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Purification and Structural Identification of Polysaccharides from Bamboo Shoots (Dendrocalamus latiflorus)

Three kinds of polysaccharides, namely, BSP1A, BSP2A, and BSP3B, were isolated from raw bamboo shoot (Dendrocalamus latiflorus) after purification and classification by DEAE cellulose-52 (ion-exchange chromatography) and Sephadex G-50. The molecular weights of BSP1A, BSP2A, and BSP3B were 10.2, 17.0...

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Autores principales: Wu, Jinsong, Zheng, Jiong, Xia, Xuejuan, Kan, Jianquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519913/
https://www.ncbi.nlm.nih.gov/pubmed/26184163
http://dx.doi.org/10.3390/ijms160715560
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author Wu, Jinsong
Zheng, Jiong
Xia, Xuejuan
Kan, Jianquan
author_facet Wu, Jinsong
Zheng, Jiong
Xia, Xuejuan
Kan, Jianquan
author_sort Wu, Jinsong
collection PubMed
description Three kinds of polysaccharides, namely, BSP1A, BSP2A, and BSP3B, were isolated from raw bamboo shoot (Dendrocalamus latiflorus) after purification and classification by DEAE cellulose-52 (ion-exchange chromatography) and Sephadex G-50. The molecular weights of BSP1A, BSP2A, and BSP3B were 10.2, 17.0 and 20.0 kDa, respectively, which were measured through GPC (gel performance chromtatography) methods. BSP1A contained arabinose, glucose, and galactose in a molar ratio of 1.0:40.6:8.7. BSP2A and BSP3B contained arabinose, xylose, glucose, and galactose in molar ratios of 6.6:1.0:5.2:10.4 and 8.5:1.0:5.1:11.1, respectively. The existence of the O-glycopeptide bond in BSP1A, BSP2A, and BSP3B was demonstrated by β-elimination reaction. FTIR spectra of the three polysaccharides showed that both BSP2A and BSP3B contained β-d-pyranose sugar rings. However, BSP1A exhibited both β-d-pyranose and α-d-pyranose sugar rings. Congo red test indicated that BSP1A and BSP2A displayed triple helix structures, but BSP3B did not. NMR spectroscopy revealed that BSP1A may exhibit a β-1,6-Glucan pyran type as the main link, and few 1,6-glycosidic galactose pyranose and arabinose bonds were connected; BSP2A mainly demonstrated →5)β-Ara(1→and→3)β-Gal(1→connection. Furthermore, BSP3B mainly presented →3)β-Glu(1→and→3)β-Gal(1→connection and may also contain few other glycosidic bonds.
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spelling pubmed-45199132015-08-03 Purification and Structural Identification of Polysaccharides from Bamboo Shoots (Dendrocalamus latiflorus) Wu, Jinsong Zheng, Jiong Xia, Xuejuan Kan, Jianquan Int J Mol Sci Article Three kinds of polysaccharides, namely, BSP1A, BSP2A, and BSP3B, were isolated from raw bamboo shoot (Dendrocalamus latiflorus) after purification and classification by DEAE cellulose-52 (ion-exchange chromatography) and Sephadex G-50. The molecular weights of BSP1A, BSP2A, and BSP3B were 10.2, 17.0 and 20.0 kDa, respectively, which were measured through GPC (gel performance chromtatography) methods. BSP1A contained arabinose, glucose, and galactose in a molar ratio of 1.0:40.6:8.7. BSP2A and BSP3B contained arabinose, xylose, glucose, and galactose in molar ratios of 6.6:1.0:5.2:10.4 and 8.5:1.0:5.1:11.1, respectively. The existence of the O-glycopeptide bond in BSP1A, BSP2A, and BSP3B was demonstrated by β-elimination reaction. FTIR spectra of the three polysaccharides showed that both BSP2A and BSP3B contained β-d-pyranose sugar rings. However, BSP1A exhibited both β-d-pyranose and α-d-pyranose sugar rings. Congo red test indicated that BSP1A and BSP2A displayed triple helix structures, but BSP3B did not. NMR spectroscopy revealed that BSP1A may exhibit a β-1,6-Glucan pyran type as the main link, and few 1,6-glycosidic galactose pyranose and arabinose bonds were connected; BSP2A mainly demonstrated →5)β-Ara(1→and→3)β-Gal(1→connection. Furthermore, BSP3B mainly presented →3)β-Glu(1→and→3)β-Gal(1→connection and may also contain few other glycosidic bonds. MDPI 2015-07-09 /pmc/articles/PMC4519913/ /pubmed/26184163 http://dx.doi.org/10.3390/ijms160715560 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Jinsong
Zheng, Jiong
Xia, Xuejuan
Kan, Jianquan
Purification and Structural Identification of Polysaccharides from Bamboo Shoots (Dendrocalamus latiflorus)
title Purification and Structural Identification of Polysaccharides from Bamboo Shoots (Dendrocalamus latiflorus)
title_full Purification and Structural Identification of Polysaccharides from Bamboo Shoots (Dendrocalamus latiflorus)
title_fullStr Purification and Structural Identification of Polysaccharides from Bamboo Shoots (Dendrocalamus latiflorus)
title_full_unstemmed Purification and Structural Identification of Polysaccharides from Bamboo Shoots (Dendrocalamus latiflorus)
title_short Purification and Structural Identification of Polysaccharides from Bamboo Shoots (Dendrocalamus latiflorus)
title_sort purification and structural identification of polysaccharides from bamboo shoots (dendrocalamus latiflorus)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519913/
https://www.ncbi.nlm.nih.gov/pubmed/26184163
http://dx.doi.org/10.3390/ijms160715560
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