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Biocompatibility of Polypyrrole with Human Primary Osteoblasts and the Effect of Dopants

Polypyrrole (PPy) is a conducting polymer that enables controlled drug release upon electrical stimulation. We characterized the biocompatibility of PPy with human primary osteoblasts, and the effect of dopants. We investigated the biocompatibility of PPy comprising various dopants, i.e. p-toluene s...

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Autores principales: Fahlgren, Anna, Bratengeier, Cornelia, Gelmi, Amy, Semeins, Cornelis M., Klein-Nulend, Jenneke, Jager, Edwin W. H., Bakker, Astrid D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4520445/
https://www.ncbi.nlm.nih.gov/pubmed/26225862
http://dx.doi.org/10.1371/journal.pone.0134023
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author Fahlgren, Anna
Bratengeier, Cornelia
Gelmi, Amy
Semeins, Cornelis M.
Klein-Nulend, Jenneke
Jager, Edwin W. H.
Bakker, Astrid D.
author_facet Fahlgren, Anna
Bratengeier, Cornelia
Gelmi, Amy
Semeins, Cornelis M.
Klein-Nulend, Jenneke
Jager, Edwin W. H.
Bakker, Astrid D.
author_sort Fahlgren, Anna
collection PubMed
description Polypyrrole (PPy) is a conducting polymer that enables controlled drug release upon electrical stimulation. We characterized the biocompatibility of PPy with human primary osteoblasts, and the effect of dopants. We investigated the biocompatibility of PPy comprising various dopants, i.e. p-toluene sulfonate (PPy-pTS), chondroitin sulfate (PPy-CS), or dodecylbenzenesulfonate (PPy-DBS), with human primary osteoblasts. PPy-DBS showed the roughest appearance of all surfaces tested, and its wettability was similar to the gold-coated control. The average number of attached cells was 45% higher on PPy-DBS than on PPy-CS or PPy-pTS, although gene expression of the proliferation marker Ki-67 was similar in osteoblasts on all surfaces tested. Osteoblasts seeded on PPy-DBS or gold showed similar vinculin attachment points, vinculin area per cell area, actin filament structure, and Feret’s diameter, while cells seeded on PPY-CS or PPY-pTS showed disturbed focal adhesions and were enlarged with disorganized actin filaments. Osteoblasts grown on PPy-DBS or gold showed enhanced alkaline phosphatase activity and osteocalcin gene expression, but reduced osteopontin gene expression compared to cells grown on PPy-pTS and PPy-CS. In conclusion, PPy doped with DBS showed excellent biocompatibility, which resulted in maintaining focal adhesions, cell morphology, cell number, alkaline phosphatase activity, and osteocalcin gene expression. Taken together, conducting polymers doped with DBS are well tolerated by osteoblasts. Our results could provide a basis for the development of novel orthopedic or dental implants with controlled release of antibiotics and pharmaceutics that fight infections or focally enhance bone formation in a tightly controlled manner.
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spelling pubmed-45204452015-08-06 Biocompatibility of Polypyrrole with Human Primary Osteoblasts and the Effect of Dopants Fahlgren, Anna Bratengeier, Cornelia Gelmi, Amy Semeins, Cornelis M. Klein-Nulend, Jenneke Jager, Edwin W. H. Bakker, Astrid D. PLoS One Research Article Polypyrrole (PPy) is a conducting polymer that enables controlled drug release upon electrical stimulation. We characterized the biocompatibility of PPy with human primary osteoblasts, and the effect of dopants. We investigated the biocompatibility of PPy comprising various dopants, i.e. p-toluene sulfonate (PPy-pTS), chondroitin sulfate (PPy-CS), or dodecylbenzenesulfonate (PPy-DBS), with human primary osteoblasts. PPy-DBS showed the roughest appearance of all surfaces tested, and its wettability was similar to the gold-coated control. The average number of attached cells was 45% higher on PPy-DBS than on PPy-CS or PPy-pTS, although gene expression of the proliferation marker Ki-67 was similar in osteoblasts on all surfaces tested. Osteoblasts seeded on PPy-DBS or gold showed similar vinculin attachment points, vinculin area per cell area, actin filament structure, and Feret’s diameter, while cells seeded on PPY-CS or PPY-pTS showed disturbed focal adhesions and were enlarged with disorganized actin filaments. Osteoblasts grown on PPy-DBS or gold showed enhanced alkaline phosphatase activity and osteocalcin gene expression, but reduced osteopontin gene expression compared to cells grown on PPy-pTS and PPy-CS. In conclusion, PPy doped with DBS showed excellent biocompatibility, which resulted in maintaining focal adhesions, cell morphology, cell number, alkaline phosphatase activity, and osteocalcin gene expression. Taken together, conducting polymers doped with DBS are well tolerated by osteoblasts. Our results could provide a basis for the development of novel orthopedic or dental implants with controlled release of antibiotics and pharmaceutics that fight infections or focally enhance bone formation in a tightly controlled manner. Public Library of Science 2015-07-30 /pmc/articles/PMC4520445/ /pubmed/26225862 http://dx.doi.org/10.1371/journal.pone.0134023 Text en © 2015 Fahlgren et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fahlgren, Anna
Bratengeier, Cornelia
Gelmi, Amy
Semeins, Cornelis M.
Klein-Nulend, Jenneke
Jager, Edwin W. H.
Bakker, Astrid D.
Biocompatibility of Polypyrrole with Human Primary Osteoblasts and the Effect of Dopants
title Biocompatibility of Polypyrrole with Human Primary Osteoblasts and the Effect of Dopants
title_full Biocompatibility of Polypyrrole with Human Primary Osteoblasts and the Effect of Dopants
title_fullStr Biocompatibility of Polypyrrole with Human Primary Osteoblasts and the Effect of Dopants
title_full_unstemmed Biocompatibility of Polypyrrole with Human Primary Osteoblasts and the Effect of Dopants
title_short Biocompatibility of Polypyrrole with Human Primary Osteoblasts and the Effect of Dopants
title_sort biocompatibility of polypyrrole with human primary osteoblasts and the effect of dopants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4520445/
https://www.ncbi.nlm.nih.gov/pubmed/26225862
http://dx.doi.org/10.1371/journal.pone.0134023
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