COMBINING PROTEIN AND mRNA QUANTIFICATION TO DECIPHER TRANSCRIPTIONAL REGULATION

We combine immunofluorescence and single-molecule fluorescence in situ hybridization (smFISH), followed by automated image analysis, to quantify the concentration of nuclear transcription factors, number of transcription factors bound, and number of nascent mRNAs synthesized at individual gene loci....

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Detalles Bibliográficos
Autores principales: Xu, Heng, Sepúlveda, Leonardo A., Figard, Lauren, Sokac, Anna Marie, Golding, Ido
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521975/
https://www.ncbi.nlm.nih.gov/pubmed/26098021
http://dx.doi.org/10.1038/nmeth.3446

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521975/
https://www.ncbi.nlm.nih.gov/pubmed/26098021
http://dx.doi.org/10.1038/nmeth.3446

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