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Expression of adiponectin, chemerin and visfatin in plasma and different tissues during a laying season in turkeys

BACKGROUND: In mammals, adipose tissue is able to secrete various hormones called adipokines including adiponectin (ADP), chemerin (Chem) and visfatin (Visf) which are involved in controlling energy metabolism as well as reproductive functions. Visf receptor is still unknown whereas ADP and Chem mai...

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Autores principales: Diot, Mélodie, Reverchon, Maxime, Rame, Christelle, Froment, Pascal, Brillard, Jean-Pierre, Brière, Sylvain, Levêque, Gérard, Guillaume, Daniel, Dupont, Joëlle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521348/
https://www.ncbi.nlm.nih.gov/pubmed/26228641
http://dx.doi.org/10.1186/s12958-015-0081-5
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author Diot, Mélodie
Reverchon, Maxime
Rame, Christelle
Froment, Pascal
Brillard, Jean-Pierre
Brière, Sylvain
Levêque, Gérard
Guillaume, Daniel
Dupont, Joëlle
author_facet Diot, Mélodie
Reverchon, Maxime
Rame, Christelle
Froment, Pascal
Brillard, Jean-Pierre
Brière, Sylvain
Levêque, Gérard
Guillaume, Daniel
Dupont, Joëlle
author_sort Diot, Mélodie
collection PubMed
description BACKGROUND: In mammals, adipose tissue is able to secrete various hormones called adipokines including adiponectin (ADP), chemerin (Chem) and visfatin (Visf) which are involved in controlling energy metabolism as well as reproductive functions. Visf receptor is still unknown whereas ADP and Chem mainly act through AdipoR1, AdipoR2 and CMKLR1 and GPR1 receptors, respectively. No studies have yet demonstrated the presence of these three adipokines in peripheral tissues, ovarian cells or turkey plasma. Here, we investigated the expression (mRNA and protein) of ADP, Chem, Visf and their receptors in peripheral tissues and ovarian cells (granulosa and theca cells) from hierarchical follicles. Furthermore, we determined the plasma profile of ADP, Visf and Chem at different physiological stages: start, peak and end of the laying period in Meleagris gallopavo turkeys. This data was correlated with the metabolic data (plasma glucose, triglycerides, cholesterol and phospholipids). METHODS: Tissue and ovarian cells mRNA and protein expression levels were determined by RT-qPCR and immunoblot, respectively. Plasma adipokines were measured by chicken ELISA and immunoblotting. RESULTS: In turkeys, Chem is mainly expressed in the liver while ADP and Visf are mainly expressed in the abdominal adipose tissue and pectoral muscles,respectively. As in mammals, AdipoR1 and AdipoR2 expression levels (mRNA and protein) are highly present in muscle and liver, respectively, whereas the mRNA expression of CMKLR1 and GPR1 is ubiquitous. In ovarian cells, ADP, Visf, Chem and their receptors are more highly expressed in theca cells than in granulosa cells excepted for AdipoR1. Furthermore, we found that plasma levels of ADP, Chem and Visf were reduced at the end of the laying period compared to the start of this period. At the plasma levels, the levels of these adipokines are strongly negatively correlated with glucose and only plasma Chem is negatively correlated with cholesterol, triglycerides and phospholipids. CONCLUSIONS: In turkeys, ADP, Visf and Chem and their receptors are expressed in peripheral tissues and ovarian cells. Plasma concentration of ADP, Visf and Chem decrease at the end of laying period and only plasma Chem is negatively correlated with levels of cholesterol, triglycerides and phospholipids levels during the entire laying period.
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spelling pubmed-45213482015-08-01 Expression of adiponectin, chemerin and visfatin in plasma and different tissues during a laying season in turkeys Diot, Mélodie Reverchon, Maxime Rame, Christelle Froment, Pascal Brillard, Jean-Pierre Brière, Sylvain Levêque, Gérard Guillaume, Daniel Dupont, Joëlle Reprod Biol Endocrinol Research BACKGROUND: In mammals, adipose tissue is able to secrete various hormones called adipokines including adiponectin (ADP), chemerin (Chem) and visfatin (Visf) which are involved in controlling energy metabolism as well as reproductive functions. Visf receptor is still unknown whereas ADP and Chem mainly act through AdipoR1, AdipoR2 and CMKLR1 and GPR1 receptors, respectively. No studies have yet demonstrated the presence of these three adipokines in peripheral tissues, ovarian cells or turkey plasma. Here, we investigated the expression (mRNA and protein) of ADP, Chem, Visf and their receptors in peripheral tissues and ovarian cells (granulosa and theca cells) from hierarchical follicles. Furthermore, we determined the plasma profile of ADP, Visf and Chem at different physiological stages: start, peak and end of the laying period in Meleagris gallopavo turkeys. This data was correlated with the metabolic data (plasma glucose, triglycerides, cholesterol and phospholipids). METHODS: Tissue and ovarian cells mRNA and protein expression levels were determined by RT-qPCR and immunoblot, respectively. Plasma adipokines were measured by chicken ELISA and immunoblotting. RESULTS: In turkeys, Chem is mainly expressed in the liver while ADP and Visf are mainly expressed in the abdominal adipose tissue and pectoral muscles,respectively. As in mammals, AdipoR1 and AdipoR2 expression levels (mRNA and protein) are highly present in muscle and liver, respectively, whereas the mRNA expression of CMKLR1 and GPR1 is ubiquitous. In ovarian cells, ADP, Visf, Chem and their receptors are more highly expressed in theca cells than in granulosa cells excepted for AdipoR1. Furthermore, we found that plasma levels of ADP, Chem and Visf were reduced at the end of the laying period compared to the start of this period. At the plasma levels, the levels of these adipokines are strongly negatively correlated with glucose and only plasma Chem is negatively correlated with cholesterol, triglycerides and phospholipids. CONCLUSIONS: In turkeys, ADP, Visf and Chem and their receptors are expressed in peripheral tissues and ovarian cells. Plasma concentration of ADP, Visf and Chem decrease at the end of laying period and only plasma Chem is negatively correlated with levels of cholesterol, triglycerides and phospholipids levels during the entire laying period. BioMed Central 2015-07-31 /pmc/articles/PMC4521348/ /pubmed/26228641 http://dx.doi.org/10.1186/s12958-015-0081-5 Text en © Diot et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Diot, Mélodie
Reverchon, Maxime
Rame, Christelle
Froment, Pascal
Brillard, Jean-Pierre
Brière, Sylvain
Levêque, Gérard
Guillaume, Daniel
Dupont, Joëlle
Expression of adiponectin, chemerin and visfatin in plasma and different tissues during a laying season in turkeys
title Expression of adiponectin, chemerin and visfatin in plasma and different tissues during a laying season in turkeys
title_full Expression of adiponectin, chemerin and visfatin in plasma and different tissues during a laying season in turkeys
title_fullStr Expression of adiponectin, chemerin and visfatin in plasma and different tissues during a laying season in turkeys
title_full_unstemmed Expression of adiponectin, chemerin and visfatin in plasma and different tissues during a laying season in turkeys
title_short Expression of adiponectin, chemerin and visfatin in plasma and different tissues during a laying season in turkeys
title_sort expression of adiponectin, chemerin and visfatin in plasma and different tissues during a laying season in turkeys
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521348/
https://www.ncbi.nlm.nih.gov/pubmed/26228641
http://dx.doi.org/10.1186/s12958-015-0081-5
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