Cargando…

Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production

The objective of this study was to investigate the effects of indole-3-acetic acid (IAA) and kinetin (KIN) on Mucor indicus growth, cell wall composition, and ethanol production. A semi-synthetic medium, supplemented with 0–5 mg/L hormones, was used for the cultivations (at 32 °C for 48 h). By addit...

Descripción completa

Detalles Bibliográficos
Autores principales: Safaei, Zahra, Karimi, Keikhosro, Golkar, Poorandokht, Zamani, Akram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519973/
https://www.ncbi.nlm.nih.gov/pubmed/26204839
http://dx.doi.org/10.3390/ijms160716683
_version_ 1782383591061192704
author Safaei, Zahra
Karimi, Keikhosro
Golkar, Poorandokht
Zamani, Akram
author_facet Safaei, Zahra
Karimi, Keikhosro
Golkar, Poorandokht
Zamani, Akram
author_sort Safaei, Zahra
collection PubMed
description The objective of this study was to investigate the effects of indole-3-acetic acid (IAA) and kinetin (KIN) on Mucor indicus growth, cell wall composition, and ethanol production. A semi-synthetic medium, supplemented with 0–5 mg/L hormones, was used for the cultivations (at 32 °C for 48 h). By addition of 1 mg/L of each hormone, the biomass and ethanol yields were increased and decreased, respectively. At higher levels, however, an inverse trend was observed. The glucosamine fraction of the cell wall, as a representative for chitosan, followed similar but sharper changes, compared to the biomass. The highest level was 221% higher than that obtained without hormones. The sum of glucosamine and N-acetyl glucosamine (chitin and chitosan) was noticeably enhanced in the presence of the hormones. Increase of chitosan was accompanied by a decrease in the phosphate content, with the lowest phosphate (0.01 g/g cell wall) being obtained when the chitosan was at the maximum (0.45 g/g cell wall). In conclusion, IAA and KIN significantly enhanced the M. indicus growth and chitosan production, while at the same time decreasing the ethanol yield to some extent. This study shows that plant growth hormones have a high potential for the improvement of fungal chitosan production by M. indicus.
format Online
Article
Text
id pubmed-4519973
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-45199732015-08-03 Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production Safaei, Zahra Karimi, Keikhosro Golkar, Poorandokht Zamani, Akram Int J Mol Sci Article The objective of this study was to investigate the effects of indole-3-acetic acid (IAA) and kinetin (KIN) on Mucor indicus growth, cell wall composition, and ethanol production. A semi-synthetic medium, supplemented with 0–5 mg/L hormones, was used for the cultivations (at 32 °C for 48 h). By addition of 1 mg/L of each hormone, the biomass and ethanol yields were increased and decreased, respectively. At higher levels, however, an inverse trend was observed. The glucosamine fraction of the cell wall, as a representative for chitosan, followed similar but sharper changes, compared to the biomass. The highest level was 221% higher than that obtained without hormones. The sum of glucosamine and N-acetyl glucosamine (chitin and chitosan) was noticeably enhanced in the presence of the hormones. Increase of chitosan was accompanied by a decrease in the phosphate content, with the lowest phosphate (0.01 g/g cell wall) being obtained when the chitosan was at the maximum (0.45 g/g cell wall). In conclusion, IAA and KIN significantly enhanced the M. indicus growth and chitosan production, while at the same time decreasing the ethanol yield to some extent. This study shows that plant growth hormones have a high potential for the improvement of fungal chitosan production by M. indicus. MDPI 2015-07-22 /pmc/articles/PMC4519973/ /pubmed/26204839 http://dx.doi.org/10.3390/ijms160716683 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Safaei, Zahra
Karimi, Keikhosro
Golkar, Poorandokht
Zamani, Akram
Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production
title Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production
title_full Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production
title_fullStr Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production
title_full_unstemmed Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production
title_short Effects of Plant Growth Hormones on Mucor indicus Growth and Chitosan and Ethanol Production
title_sort effects of plant growth hormones on mucor indicus growth and chitosan and ethanol production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519973/
https://www.ncbi.nlm.nih.gov/pubmed/26204839
http://dx.doi.org/10.3390/ijms160716683
work_keys_str_mv AT safaeizahra effectsofplantgrowthhormonesonmucorindicusgrowthandchitosanandethanolproduction
AT karimikeikhosro effectsofplantgrowthhormonesonmucorindicusgrowthandchitosanandethanolproduction
AT golkarpoorandokht effectsofplantgrowthhormonesonmucorindicusgrowthandchitosanandethanolproduction
AT zamaniakram effectsofplantgrowthhormonesonmucorindicusgrowthandchitosanandethanolproduction