Cargando…
Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production
The objective of this study was to investigate the effects of indole-3-acetic acid (IAA) and kinetin (KIN) on Mucor indicus growth, cell wall composition, and ethanol production. A semi-synthetic medium, supplemented with 0–5 mg/L hormones, was used for the cultivations (at 32 °C for 48 h). By addit...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519973/ https://www.ncbi.nlm.nih.gov/pubmed/26204839 http://dx.doi.org/10.3390/ijms160716683 |
_version_ | 1782383591061192704 |
---|---|
author | Safaei, Zahra Karimi, Keikhosro Golkar, Poorandokht Zamani, Akram |
author_facet | Safaei, Zahra Karimi, Keikhosro Golkar, Poorandokht Zamani, Akram |
author_sort | Safaei, Zahra |
collection | PubMed |
description | The objective of this study was to investigate the effects of indole-3-acetic acid (IAA) and kinetin (KIN) on Mucor indicus growth, cell wall composition, and ethanol production. A semi-synthetic medium, supplemented with 0–5 mg/L hormones, was used for the cultivations (at 32 °C for 48 h). By addition of 1 mg/L of each hormone, the biomass and ethanol yields were increased and decreased, respectively. At higher levels, however, an inverse trend was observed. The glucosamine fraction of the cell wall, as a representative for chitosan, followed similar but sharper changes, compared to the biomass. The highest level was 221% higher than that obtained without hormones. The sum of glucosamine and N-acetyl glucosamine (chitin and chitosan) was noticeably enhanced in the presence of the hormones. Increase of chitosan was accompanied by a decrease in the phosphate content, with the lowest phosphate (0.01 g/g cell wall) being obtained when the chitosan was at the maximum (0.45 g/g cell wall). In conclusion, IAA and KIN significantly enhanced the M. indicus growth and chitosan production, while at the same time decreasing the ethanol yield to some extent. This study shows that plant growth hormones have a high potential for the improvement of fungal chitosan production by M. indicus. |
format | Online Article Text |
id | pubmed-4519973 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-45199732015-08-03 Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production Safaei, Zahra Karimi, Keikhosro Golkar, Poorandokht Zamani, Akram Int J Mol Sci Article The objective of this study was to investigate the effects of indole-3-acetic acid (IAA) and kinetin (KIN) on Mucor indicus growth, cell wall composition, and ethanol production. A semi-synthetic medium, supplemented with 0–5 mg/L hormones, was used for the cultivations (at 32 °C for 48 h). By addition of 1 mg/L of each hormone, the biomass and ethanol yields were increased and decreased, respectively. At higher levels, however, an inverse trend was observed. The glucosamine fraction of the cell wall, as a representative for chitosan, followed similar but sharper changes, compared to the biomass. The highest level was 221% higher than that obtained without hormones. The sum of glucosamine and N-acetyl glucosamine (chitin and chitosan) was noticeably enhanced in the presence of the hormones. Increase of chitosan was accompanied by a decrease in the phosphate content, with the lowest phosphate (0.01 g/g cell wall) being obtained when the chitosan was at the maximum (0.45 g/g cell wall). In conclusion, IAA and KIN significantly enhanced the M. indicus growth and chitosan production, while at the same time decreasing the ethanol yield to some extent. This study shows that plant growth hormones have a high potential for the improvement of fungal chitosan production by M. indicus. MDPI 2015-07-22 /pmc/articles/PMC4519973/ /pubmed/26204839 http://dx.doi.org/10.3390/ijms160716683 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Safaei, Zahra Karimi, Keikhosro Golkar, Poorandokht Zamani, Akram Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production |
title | Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production |
title_full | Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production |
title_fullStr | Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production |
title_full_unstemmed | Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production |
title_short | Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production |
title_sort | effects of plant growth hormones on mucor indicus growth and chitosan and ethanol production |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519973/ https://www.ncbi.nlm.nih.gov/pubmed/26204839 http://dx.doi.org/10.3390/ijms160716683 |
work_keys_str_mv | AT safaeizahra effectsofplantgrowthhormonesonmucorindicusgrowthandchitosanandethanolproduction AT karimikeikhosro effectsofplantgrowthhormonesonmucorindicusgrowthandchitosanandethanolproduction AT golkarpoorandokht effectsofplantgrowthhormonesonmucorindicusgrowthandchitosanandethanolproduction AT zamaniakram effectsofplantgrowthhormonesonmucorindicusgrowthandchitosanandethanolproduction |