenous 7.5% hypertonic saline solution (4 ml/kg); acute lung injury, which were subjected to acute lung injury with intratracheal hydrochloric acid; and acute lung injury + hypertonic saline, which were subjected to acute lung injury with hydrochloric acid and treated with hypertonic saline. Hemodynamic and ventilatory parameters were recorded over four hours. Subsequently, bronchoalveolar lavage samples were collected at the end of the observation period to measure cytokine levels using an oxidative burst analysis, and lung tissue was collected for a histological analysis. RESULTS: Hydrochloric acid instillation caused marked changes in respiratory mechanics as well as blood gas and lung parenchyma parameters. Despite the absence of a significant difference between the acute lung injury and acute lung injury + hypertonic saline groups, the acute lung injury animals presented higher neutrophil and tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-8 levels in the bronchoalveolar lavage analysis. The histopathological analysis revealed pulmonary edema, congestion and alveolar collapse in both groups; however, the differences between groups were not significant. Despite the lower cytokine and neutrophil levels observed in the acute lung injury + hypertonic saline group, significant differences were not observed among the treated and non-treated groups. CONCLUSIONS: Hypertonic saline infusion after intratracheal hydrochloric acid instillation does not have an effect on inflammatory biomarkers or respiratory gas exchange. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2015-08 2015-08 /pmc/articles/PMC4518820/ /pubmed/26247671 http://dx.doi.org/10.6061/clinics/2015(08)08 Text en Copyright © 2015 Clinics http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Holms, Carla Augusto
Otsuki, Denise Aya
Kahvegian, Marcia
Massoco, Cristina Oliveira
Fantoni, Denise Tabacchi
Gutierrez, Paulo Sampaio
Junior, Jose Otavio Costa Auler
Effect of hypertonic saline treatment on the inflammatory response after hydrochloric acid-induced lung injury in pigs
title Effect of hypertonic saline treatment on the inflammatory response after hydrochloric acid-induced lung injury in pigs
title_full Effect of hypertonic saline treatment on the inflammatory response after hydrochloric acid-induced lung injury in pigs
title_fullStr Effect of hypertonic saline treatment on the inflammatory response after hydrochloric acid-induced lung injury in pigs
title_full_unstemmed Effect of hypertonic saline treatment on the inflammatory response after hydrochloric acid-induced lung injury in pigs
title_short Effect of hypertonic saline treatment on the inflammatory response after hydrochloric acid-induced lung injury in pigs
title_sort effect of hypertonic saline treatment on the inflammatory response after hydrochloric acid-induced lung injury in pigs
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4518820/
https://www.ncbi.nlm.nih.gov/pubmed/26247671
http://dx.doi.org/10.6061/clinics/2015(08)08
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