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Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities
Filamentous fungi produce a great variety of enzymes, and research on their biotechnological potential has recently intensified. The objective of this work was to identify, at the species level, using DNA barcoding, 46 fungal isolates obtained from maize grains with rot symptoms. We also analyzed th...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519902/ https://www.ncbi.nlm.nih.gov/pubmed/26198227 http://dx.doi.org/10.3390/ijms160715328 |
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author | Lumi Abe, Camila Agnes Bertechini Faria, Carla Fernandes de Castro, Fausto de Souza, Sandra Regina dos Santos, Fabiane Cristina Novais da Silva, Cleiltan Tessmann, Dauri José Barbosa-Tessmann, Ione Parra |
author_facet | Lumi Abe, Camila Agnes Bertechini Faria, Carla Fernandes de Castro, Fausto de Souza, Sandra Regina dos Santos, Fabiane Cristina Novais da Silva, Cleiltan Tessmann, Dauri José Barbosa-Tessmann, Ione Parra |
author_sort | Lumi Abe, Camila Agnes |
collection | PubMed |
description | Filamentous fungi produce a great variety of enzymes, and research on their biotechnological potential has recently intensified. The objective of this work was to identify, at the species level, using DNA barcoding, 46 fungal isolates obtained from maize grains with rot symptoms. We also analyzed the production of extracellular amylases, cellulases, proteases and lipases of 33 of those fungal isolates. The enzymatic activities were evaluated by the formation of a clear halo or a white precipitate around the colonies in defined substrate media. The found fungi belong to the genera Talaromyces, Stenocarpella, Penicillium, Phlebiopsis, Cladosporium, Hyphopichia, Epicoccum, Trichoderma, Aspergillus, Irpex, Fusarium, Microdochium, Mucor and Sarocladium. In the genus Fusarium, the species Fusarium verticillioides was predominant and this genus presented the highest diversity, followed by the genera Aspergillus. The best genera for lipase production were Cladosporium and Penicillium; while Cladosporium, Aspergillus and Penicillium were best for cellulase activity; Hyphopichia, Aspergillus and Irpex for amylase activity; and Cladosporium and Sarocladium for proteases activity. In conclusion, a collection of fungi from maize seeds presenting rotten symptoms were obtained, among which exist important producers of hydrolases. |
format | Online Article Text |
id | pubmed-4519902 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-45199022015-08-03 Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities Lumi Abe, Camila Agnes Bertechini Faria, Carla Fernandes de Castro, Fausto de Souza, Sandra Regina dos Santos, Fabiane Cristina Novais da Silva, Cleiltan Tessmann, Dauri José Barbosa-Tessmann, Ione Parra Int J Mol Sci Article Filamentous fungi produce a great variety of enzymes, and research on their biotechnological potential has recently intensified. The objective of this work was to identify, at the species level, using DNA barcoding, 46 fungal isolates obtained from maize grains with rot symptoms. We also analyzed the production of extracellular amylases, cellulases, proteases and lipases of 33 of those fungal isolates. The enzymatic activities were evaluated by the formation of a clear halo or a white precipitate around the colonies in defined substrate media. The found fungi belong to the genera Talaromyces, Stenocarpella, Penicillium, Phlebiopsis, Cladosporium, Hyphopichia, Epicoccum, Trichoderma, Aspergillus, Irpex, Fusarium, Microdochium, Mucor and Sarocladium. In the genus Fusarium, the species Fusarium verticillioides was predominant and this genus presented the highest diversity, followed by the genera Aspergillus. The best genera for lipase production were Cladosporium and Penicillium; while Cladosporium, Aspergillus and Penicillium were best for cellulase activity; Hyphopichia, Aspergillus and Irpex for amylase activity; and Cladosporium and Sarocladium for proteases activity. In conclusion, a collection of fungi from maize seeds presenting rotten symptoms were obtained, among which exist important producers of hydrolases. MDPI 2015-07-07 /pmc/articles/PMC4519902/ /pubmed/26198227 http://dx.doi.org/10.3390/ijms160715328 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lumi Abe, Camila Agnes Bertechini Faria, Carla Fernandes de Castro, Fausto de Souza, Sandra Regina dos Santos, Fabiane Cristina Novais da Silva, Cleiltan Tessmann, Dauri José Barbosa-Tessmann, Ione Parra Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities |
title | Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities |
title_full | Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities |
title_fullStr | Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities |
title_full_unstemmed | Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities |
title_short | Fungi Isolated from Maize (Zea mays L.) Grains and Production of Associated Enzyme Activities |
title_sort | fungi isolated from maize (zea mays l.) grains and production of associated enzyme activities |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519902/ https://www.ncbi.nlm.nih.gov/pubmed/26198227 http://dx.doi.org/10.3390/ijms160715328 |
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