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MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis

Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters p...

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Autores principales: Tan, Mei-Fang, Gao, Ting, Liu, Wan-Quan, Zhang, Chun-Yan, Yang, Xi, Zhu, Jia-Wen, Teng, Mu-Ye, Li, Lu, Zhou, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519317/
https://www.ncbi.nlm.nih.gov/pubmed/26222651
http://dx.doi.org/10.1371/journal.pone.0130792
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author Tan, Mei-Fang
Gao, Ting
Liu, Wan-Quan
Zhang, Chun-Yan
Yang, Xi
Zhu, Jia-Wen
Teng, Mu-Ye
Li, Lu
Zhou, Rui
author_facet Tan, Mei-Fang
Gao, Ting
Liu, Wan-Quan
Zhang, Chun-Yan
Yang, Xi
Zhu, Jia-Wen
Teng, Mu-Ye
Li, Lu
Zhou, Rui
author_sort Tan, Mei-Fang
collection PubMed
description Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.
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spelling pubmed-45193172015-07-31 MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis Tan, Mei-Fang Gao, Ting Liu, Wan-Quan Zhang, Chun-Yan Yang, Xi Zhu, Jia-Wen Teng, Mu-Ye Li, Lu Zhou, Rui PLoS One Research Article Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen. Public Library of Science 2015-07-29 /pmc/articles/PMC4519317/ /pubmed/26222651 http://dx.doi.org/10.1371/journal.pone.0130792 Text en © 2015 Tan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tan, Mei-Fang
Gao, Ting
Liu, Wan-Quan
Zhang, Chun-Yan
Yang, Xi
Zhu, Jia-Wen
Teng, Mu-Ye
Li, Lu
Zhou, Rui
MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis
title MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis
title_full MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis
title_fullStr MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis
title_full_unstemmed MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis
title_short MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis
title_sort msmk, an atpase, contributes to utilization of multiple carbohydrates and host colonization of streptococcus suis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519317/
https://www.ncbi.nlm.nih.gov/pubmed/26222651
http://dx.doi.org/10.1371/journal.pone.0130792
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