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Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions
Epicatechin (EC), epigallocatechin (EGC), epicatechingallate (ECG) and epigallocatechingallate (EGCG) are antioxidants present in the green tea, a widely used beverage whose health benefits are largely recognized. Nevertheless, major physicochemical limitations, such as the high instability of catec...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521934/ https://www.ncbi.nlm.nih.gov/pubmed/26230943 http://dx.doi.org/10.1371/journal.pone.0134690 |
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author | Zinellu, Angelo Sotgia, Salvatore Scanu, Bastianina Forteschi, Mauro Giordo, Roberta Cossu, Annalisa Posadino, Anna Maria Carru, Ciriaco Pintus, Gianfranco |
author_facet | Zinellu, Angelo Sotgia, Salvatore Scanu, Bastianina Forteschi, Mauro Giordo, Roberta Cossu, Annalisa Posadino, Anna Maria Carru, Ciriaco Pintus, Gianfranco |
author_sort | Zinellu, Angelo |
collection | PubMed |
description | Epicatechin (EC), epigallocatechin (EGC), epicatechingallate (ECG) and epigallocatechingallate (EGCG) are antioxidants present in the green tea, a widely used beverage whose health benefits are largely recognized. Nevertheless, major physicochemical limitations, such as the high instability of catechins, pose important questions concerning their potential pharmacological use. Recent studies indicate that binding of catechins with plasmatic proteins may modulate their plasma concentration, tissue delivery and biological activity. After 5 minutes of incubation with HSA both ECG and EGCG were fully bound to HSA, while after 48h incubation only 41% of EC and 70% of EGC resulted linked. HSA had a strong stabilizing effect on all catechins, which could be found in solution between 29 and 85% even after 48h of incubation. In the absence of HSA, EGC and EGCG disappeared in less than 24h, while ECG and EC were found after 48h at 5 and 50%, respectively. The stabilizing effect of HSA toward EGCG, obtained in aqueous physiological conditions, resulted stronger in comparison to cysteine and HCl, previously reported to stabilize this polyphenol. Because of the multitude of contradictory data concerning in vivo and in vitro antioxidant-based experimentations, we believe our work may shed some light on this debated field of research. |
format | Online Article Text |
id | pubmed-4521934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45219342015-08-06 Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions Zinellu, Angelo Sotgia, Salvatore Scanu, Bastianina Forteschi, Mauro Giordo, Roberta Cossu, Annalisa Posadino, Anna Maria Carru, Ciriaco Pintus, Gianfranco PLoS One Research Article Epicatechin (EC), epigallocatechin (EGC), epicatechingallate (ECG) and epigallocatechingallate (EGCG) are antioxidants present in the green tea, a widely used beverage whose health benefits are largely recognized. Nevertheless, major physicochemical limitations, such as the high instability of catechins, pose important questions concerning their potential pharmacological use. Recent studies indicate that binding of catechins with plasmatic proteins may modulate their plasma concentration, tissue delivery and biological activity. After 5 minutes of incubation with HSA both ECG and EGCG were fully bound to HSA, while after 48h incubation only 41% of EC and 70% of EGC resulted linked. HSA had a strong stabilizing effect on all catechins, which could be found in solution between 29 and 85% even after 48h of incubation. In the absence of HSA, EGC and EGCG disappeared in less than 24h, while ECG and EC were found after 48h at 5 and 50%, respectively. The stabilizing effect of HSA toward EGCG, obtained in aqueous physiological conditions, resulted stronger in comparison to cysteine and HCl, previously reported to stabilize this polyphenol. Because of the multitude of contradictory data concerning in vivo and in vitro antioxidant-based experimentations, we believe our work may shed some light on this debated field of research. Public Library of Science 2015-07-31 /pmc/articles/PMC4521934/ /pubmed/26230943 http://dx.doi.org/10.1371/journal.pone.0134690 Text en © 2015 Zinellu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zinellu, Angelo Sotgia, Salvatore Scanu, Bastianina Forteschi, Mauro Giordo, Roberta Cossu, Annalisa Posadino, Anna Maria Carru, Ciriaco Pintus, Gianfranco Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions |
title | Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions |
title_full | Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions |
title_fullStr | Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions |
title_full_unstemmed | Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions |
title_short | Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions |
title_sort | human serum albumin increases the stability of green tea catechins in aqueous physiological conditions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521934/ https://www.ncbi.nlm.nih.gov/pubmed/26230943 http://dx.doi.org/10.1371/journal.pone.0134690 |
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