Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues

We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable...

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Autores principales: Scoville, Steven D., Keller, Karen A., Cheng, Stephanie, Zhang, Michael, Zhang, Xiaoli, Caligiuri, Michael A., Freud, Aharon G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519776/
https://www.ncbi.nlm.nih.gov/pubmed/26223896
http://dx.doi.org/10.1038/srep12490
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author Scoville, Steven D.
Keller, Karen A.
Cheng, Stephanie
Zhang, Michael
Zhang, Xiaoli
Caligiuri, Michael A.
Freud, Aharon G.
author_facet Scoville, Steven D.
Keller, Karen A.
Cheng, Stephanie
Zhang, Michael
Zhang, Xiaoli
Caligiuri, Michael A.
Freud, Aharon G.
author_sort Scoville, Steven D.
collection PubMed
description We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable, inexpensive, remarkably efficient, and easy to perform, and per sample the overall cost is less than one-tenth the cost associated with a magnetic column-based method.
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spelling pubmed-45197762015-08-05 Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues Scoville, Steven D. Keller, Karen A. Cheng, Stephanie Zhang, Michael Zhang, Xiaoli Caligiuri, Michael A. Freud, Aharon G. Sci Rep Article We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable, inexpensive, remarkably efficient, and easy to perform, and per sample the overall cost is less than one-tenth the cost associated with a magnetic column-based method. Nature Publishing Group 2015-07-30 /pmc/articles/PMC4519776/ /pubmed/26223896 http://dx.doi.org/10.1038/srep12490 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Scoville, Steven D.
Keller, Karen A.
Cheng, Stephanie
Zhang, Michael
Zhang, Xiaoli
Caligiuri, Michael A.
Freud, Aharon G.
Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues
title Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues
title_full Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues
title_fullStr Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues
title_full_unstemmed Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues
title_short Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues
title_sort rapid column-free enrichment of mononuclear cells from solid tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519776/
https://www.ncbi.nlm.nih.gov/pubmed/26223896
http://dx.doi.org/10.1038/srep12490
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