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Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities

Filamentous fungi produce a great variety of enzymes, and research on their biotechnological potential has recently intensified. The objective of this work was to identify, at the species level, using DNA barcoding, 46 fungal isolates obtained from maize grains with rot symptoms. We also analyzed th...

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Autores principales: Lumi Abe, Camila Agnes, Bertechini Faria, Carla, Fernandes de Castro, Fausto, de Souza, Sandra Regina, dos Santos, Fabiane Cristina, Novais da Silva, Cleiltan, Tessmann, Dauri José, Barbosa-Tessmann, Ione Parra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519902/
https://www.ncbi.nlm.nih.gov/pubmed/26198227
http://dx.doi.org/10.3390/ijms160715328
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author Lumi Abe, Camila Agnes
Bertechini Faria, Carla
Fernandes de Castro, Fausto
de Souza, Sandra Regina
dos Santos, Fabiane Cristina
Novais da Silva, Cleiltan
Tessmann, Dauri José
Barbosa-Tessmann, Ione Parra
author_facet Lumi Abe, Camila Agnes
Bertechini Faria, Carla
Fernandes de Castro, Fausto
de Souza, Sandra Regina
dos Santos, Fabiane Cristina
Novais da Silva, Cleiltan
Tessmann, Dauri José
Barbosa-Tessmann, Ione Parra
author_sort Lumi Abe, Camila Agnes
collection PubMed
description Filamentous fungi produce a great variety of enzymes, and research on their biotechnological potential has recently intensified. The objective of this work was to identify, at the species level, using DNA barcoding, 46 fungal isolates obtained from maize grains with rot symptoms. We also analyzed the production of extracellular amylases, cellulases, proteases and lipases of 33 of those fungal isolates. The enzymatic activities were evaluated by the formation of a clear halo or a white precipitate around the colonies in defined substrate media. The found fungi belong to the genera Talaromyces, Stenocarpella, Penicillium, Phlebiopsis, Cladosporium, Hyphopichia, Epicoccum, Trichoderma, Aspergillus, Irpex, Fusarium, Microdochium, Mucor and Sarocladium. In the genus Fusarium, the species Fusarium verticillioides was predominant and this genus presented the highest diversity, followed by the genera Aspergillus. The best genera for lipase production were Cladosporium and Penicillium; while Cladosporium, Aspergillus and Penicillium were best for cellulase activity; Hyphopichia, Aspergillus and Irpex for amylase activity; and Cladosporium and Sarocladium for proteases activity. In conclusion, a collection of fungi from maize seeds presenting rotten symptoms were obtained, among which exist important producers of hydrolases.
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spelling pubmed-45199022015-08-03 Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities Lumi Abe, Camila Agnes Bertechini Faria, Carla Fernandes de Castro, Fausto de Souza, Sandra Regina dos Santos, Fabiane Cristina Novais da Silva, Cleiltan Tessmann, Dauri José Barbosa-Tessmann, Ione Parra Int J Mol Sci Article Filamentous fungi produce a great variety of enzymes, and research on their biotechnological potential has recently intensified. The objective of this work was to identify, at the species level, using DNA barcoding, 46 fungal isolates obtained from maize grains with rot symptoms. We also analyzed the production of extracellular amylases, cellulases, proteases and lipases of 33 of those fungal isolates. The enzymatic activities were evaluated by the formation of a clear halo or a white precipitate around the colonies in defined substrate media. The found fungi belong to the genera Talaromyces, Stenocarpella, Penicillium, Phlebiopsis, Cladosporium, Hyphopichia, Epicoccum, Trichoderma, Aspergillus, Irpex, Fusarium, Microdochium, Mucor and Sarocladium. In the genus Fusarium, the species Fusarium verticillioides was predominant and this genus presented the highest diversity, followed by the genera Aspergillus. The best genera for lipase production were Cladosporium and Penicillium; while Cladosporium, Aspergillus and Penicillium were best for cellulase activity; Hyphopichia, Aspergillus and Irpex for amylase activity; and Cladosporium and Sarocladium for proteases activity. In conclusion, a collection of fungi from maize seeds presenting rotten symptoms were obtained, among which exist important producers of hydrolases. MDPI 2015-07-07 /pmc/articles/PMC4519902/ /pubmed/26198227 http://dx.doi.org/10.3390/ijms160715328 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lumi Abe, Camila Agnes
Bertechini Faria, Carla
Fernandes de Castro, Fausto
de Souza, Sandra Regina
dos Santos, Fabiane Cristina
Novais da Silva, Cleiltan
Tessmann, Dauri José
Barbosa-Tessmann, Ione Parra
Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities
title Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities
title_full Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities
title_fullStr Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities
title_full_unstemmed Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities
title_short Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities
title_sort fungi isolated from maize (zea mays l.) grains and production of associated enzyme activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519902/
https://www.ncbi.nlm.nih.gov/pubmed/26198227
http://dx.doi.org/10.3390/ijms160715328
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