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The Ultrastructural Localization of Type II, IV, and VI Collagens at the Vitreoretinal Interface

BACKGROUND: The vitreoretinal interface is the border of the cortical vitreous and the inner surface of the retina. The adhesion of the cortical vitreous to the ILM, namely vitreoretinal adhesion, involves a series of complex molecular adhesion mechanisms and has been considered as an important path...

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Autores principales: Bu, Shao Chong, Kuijer, Roel, van der Worp, Roelofje J., Li, Xiao Rong, Hooymans, Johanna M. M., Los, Leonoor I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521792/
https://www.ncbi.nlm.nih.gov/pubmed/26230410
http://dx.doi.org/10.1371/journal.pone.0134325
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author Bu, Shao Chong
Kuijer, Roel
van der Worp, Roelofje J.
Li, Xiao Rong
Hooymans, Johanna M. M.
Los, Leonoor I.
author_facet Bu, Shao Chong
Kuijer, Roel
van der Worp, Roelofje J.
Li, Xiao Rong
Hooymans, Johanna M. M.
Los, Leonoor I.
author_sort Bu, Shao Chong
collection PubMed
description BACKGROUND: The vitreoretinal interface is the border of the cortical vitreous and the inner surface of the retina. The adhesion of the cortical vitreous to the ILM, namely vitreoretinal adhesion, involves a series of complex molecular adhesion mechanisms and has been considered as an important pathogenic factor in many vitreoretinal diseases. The presence of type VI collagen at the vitreoretinal interface and its possible interaction with collagens and glycoproteins indicates that type VI collagen may contribute to the vitreoretinal adhesion. PURPOSE: To clarify the ultrastructural location of type VI collagen and its relationship to type II and IV collagens at the vitreoretinal interface. METHODS: The ultrastructural localization of type II, IV and VI collagens in the adult human vitreoretinal interface of five donor eyes was evaluated by transmission electron microscopy using immunogold labeling. RESULTS: In the pre-equatorial region, we observed densely packed vitreous lamellae with a partly intraretinal course containing type II and VI collagens, reticular structures containing type IV and VI collagens and a thin inner limiting membrane (ILM) containing type IV and VI collagens in a linear distribution pattern. From the anterior to the posterior retina, the linear pattern of type IV and VI collagen labeling gradually became more diffusely present throughout the entire thickness of the ILM. CONCLUSIONS: The presence of type VI collagen in vitreous lamellae penetrating the ILM into the superficial retina suggests that type VI collagen may be involved in the organization of vitreous fibers into lamellae and in the adhesion of the vitreous fibers to the retina. The close relation of type VI to type IV collagen in the ILM suggests that type VI collagen is an important collagen type in the ILM. The topographic variations of type IV and VI collagens in the different regions of the ILM suggest a regional heterogeneity of the ILM. The reticular labeling pattern of type IV and VI collagens observed in the anterior vitreous are highly similar to labeling patterns of blood vessel walls. In the anterior vitreous, they may represent remnants of the regressed embryonic hyaloid blood vessel system. Their presence is in support of the theory on interactive remodeling of the developing vitreous as opposed to the main stream theory of displacement and compression of the primary by the secondary vitreous.
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spelling pubmed-45217922015-08-06 The Ultrastructural Localization of Type II, IV, and VI Collagens at the Vitreoretinal Interface Bu, Shao Chong Kuijer, Roel van der Worp, Roelofje J. Li, Xiao Rong Hooymans, Johanna M. M. Los, Leonoor I. PLoS One Research Article BACKGROUND: The vitreoretinal interface is the border of the cortical vitreous and the inner surface of the retina. The adhesion of the cortical vitreous to the ILM, namely vitreoretinal adhesion, involves a series of complex molecular adhesion mechanisms and has been considered as an important pathogenic factor in many vitreoretinal diseases. The presence of type VI collagen at the vitreoretinal interface and its possible interaction with collagens and glycoproteins indicates that type VI collagen may contribute to the vitreoretinal adhesion. PURPOSE: To clarify the ultrastructural location of type VI collagen and its relationship to type II and IV collagens at the vitreoretinal interface. METHODS: The ultrastructural localization of type II, IV and VI collagens in the adult human vitreoretinal interface of five donor eyes was evaluated by transmission electron microscopy using immunogold labeling. RESULTS: In the pre-equatorial region, we observed densely packed vitreous lamellae with a partly intraretinal course containing type II and VI collagens, reticular structures containing type IV and VI collagens and a thin inner limiting membrane (ILM) containing type IV and VI collagens in a linear distribution pattern. From the anterior to the posterior retina, the linear pattern of type IV and VI collagen labeling gradually became more diffusely present throughout the entire thickness of the ILM. CONCLUSIONS: The presence of type VI collagen in vitreous lamellae penetrating the ILM into the superficial retina suggests that type VI collagen may be involved in the organization of vitreous fibers into lamellae and in the adhesion of the vitreous fibers to the retina. The close relation of type VI to type IV collagen in the ILM suggests that type VI collagen is an important collagen type in the ILM. The topographic variations of type IV and VI collagens in the different regions of the ILM suggest a regional heterogeneity of the ILM. The reticular labeling pattern of type IV and VI collagens observed in the anterior vitreous are highly similar to labeling patterns of blood vessel walls. In the anterior vitreous, they may represent remnants of the regressed embryonic hyaloid blood vessel system. Their presence is in support of the theory on interactive remodeling of the developing vitreous as opposed to the main stream theory of displacement and compression of the primary by the secondary vitreous. Public Library of Science 2015-07-31 /pmc/articles/PMC4521792/ /pubmed/26230410 http://dx.doi.org/10.1371/journal.pone.0134325 Text en © 2015 Bu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bu, Shao Chong
Kuijer, Roel
van der Worp, Roelofje J.
Li, Xiao Rong
Hooymans, Johanna M. M.
Los, Leonoor I.
The Ultrastructural Localization of Type II, IV, and VI Collagens at the Vitreoretinal Interface
title The Ultrastructural Localization of Type II, IV, and VI Collagens at the Vitreoretinal Interface
title_full The Ultrastructural Localization of Type II, IV, and VI Collagens at the Vitreoretinal Interface
title_fullStr The Ultrastructural Localization of Type II, IV, and VI Collagens at the Vitreoretinal Interface
title_full_unstemmed The Ultrastructural Localization of Type II, IV, and VI Collagens at the Vitreoretinal Interface
title_short The Ultrastructural Localization of Type II, IV, and VI Collagens at the Vitreoretinal Interface
title_sort ultrastructural localization of type ii, iv, and vi collagens at the vitreoretinal interface
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521792/
https://www.ncbi.nlm.nih.gov/pubmed/26230410
http://dx.doi.org/10.1371/journal.pone.0134325
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