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A Method for the Immortalization of Newborn Mouse Skin Keratinocytes

Isolation and culture of mouse primary epidermal keratinocytes is a common technique that allows for easy genetic and environmental manipulation. However, due to their limited lifespan in culture, experiments utilizing primary keratinocytes require large numbers of animals, and are time consuming an...

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Detalles Bibliográficos
Autores principales: Hammiller, Brianna O., El-Abaseri, Taghrid Bahig, Dlugosz, Andrzej A., Hansen, Laura A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519689/
https://www.ncbi.nlm.nih.gov/pubmed/26284198
http://dx.doi.org/10.3389/fonc.2015.00177