Cargando…

Transcriptome Profile Analysis of Ovarian Tissues from Diploid and Tetraploid Loaches Misgurnus anguillicaudatus

RNA sequencing and short-read assembly was utilized to produce a transcriptome of ovarian tissues from three-year-old diploid and tetraploid loaches (Misgurnus anguillicaudatus). A total of 28,369 unigenes were obtained, comprising 10,546 unigenes with length longer than 1000 bp. More than 73% of th...

Descripción completa

Detalles Bibliográficos
Autores principales: Luo, Weiwei, Liu, Chuanshu, Cao, Xiaojuan, Huang, Songqian, Wang, Weimin, Wang, Yeke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519936/
https://www.ncbi.nlm.nih.gov/pubmed/26184186
http://dx.doi.org/10.3390/ijms160716017
_version_ 1782383582696701952
author Luo, Weiwei
Liu, Chuanshu
Cao, Xiaojuan
Huang, Songqian
Wang, Weimin
Wang, Yeke
author_facet Luo, Weiwei
Liu, Chuanshu
Cao, Xiaojuan
Huang, Songqian
Wang, Weimin
Wang, Yeke
author_sort Luo, Weiwei
collection PubMed
description RNA sequencing and short-read assembly was utilized to produce a transcriptome of ovarian tissues from three-year-old diploid and tetraploid loaches (Misgurnus anguillicaudatus). A total of 28,369 unigenes were obtained, comprising 10,546 unigenes with length longer than 1000 bp. More than 73% of the unigenes were annotated through sequence comparison with databases. The RNA-seq data revealed that 2253 genes were differentially expressed between diploid and tetraploid loaches, including 1263 up-regulated and 990 down-regulated genes in tetraploid loach. Some differentially expressed genes, such as vitellogenin (Vtg), gonadotropin releasing hormone receptor type A (GnRHRA), steroidogenic acute regulatory protein (StAR), mitogen-activated protein kinase 14a (MAPK14a), ATP synthase subunit alpha (atp5a), and synaptonemal complex protein 1 (Scp1), were involved in regulation of cell proliferation, division, gene transcription, ovarian development and energy metabolism, suggesting that these genes were related to egg diameter of the loach. Results of transcriptome profiling here were validated using real time quantitative PCR in ten selected genes. The present study provided insights into the transcriptome profile of ovarian tissues from diploid and tetraploid loaches Misgurnus anguillicaudatus, which was made available to the research community for functional genomics, comparative genomics, polyploidy evolution and molecular breeding of this loach and other related species.
format Online
Article
Text
id pubmed-4519936
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-45199362015-08-03 Transcriptome Profile Analysis of Ovarian Tissues from Diploid and Tetraploid Loaches Misgurnus anguillicaudatus Luo, Weiwei Liu, Chuanshu Cao, Xiaojuan Huang, Songqian Wang, Weimin Wang, Yeke Int J Mol Sci Article RNA sequencing and short-read assembly was utilized to produce a transcriptome of ovarian tissues from three-year-old diploid and tetraploid loaches (Misgurnus anguillicaudatus). A total of 28,369 unigenes were obtained, comprising 10,546 unigenes with length longer than 1000 bp. More than 73% of the unigenes were annotated through sequence comparison with databases. The RNA-seq data revealed that 2253 genes were differentially expressed between diploid and tetraploid loaches, including 1263 up-regulated and 990 down-regulated genes in tetraploid loach. Some differentially expressed genes, such as vitellogenin (Vtg), gonadotropin releasing hormone receptor type A (GnRHRA), steroidogenic acute regulatory protein (StAR), mitogen-activated protein kinase 14a (MAPK14a), ATP synthase subunit alpha (atp5a), and synaptonemal complex protein 1 (Scp1), were involved in regulation of cell proliferation, division, gene transcription, ovarian development and energy metabolism, suggesting that these genes were related to egg diameter of the loach. Results of transcriptome profiling here were validated using real time quantitative PCR in ten selected genes. The present study provided insights into the transcriptome profile of ovarian tissues from diploid and tetraploid loaches Misgurnus anguillicaudatus, which was made available to the research community for functional genomics, comparative genomics, polyploidy evolution and molecular breeding of this loach and other related species. MDPI 2015-07-14 /pmc/articles/PMC4519936/ /pubmed/26184186 http://dx.doi.org/10.3390/ijms160716017 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luo, Weiwei
Liu, Chuanshu
Cao, Xiaojuan
Huang, Songqian
Wang, Weimin
Wang, Yeke
Transcriptome Profile Analysis of Ovarian Tissues from Diploid and Tetraploid Loaches Misgurnus anguillicaudatus
title Transcriptome Profile Analysis of Ovarian Tissues from Diploid and Tetraploid Loaches Misgurnus anguillicaudatus
title_full Transcriptome Profile Analysis of Ovarian Tissues from Diploid and Tetraploid Loaches Misgurnus anguillicaudatus
title_fullStr Transcriptome Profile Analysis of Ovarian Tissues from Diploid and Tetraploid Loaches Misgurnus anguillicaudatus
title_full_unstemmed Transcriptome Profile Analysis of Ovarian Tissues from Diploid and Tetraploid Loaches Misgurnus anguillicaudatus
title_short Transcriptome Profile Analysis of Ovarian Tissues from Diploid and Tetraploid Loaches Misgurnus anguillicaudatus
title_sort transcriptome profile analysis of ovarian tissues from diploid and tetraploid loaches misgurnus anguillicaudatus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519936/
https://www.ncbi.nlm.nih.gov/pubmed/26184186
http://dx.doi.org/10.3390/ijms160716017
work_keys_str_mv AT luoweiwei transcriptomeprofileanalysisofovariantissuesfromdiploidandtetraploidloachesmisgurnusanguillicaudatus
AT liuchuanshu transcriptomeprofileanalysisofovariantissuesfromdiploidandtetraploidloachesmisgurnusanguillicaudatus
AT caoxiaojuan transcriptomeprofileanalysisofovariantissuesfromdiploidandtetraploidloachesmisgurnusanguillicaudatus
AT huangsongqian transcriptomeprofileanalysisofovariantissuesfromdiploidandtetraploidloachesmisgurnusanguillicaudatus
AT wangweimin transcriptomeprofileanalysisofovariantissuesfromdiploidandtetraploidloachesmisgurnusanguillicaudatus
AT wangyeke transcriptomeprofileanalysisofovariantissuesfromdiploidandtetraploidloachesmisgurnusanguillicaudatus