Single cell magnetic imaging using a quantum diamond microscope

We apply a quantum diamond microscope to detection and imaging of immunomagnetically labeled cells. This instrument uses nitrogen-vacancy (NV) centers in diamond for correlated magnetic and fluorescence imaging. Our device provides single-cell resolution and two orders of magnitude larger field of v...

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Detalles Bibliográficos
Autores principales: Glenn, D. R., Lee, K., Park, H., Weissleder, R., Yacoby, A., Lukin, M. D., Lee, H., Walsworth, R. L., Connolly, C. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521973/
https://www.ncbi.nlm.nih.gov/pubmed/26098019
http://dx.doi.org/10.1038/nmeth.3449
Descripción
Sumario:We apply a quantum diamond microscope to detection and imaging of immunomagnetically labeled cells. This instrument uses nitrogen-vacancy (NV) centers in diamond for correlated magnetic and fluorescence imaging. Our device provides single-cell resolution and two orders of magnitude larger field of view (~1 mm(2)) than previous NV imaging technologies, enabling practical applications. To illustrate, we quantify cancer biomarkers expressed by rare tumor cells in a large population of healthy cells.

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