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Efficient fdCas9 Synthetic Endonuclease with Improved Specificity for Precise Genome Engineering

The Cas9 endonuclease is used for genome editing applications in diverse eukaryotic species. A high frequency of off-target activity has been reported in many cell types, limiting its applications to genome engineering, especially in genomic medicine. Here, we generated a synthetic chimeric protein...

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Detalles Bibliográficos
Autores principales:	Aouida, Mustapha, Eid, Ayman, Ali, Zahir, Cradick, Thomas, Lee, Ciaran, Deshmukh, Harshavardhan, Atef, Ahmed, AbuSamra, Dina, Gadhoum, Samah Zeineb, Merzaban, Jasmeen, Bao, Gang, Mahfouz, Magdy
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4520497/ https://www.ncbi.nlm.nih.gov/pubmed/26225561 http://dx.doi.org/10.1371/journal.pone.0133373

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