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Standardizing metadata and taxonomic identification in metabarcoding studies

High-throughput sequencing-based metabarcoding studies produce vast amounts of ecological data, but a lack of consensus on standardization of metadata and how to refer to the species recovered severely hampers reanalysis and comparisons among studies. Here we propose an automated workflow covering d...

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Detalles Bibliográficos
Autores principales: Tedersoo, Leho, Ramirez, Kelly S., Nilsson, R Henrik, Kaljuvee, Aivi, Kõljalg, Urmas, Abarenkov, Kessy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521374/
https://www.ncbi.nlm.nih.gov/pubmed/26236474
http://dx.doi.org/10.1186/s13742-015-0074-5
Descripción
Sumario:High-throughput sequencing-based metabarcoding studies produce vast amounts of ecological data, but a lack of consensus on standardization of metadata and how to refer to the species recovered severely hampers reanalysis and comparisons among studies. Here we propose an automated workflow covering data submission, compression, storage and public access to allow easy data retrieval and inter-study communication. Such standardized and readily accessible datasets facilitate data management, taxonomic comparisons and compilation of global metastudies.