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Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions

Epicatechin (EC), epigallocatechin (EGC), epicatechingallate (ECG) and epigallocatechingallate (EGCG) are antioxidants present in the green tea, a widely used beverage whose health benefits are largely recognized. Nevertheless, major physicochemical limitations, such as the high instability of catec...

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Autores principales: Zinellu, Angelo, Sotgia, Salvatore, Scanu, Bastianina, Forteschi, Mauro, Giordo, Roberta, Cossu, Annalisa, Posadino, Anna Maria, Carru, Ciriaco, Pintus, Gianfranco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521934/
https://www.ncbi.nlm.nih.gov/pubmed/26230943
http://dx.doi.org/10.1371/journal.pone.0134690
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author Zinellu, Angelo
Sotgia, Salvatore
Scanu, Bastianina
Forteschi, Mauro
Giordo, Roberta
Cossu, Annalisa
Posadino, Anna Maria
Carru, Ciriaco
Pintus, Gianfranco
author_facet Zinellu, Angelo
Sotgia, Salvatore
Scanu, Bastianina
Forteschi, Mauro
Giordo, Roberta
Cossu, Annalisa
Posadino, Anna Maria
Carru, Ciriaco
Pintus, Gianfranco
author_sort Zinellu, Angelo
collection PubMed
description Epicatechin (EC), epigallocatechin (EGC), epicatechingallate (ECG) and epigallocatechingallate (EGCG) are antioxidants present in the green tea, a widely used beverage whose health benefits are largely recognized. Nevertheless, major physicochemical limitations, such as the high instability of catechins, pose important questions concerning their potential pharmacological use. Recent studies indicate that binding of catechins with plasmatic proteins may modulate their plasma concentration, tissue delivery and biological activity. After 5 minutes of incubation with HSA both ECG and EGCG were fully bound to HSA, while after 48h incubation only 41% of EC and 70% of EGC resulted linked. HSA had a strong stabilizing effect on all catechins, which could be found in solution between 29 and 85% even after 48h of incubation. In the absence of HSA, EGC and EGCG disappeared in less than 24h, while ECG and EC were found after 48h at 5 and 50%, respectively. The stabilizing effect of HSA toward EGCG, obtained in aqueous physiological conditions, resulted stronger in comparison to cysteine and HCl, previously reported to stabilize this polyphenol. Because of the multitude of contradictory data concerning in vivo and in vitro antioxidant-based experimentations, we believe our work may shed some light on this debated field of research.
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spelling pubmed-45219342015-08-06 Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions Zinellu, Angelo Sotgia, Salvatore Scanu, Bastianina Forteschi, Mauro Giordo, Roberta Cossu, Annalisa Posadino, Anna Maria Carru, Ciriaco Pintus, Gianfranco PLoS One Research Article Epicatechin (EC), epigallocatechin (EGC), epicatechingallate (ECG) and epigallocatechingallate (EGCG) are antioxidants present in the green tea, a widely used beverage whose health benefits are largely recognized. Nevertheless, major physicochemical limitations, such as the high instability of catechins, pose important questions concerning their potential pharmacological use. Recent studies indicate that binding of catechins with plasmatic proteins may modulate their plasma concentration, tissue delivery and biological activity. After 5 minutes of incubation with HSA both ECG and EGCG were fully bound to HSA, while after 48h incubation only 41% of EC and 70% of EGC resulted linked. HSA had a strong stabilizing effect on all catechins, which could be found in solution between 29 and 85% even after 48h of incubation. In the absence of HSA, EGC and EGCG disappeared in less than 24h, while ECG and EC were found after 48h at 5 and 50%, respectively. The stabilizing effect of HSA toward EGCG, obtained in aqueous physiological conditions, resulted stronger in comparison to cysteine and HCl, previously reported to stabilize this polyphenol. Because of the multitude of contradictory data concerning in vivo and in vitro antioxidant-based experimentations, we believe our work may shed some light on this debated field of research. Public Library of Science 2015-07-31 /pmc/articles/PMC4521934/ /pubmed/26230943 http://dx.doi.org/10.1371/journal.pone.0134690 Text en © 2015 Zinellu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zinellu, Angelo
Sotgia, Salvatore
Scanu, Bastianina
Forteschi, Mauro
Giordo, Roberta
Cossu, Annalisa
Posadino, Anna Maria
Carru, Ciriaco
Pintus, Gianfranco
Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions
title Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions
title_full Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions
title_fullStr Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions
title_full_unstemmed Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions
title_short Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions
title_sort human serum albumin increases the stability of green tea catechins in aqueous physiological conditions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521934/
https://www.ncbi.nlm.nih.gov/pubmed/26230943
http://dx.doi.org/10.1371/journal.pone.0134690
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