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BubR1 Acts as a Promoter in Cellular Motility of Human Oral Squamous Cancer Cells through Regulating MMP-2 and MMP-9

BubR1 is a critical component of spindle assembly checkpoint, ensuring proper chromatin segregation during mitosis. Recent studies showed that BubR1 was overexpressed in many cancer cells, including oral squamous cell carcinomas (OSCC). However, the effect of BubR1 on metastasis of OSCC remains uncl...

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Detalles Bibliográficos
Autores principales: Chou, Chou-Kit, Wu, Chang-Yi, Chen, Jeff Yi-Fu, Ng, Ming-Chong, Wang, Hui-Min David, Chen, Jen-Hao, Yuan, Shyng-Shiou F., Tsai, Eing-Mei, Chang, Jan-Gowth, Chiu, Chien-Chih
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519889/
https://www.ncbi.nlm.nih.gov/pubmed/26151845
http://dx.doi.org/10.3390/ijms160715104
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author Chou, Chou-Kit
Wu, Chang-Yi
Chen, Jeff Yi-Fu
Ng, Ming-Chong
Wang, Hui-Min David
Chen, Jen-Hao
Yuan, Shyng-Shiou F.
Tsai, Eing-Mei
Chang, Jan-Gowth
Chiu, Chien-Chih
author_facet Chou, Chou-Kit
Wu, Chang-Yi
Chen, Jeff Yi-Fu
Ng, Ming-Chong
Wang, Hui-Min David
Chen, Jen-Hao
Yuan, Shyng-Shiou F.
Tsai, Eing-Mei
Chang, Jan-Gowth
Chiu, Chien-Chih
author_sort Chou, Chou-Kit
collection PubMed
description BubR1 is a critical component of spindle assembly checkpoint, ensuring proper chromatin segregation during mitosis. Recent studies showed that BubR1 was overexpressed in many cancer cells, including oral squamous cell carcinomas (OSCC). However, the effect of BubR1 on metastasis of OSCC remains unclear. This study aimed to unravel the role of BubR1 in the progression of OSCC and confirm the expression of BubR1 in a panel of malignant OSCC cell lines with different invasive abilities. The results of quantitative real-time PCR showed that the mRNA level of BubR1 was markedly increased in four OSCC cell lines, Ca9-22, HSC3, SCC9 and Cal-27 cells, compared to two normal cells, normal human oral keratinocytes (HOK) and human gingival fibroblasts (HGF). Moreover, the expression of BubR1 in these four OSCC cell lines was positively correlated with their motility. Immunofluorescence revealed that BubR1 was mostly localized in the cytosol of human gingival carcinoma Ca9-22 cells. BubR1 knockdown significantly decreased cellular invasion but slightly affect cellular proliferation on both Ca9-22 and Cal-27 cells. Consistently, the activities of metastasis-associated metalloproteinases MMP-2 and MMP-9 were attenuated in BubR1 knockdown Ca9-22 cells, suggesting the role of BubR1 in promotion of OSCC migration. Our present study defines an alternative pathway in promoting metastasis of OSCC cells, and the expression of BubR1 could be a prognostic index in OSCC patients.
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spelling pubmed-45198892015-08-03 BubR1 Acts as a Promoter in Cellular Motility of Human Oral Squamous Cancer Cells through Regulating MMP-2 and MMP-9 Chou, Chou-Kit Wu, Chang-Yi Chen, Jeff Yi-Fu Ng, Ming-Chong Wang, Hui-Min David Chen, Jen-Hao Yuan, Shyng-Shiou F. Tsai, Eing-Mei Chang, Jan-Gowth Chiu, Chien-Chih Int J Mol Sci Article BubR1 is a critical component of spindle assembly checkpoint, ensuring proper chromatin segregation during mitosis. Recent studies showed that BubR1 was overexpressed in many cancer cells, including oral squamous cell carcinomas (OSCC). However, the effect of BubR1 on metastasis of OSCC remains unclear. This study aimed to unravel the role of BubR1 in the progression of OSCC and confirm the expression of BubR1 in a panel of malignant OSCC cell lines with different invasive abilities. The results of quantitative real-time PCR showed that the mRNA level of BubR1 was markedly increased in four OSCC cell lines, Ca9-22, HSC3, SCC9 and Cal-27 cells, compared to two normal cells, normal human oral keratinocytes (HOK) and human gingival fibroblasts (HGF). Moreover, the expression of BubR1 in these four OSCC cell lines was positively correlated with their motility. Immunofluorescence revealed that BubR1 was mostly localized in the cytosol of human gingival carcinoma Ca9-22 cells. BubR1 knockdown significantly decreased cellular invasion but slightly affect cellular proliferation on both Ca9-22 and Cal-27 cells. Consistently, the activities of metastasis-associated metalloproteinases MMP-2 and MMP-9 were attenuated in BubR1 knockdown Ca9-22 cells, suggesting the role of BubR1 in promotion of OSCC migration. Our present study defines an alternative pathway in promoting metastasis of OSCC cells, and the expression of BubR1 could be a prognostic index in OSCC patients. MDPI 2015-07-03 /pmc/articles/PMC4519889/ /pubmed/26151845 http://dx.doi.org/10.3390/ijms160715104 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chou, Chou-Kit
Wu, Chang-Yi
Chen, Jeff Yi-Fu
Ng, Ming-Chong
Wang, Hui-Min David
Chen, Jen-Hao
Yuan, Shyng-Shiou F.
Tsai, Eing-Mei
Chang, Jan-Gowth
Chiu, Chien-Chih
BubR1 Acts as a Promoter in Cellular Motility of Human Oral Squamous Cancer Cells through Regulating MMP-2 and MMP-9
title BubR1 Acts as a Promoter in Cellular Motility of Human Oral Squamous Cancer Cells through Regulating MMP-2 and MMP-9
title_full BubR1 Acts as a Promoter in Cellular Motility of Human Oral Squamous Cancer Cells through Regulating MMP-2 and MMP-9
title_fullStr BubR1 Acts as a Promoter in Cellular Motility of Human Oral Squamous Cancer Cells through Regulating MMP-2 and MMP-9
title_full_unstemmed BubR1 Acts as a Promoter in Cellular Motility of Human Oral Squamous Cancer Cells through Regulating MMP-2 and MMP-9
title_short BubR1 Acts as a Promoter in Cellular Motility of Human Oral Squamous Cancer Cells through Regulating MMP-2 and MMP-9
title_sort bubr1 acts as a promoter in cellular motility of human oral squamous cancer cells through regulating mmp-2 and mmp-9
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519889/
https://www.ncbi.nlm.nih.gov/pubmed/26151845
http://dx.doi.org/10.3390/ijms160715104
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