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Gender and Obesity Specific MicroRNA Expression in Adipose Tissue from Lean and Obese Pigs

Obesity is a complex condition that increases the risk of life threatening diseases such as cardiovascular disease and diabetes. Studying the gene regulation of obesity is important for understanding the molecular mechanisms behind the obesity derived diseases and may lead to better intervention and...

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Autores principales: Mentzel, Caroline M. Junker, Anthon, Christian, Jacobsen, Mette J., Karlskov-Mortensen, Peter, Bruun, Camilla S., Jørgensen, Claus B., Gorodkin, Jan, Cirera, Susanna, Fredholm, Merete
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519260/
https://www.ncbi.nlm.nih.gov/pubmed/26222688
http://dx.doi.org/10.1371/journal.pone.0131650
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author Mentzel, Caroline M. Junker
Anthon, Christian
Jacobsen, Mette J.
Karlskov-Mortensen, Peter
Bruun, Camilla S.
Jørgensen, Claus B.
Gorodkin, Jan
Cirera, Susanna
Fredholm, Merete
author_facet Mentzel, Caroline M. Junker
Anthon, Christian
Jacobsen, Mette J.
Karlskov-Mortensen, Peter
Bruun, Camilla S.
Jørgensen, Claus B.
Gorodkin, Jan
Cirera, Susanna
Fredholm, Merete
author_sort Mentzel, Caroline M. Junker
collection PubMed
description Obesity is a complex condition that increases the risk of life threatening diseases such as cardiovascular disease and diabetes. Studying the gene regulation of obesity is important for understanding the molecular mechanisms behind the obesity derived diseases and may lead to better intervention and treatment plans. MicroRNAs (miRNAs) are short non-coding RNAs regulating target mRNA by binding to their 3’UTR. They are involved in numerous biological processes and diseases, including obesity. In this study we use a mixed breed pig model designed for obesity studies to investigate differentially expressed miRNAs in subcutaneous adipose tissue by RNA sequencing (RNAseq). Both male and female pigs are included to explore gender differences. The RNAseq study shows that the most highly expressed miRNAs are in accordance with comparable studies in pigs and humans. A total of six miRNAs are differentially expressed in subcutaneous adipose tissue between the lean and obese group of pigs, and in addition gender specific significant differential expression is observed for a number of miRNAs. The differentially expressed miRNAs have been verified using qPCR. The results of these studies in general confirm the trends found by RNAseq. Mir-9 and mir-124a are significantly differentially expressed with large fold changes in subcutaneous adipose tissue between lean and obese pigs. Mir-9 is more highly expressed in the obese pigs with a fold change of 10 and a p-value < 0.001. Mir-124a is more highly expressed in the obese pigs with a fold change of 114 and a p-value < 0.001. In addition, mir-124a is significantly higher expressed in abdominal adipose tissue in male pigs with a fold change of 119 and a p-value < 0.05. Both miRNAs are also significantly higher expressed in the liver of obese male pigs where mir-124a has a fold change of 12 and mir-9 has a fold change of 1.6, both with p-values < 0.05.
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spelling pubmed-45192602015-07-31 Gender and Obesity Specific MicroRNA Expression in Adipose Tissue from Lean and Obese Pigs Mentzel, Caroline M. Junker Anthon, Christian Jacobsen, Mette J. Karlskov-Mortensen, Peter Bruun, Camilla S. Jørgensen, Claus B. Gorodkin, Jan Cirera, Susanna Fredholm, Merete PLoS One Research Article Obesity is a complex condition that increases the risk of life threatening diseases such as cardiovascular disease and diabetes. Studying the gene regulation of obesity is important for understanding the molecular mechanisms behind the obesity derived diseases and may lead to better intervention and treatment plans. MicroRNAs (miRNAs) are short non-coding RNAs regulating target mRNA by binding to their 3’UTR. They are involved in numerous biological processes and diseases, including obesity. In this study we use a mixed breed pig model designed for obesity studies to investigate differentially expressed miRNAs in subcutaneous adipose tissue by RNA sequencing (RNAseq). Both male and female pigs are included to explore gender differences. The RNAseq study shows that the most highly expressed miRNAs are in accordance with comparable studies in pigs and humans. A total of six miRNAs are differentially expressed in subcutaneous adipose tissue between the lean and obese group of pigs, and in addition gender specific significant differential expression is observed for a number of miRNAs. The differentially expressed miRNAs have been verified using qPCR. The results of these studies in general confirm the trends found by RNAseq. Mir-9 and mir-124a are significantly differentially expressed with large fold changes in subcutaneous adipose tissue between lean and obese pigs. Mir-9 is more highly expressed in the obese pigs with a fold change of 10 and a p-value < 0.001. Mir-124a is more highly expressed in the obese pigs with a fold change of 114 and a p-value < 0.001. In addition, mir-124a is significantly higher expressed in abdominal adipose tissue in male pigs with a fold change of 119 and a p-value < 0.05. Both miRNAs are also significantly higher expressed in the liver of obese male pigs where mir-124a has a fold change of 12 and mir-9 has a fold change of 1.6, both with p-values < 0.05. Public Library of Science 2015-07-29 /pmc/articles/PMC4519260/ /pubmed/26222688 http://dx.doi.org/10.1371/journal.pone.0131650 Text en © 2015 Mentzel et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mentzel, Caroline M. Junker
Anthon, Christian
Jacobsen, Mette J.
Karlskov-Mortensen, Peter
Bruun, Camilla S.
Jørgensen, Claus B.
Gorodkin, Jan
Cirera, Susanna
Fredholm, Merete
Gender and Obesity Specific MicroRNA Expression in Adipose Tissue from Lean and Obese Pigs
title Gender and Obesity Specific MicroRNA Expression in Adipose Tissue from Lean and Obese Pigs
title_full Gender and Obesity Specific MicroRNA Expression in Adipose Tissue from Lean and Obese Pigs
title_fullStr Gender and Obesity Specific MicroRNA Expression in Adipose Tissue from Lean and Obese Pigs
title_full_unstemmed Gender and Obesity Specific MicroRNA Expression in Adipose Tissue from Lean and Obese Pigs
title_short Gender and Obesity Specific MicroRNA Expression in Adipose Tissue from Lean and Obese Pigs
title_sort gender and obesity specific microrna expression in adipose tissue from lean and obese pigs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519260/
https://www.ncbi.nlm.nih.gov/pubmed/26222688
http://dx.doi.org/10.1371/journal.pone.0131650
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