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DNA barcoding of perennial fruit tree species of agronomic interest in the genus Annona (Annonaceae)
The DNA barcode initiative aims to establish a universal protocol using short genetic sequences to discriminate among animal and plant species. Although many markers have been proposed to become the barcode of plants, the Consortium for the Barcode of Life (CBOL) Plant Working Group recommended usin...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519677/ https://www.ncbi.nlm.nih.gov/pubmed/26284104 http://dx.doi.org/10.3389/fpls.2015.00589 |
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author | Larranaga, Nerea Hormaza, José I. |
author_facet | Larranaga, Nerea Hormaza, José I. |
author_sort | Larranaga, Nerea |
collection | PubMed |
description | The DNA barcode initiative aims to establish a universal protocol using short genetic sequences to discriminate among animal and plant species. Although many markers have been proposed to become the barcode of plants, the Consortium for the Barcode of Life (CBOL) Plant Working Group recommended using as a core the combination of two portions of plastid coding region, rbcL and matK. In this paper, specific markers based on matK sequences were developed for 7 closely related Annona species of agronomic interest (Annona cherimola, A. reticulata, A. squamosa, A. muricata, A. macroprophyllata, A. glabra, and A. purpurea) and the discrimination power of both rbcL and matK was tested using also sequences of the genus Annona available in the Barcode of Life Database (BOLD) data systems. The specific sequences developed allowed the discrimination among all those species tested. Moreover, the primers generated were validated in six additional species of the genus (A. liebmanniana, A. longiflora, A. montana, A. senegalensis, A. emarginata and A. neosalicifolia) and in an interspecific hybrid (A. cherimola x A. squamosa). The development of a fast, reliable and economic approach for species identification in these underutilized subtropical fruit crops in a very initial state of domestication is of great importance in order to optimize genetic resource management. |
format | Online Article Text |
id | pubmed-4519677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-45196772015-08-17 DNA barcoding of perennial fruit tree species of agronomic interest in the genus Annona (Annonaceae) Larranaga, Nerea Hormaza, José I. Front Plant Sci Plant Science The DNA barcode initiative aims to establish a universal protocol using short genetic sequences to discriminate among animal and plant species. Although many markers have been proposed to become the barcode of plants, the Consortium for the Barcode of Life (CBOL) Plant Working Group recommended using as a core the combination of two portions of plastid coding region, rbcL and matK. In this paper, specific markers based on matK sequences were developed for 7 closely related Annona species of agronomic interest (Annona cherimola, A. reticulata, A. squamosa, A. muricata, A. macroprophyllata, A. glabra, and A. purpurea) and the discrimination power of both rbcL and matK was tested using also sequences of the genus Annona available in the Barcode of Life Database (BOLD) data systems. The specific sequences developed allowed the discrimination among all those species tested. Moreover, the primers generated were validated in six additional species of the genus (A. liebmanniana, A. longiflora, A. montana, A. senegalensis, A. emarginata and A. neosalicifolia) and in an interspecific hybrid (A. cherimola x A. squamosa). The development of a fast, reliable and economic approach for species identification in these underutilized subtropical fruit crops in a very initial state of domestication is of great importance in order to optimize genetic resource management. Frontiers Media S.A. 2015-07-30 /pmc/articles/PMC4519677/ /pubmed/26284104 http://dx.doi.org/10.3389/fpls.2015.00589 Text en Copyright © 2015 Larranaga and Hormaza. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Larranaga, Nerea Hormaza, José I. DNA barcoding of perennial fruit tree species of agronomic interest in the genus Annona (Annonaceae) |
title | DNA barcoding of perennial fruit tree species of agronomic interest in the genus Annona (Annonaceae) |
title_full | DNA barcoding of perennial fruit tree species of agronomic interest in the genus Annona (Annonaceae) |
title_fullStr | DNA barcoding of perennial fruit tree species of agronomic interest in the genus Annona (Annonaceae) |
title_full_unstemmed | DNA barcoding of perennial fruit tree species of agronomic interest in the genus Annona (Annonaceae) |
title_short | DNA barcoding of perennial fruit tree species of agronomic interest in the genus Annona (Annonaceae) |
title_sort | dna barcoding of perennial fruit tree species of agronomic interest in the genus annona (annonaceae) |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519677/ https://www.ncbi.nlm.nih.gov/pubmed/26284104 http://dx.doi.org/10.3389/fpls.2015.00589 |
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