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Blood-sampling collection prior to surgery may have a significant influence upon biomarker concentrations measured

BACKGROUND: Biomarkers can be subtle tools to aid the diagnosis, prognosis and monitoring of therapy and disease progression. The validation of biomarkers is a cumbersome process involving many steps. Serum samples from lung cancer patients were collected in the framework of a larger study for evalu...

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Autores principales: Kahn, Nicolas, Riedlinger, Julia, Roeßler, Markus, Rabe, Christina, Lindner, Michael, Koch, Ina, Schott-Hildebrand, Sabine, Herth, Felix J, Schneider, Marc A, Meister, Michael, Muley, Thomas R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521486/
https://www.ncbi.nlm.nih.gov/pubmed/26236175
http://dx.doi.org/10.1186/s12014-015-9093-6
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author Kahn, Nicolas
Riedlinger, Julia
Roeßler, Markus
Rabe, Christina
Lindner, Michael
Koch, Ina
Schott-Hildebrand, Sabine
Herth, Felix J
Schneider, Marc A
Meister, Michael
Muley, Thomas R
author_facet Kahn, Nicolas
Riedlinger, Julia
Roeßler, Markus
Rabe, Christina
Lindner, Michael
Koch, Ina
Schott-Hildebrand, Sabine
Herth, Felix J
Schneider, Marc A
Meister, Michael
Muley, Thomas R
author_sort Kahn, Nicolas
collection PubMed
description BACKGROUND: Biomarkers can be subtle tools to aid the diagnosis, prognosis and monitoring of therapy and disease progression. The validation of biomarkers is a cumbersome process involving many steps. Serum samples from lung cancer patients were collected in the framework of a larger study for evaluation of biomarkers for early detection of lung cancer. The analysis of biomarker levels measured revealed a noticeable difference in certain biomarker values that exhibited a dependence of the time point and setting of the sampling. Biomarker concentrations differed significantly if taken before or after the induction of anesthesia and if sampled via venipuncture or arterial catheter. METHODS: To investigate this observation, blood samples from 13 patients were drawn 1–2 days prior to surgery (T1), on the same day by venipuncture (T2) and after induction of anesthesia via arterial catheter (T3). The biomarkers Squamous Cell Carcinoma antigen (CanAG SCC EIA, Fujirebio Diagnostics, Malvern, USA), Carcinoembrionic Antigen (CEA), and CYFRA 21-1 (Roche Diagnostics GmbH, Mannheim, Germany) were analyzed. RESULTS: SCC showed a very strong effect in relation to the sampling time and procedure. While the first two points in time (T1; T2) were highly comparable (median fold-change: 0.84; p = 0.7354; correlation ρ = 0.883), patients showed a significant increase (median fold-change: 4.96; p = 0.0017; correlation ρ = -0.036) in concentration when comparing T1 with the sample time subsequent to anesthesia induction (T3). A much weaker increase was found for CYFRA 21-1 at T3 (median fold-change: 1.40; p = 0.0479). The concentration of CEA showed a very small, but systematic decrease (median fold-change: 0.72; p = 0.0039). CONCLUSIONS: In this study we show the unexpectedly marked influence of blood withdrawal timing (before vs. after anesthesia) and procedure (venous versus arterial vessel puncture) has on the concentration of the protein biomarker SCC and to a less extent upon CYFRA21-1. The potential causes for these effects remain to be elucidated in subsequent studies, however these findings highlight the importance of a standardized, controlled blood collection protocol for biomarker detection.
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spelling pubmed-45214862015-08-01 Blood-sampling collection prior to surgery may have a significant influence upon biomarker concentrations measured Kahn, Nicolas Riedlinger, Julia Roeßler, Markus Rabe, Christina Lindner, Michael Koch, Ina Schott-Hildebrand, Sabine Herth, Felix J Schneider, Marc A Meister, Michael Muley, Thomas R Clin Proteomics Research BACKGROUND: Biomarkers can be subtle tools to aid the diagnosis, prognosis and monitoring of therapy and disease progression. The validation of biomarkers is a cumbersome process involving many steps. Serum samples from lung cancer patients were collected in the framework of a larger study for evaluation of biomarkers for early detection of lung cancer. The analysis of biomarker levels measured revealed a noticeable difference in certain biomarker values that exhibited a dependence of the time point and setting of the sampling. Biomarker concentrations differed significantly if taken before or after the induction of anesthesia and if sampled via venipuncture or arterial catheter. METHODS: To investigate this observation, blood samples from 13 patients were drawn 1–2 days prior to surgery (T1), on the same day by venipuncture (T2) and after induction of anesthesia via arterial catheter (T3). The biomarkers Squamous Cell Carcinoma antigen (CanAG SCC EIA, Fujirebio Diagnostics, Malvern, USA), Carcinoembrionic Antigen (CEA), and CYFRA 21-1 (Roche Diagnostics GmbH, Mannheim, Germany) were analyzed. RESULTS: SCC showed a very strong effect in relation to the sampling time and procedure. While the first two points in time (T1; T2) were highly comparable (median fold-change: 0.84; p = 0.7354; correlation ρ = 0.883), patients showed a significant increase (median fold-change: 4.96; p = 0.0017; correlation ρ = -0.036) in concentration when comparing T1 with the sample time subsequent to anesthesia induction (T3). A much weaker increase was found for CYFRA 21-1 at T3 (median fold-change: 1.40; p = 0.0479). The concentration of CEA showed a very small, but systematic decrease (median fold-change: 0.72; p = 0.0039). CONCLUSIONS: In this study we show the unexpectedly marked influence of blood withdrawal timing (before vs. after anesthesia) and procedure (venous versus arterial vessel puncture) has on the concentration of the protein biomarker SCC and to a less extent upon CYFRA21-1. The potential causes for these effects remain to be elucidated in subsequent studies, however these findings highlight the importance of a standardized, controlled blood collection protocol for biomarker detection. BioMed Central 2015-07-31 /pmc/articles/PMC4521486/ /pubmed/26236175 http://dx.doi.org/10.1186/s12014-015-9093-6 Text en © Kahn et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Kahn, Nicolas
Riedlinger, Julia
Roeßler, Markus
Rabe, Christina
Lindner, Michael
Koch, Ina
Schott-Hildebrand, Sabine
Herth, Felix J
Schneider, Marc A
Meister, Michael
Muley, Thomas R
Blood-sampling collection prior to surgery may have a significant influence upon biomarker concentrations measured
title Blood-sampling collection prior to surgery may have a significant influence upon biomarker concentrations measured
title_full Blood-sampling collection prior to surgery may have a significant influence upon biomarker concentrations measured
title_fullStr Blood-sampling collection prior to surgery may have a significant influence upon biomarker concentrations measured
title_full_unstemmed Blood-sampling collection prior to surgery may have a significant influence upon biomarker concentrations measured
title_short Blood-sampling collection prior to surgery may have a significant influence upon biomarker concentrations measured
title_sort blood-sampling collection prior to surgery may have a significant influence upon biomarker concentrations measured
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521486/
https://www.ncbi.nlm.nih.gov/pubmed/26236175
http://dx.doi.org/10.1186/s12014-015-9093-6
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