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A naturally-monomeric infrared fluorescent protein for protein labeling in vivo
Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its red homologs in protein labeling. Based on structural analysis of the dimer interface, a monomeric bateriophytochrome is identified from a sequence database, and is engineered into a naturally-monomeric IFP (mIFP). We de...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521985/ https://www.ncbi.nlm.nih.gov/pubmed/26098020 http://dx.doi.org/10.1038/nmeth.3447 |
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| author | Yu, Dan Baird, Michelle A. Allen, John R. Howe, Elizabeth S. Klassen, Matthew P. Reade, Anna Makhijani, Kalpana Song, Yuanquan Liu, Songmei Murthy, Zehra Zhang, Shao-Qing Weiner, Orion D. Kornberg, Thomas B. Jan, Yuh-Nung Davidson, Michael W. Shu, Xiaokun |
| author_facet | Yu, Dan Baird, Michelle A. Allen, John R. Howe, Elizabeth S. Klassen, Matthew P. Reade, Anna Makhijani, Kalpana Song, Yuanquan Liu, Songmei Murthy, Zehra Zhang, Shao-Qing Weiner, Orion D. Kornberg, Thomas B. Jan, Yuh-Nung Davidson, Michael W. Shu, Xiaokun |
| author_sort | Yu, Dan |
| collection | PubMed |
| description | Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its red homologs in protein labeling. Based on structural analysis of the dimer interface, a monomeric bateriophytochrome is identified from a sequence database, and is engineered into a naturally-monomeric IFP (mIFP). We demonstrate that mIFP correctly labels proteins in live Drosophila and zebrafish requiring no exogenous cofactor, and will thus be useful in molecular, cell and developmental biology. |
| format | Online Article Text |
| id | pubmed-4521985 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2015 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-45219852016-01-31 A naturally-monomeric infrared fluorescent protein for protein labeling in vivo Yu, Dan Baird, Michelle A. Allen, John R. Howe, Elizabeth S. Klassen, Matthew P. Reade, Anna Makhijani, Kalpana Song, Yuanquan Liu, Songmei Murthy, Zehra Zhang, Shao-Qing Weiner, Orion D. Kornberg, Thomas B. Jan, Yuh-Nung Davidson, Michael W. Shu, Xiaokun Nat Methods Article Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its red homologs in protein labeling. Based on structural analysis of the dimer interface, a monomeric bateriophytochrome is identified from a sequence database, and is engineered into a naturally-monomeric IFP (mIFP). We demonstrate that mIFP correctly labels proteins in live Drosophila and zebrafish requiring no exogenous cofactor, and will thus be useful in molecular, cell and developmental biology. 2015-06-22 2015-08 /pmc/articles/PMC4521985/ /pubmed/26098020 http://dx.doi.org/10.1038/nmeth.3447 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Yu, Dan Baird, Michelle A. Allen, John R. Howe, Elizabeth S. Klassen, Matthew P. Reade, Anna Makhijani, Kalpana Song, Yuanquan Liu, Songmei Murthy, Zehra Zhang, Shao-Qing Weiner, Orion D. Kornberg, Thomas B. Jan, Yuh-Nung Davidson, Michael W. Shu, Xiaokun A naturally-monomeric infrared fluorescent protein for protein labeling in vivo |
| title | A naturally-monomeric infrared fluorescent protein for protein labeling in vivo |
| title_full | A naturally-monomeric infrared fluorescent protein for protein labeling in vivo |
| title_fullStr | A naturally-monomeric infrared fluorescent protein for protein labeling in vivo |
| title_full_unstemmed | A naturally-monomeric infrared fluorescent protein for protein labeling in vivo |
| title_short | A naturally-monomeric infrared fluorescent protein for protein labeling in vivo |
| title_sort | naturally-monomeric infrared fluorescent protein for protein labeling in vivo |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4521985/ https://www.ncbi.nlm.nih.gov/pubmed/26098020 http://dx.doi.org/10.1038/nmeth.3447 |
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