Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues

We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable...

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Detalles Bibliográficos
Autores principales: Scoville, Steven D., Keller, Karen A., Cheng, Stephanie, Zhang, Michael, Zhang, Xiaoli, Caligiuri, Michael A., Freud, Aharon G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519776/
https://www.ncbi.nlm.nih.gov/pubmed/26223896
http://dx.doi.org/10.1038/srep12490
Descripción
Sumario:We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable, inexpensive, remarkably efficient, and easy to perform, and per sample the overall cost is less than one-tenth the cost associated with a magnetic column-based method.

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