Cargando…

The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis

The present study was conducted to characterize the polyphenolic contents of lettuce leaves grown under different night-time temperatures (4, 12, and 20 °C) and cultivation durations (5, 15, and 20 days) using high performance liquid chromatography-tandem mass spectrometry (LC/MS/MS). The assay meth...

Descripción completa

Detalles Bibliográficos
Autores principales: Jeong, Sung Woo, Kim, Gon-Sup, Lee, Won Sup, Kim, Yun-Hi, Kang, Nam Jun, Jin, Jong Sung, Lee, Gye Min, Kim, Soo Taek, Abd El-Aty, A.M., Shim, Jae-Han, Shin, Sung Chul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522575/
https://www.ncbi.nlm.nih.gov/pubmed/26257947
http://dx.doi.org/10.1016/j.jare.2015.01.004
Descripción
Sumario:The present study was conducted to characterize the polyphenolic contents of lettuce leaves grown under different night-time temperatures (4, 12, and 20 °C) and cultivation durations (5, 15, and 20 days) using high performance liquid chromatography-tandem mass spectrometry (LC/MS/MS). The assay method was validated based on specificity, linearity, accuracy, precision, and the performance limit. The total polyphenolic contents were highest (2462.6 mg/kg) after transplantation at a night temperature of 20 °C on day 20 and lowest (1132.7 mg/kg) at the same temperature on day 5. Quantification and principal component analysis showed that the relative contents of quercetin and kaempferol were markedly higher during the early stage of cultivation (day 5) than those of day 15 and 20, and that night-time temperatures of 12 and 20 °C on day 20 were favorable for producing polyphenol-rich lettuce containing caffeic acid. In conclusion, a synergistic effect between high night-time temperatures (12 and 20 °C) and cultivation duration (20 days) produced lettuce rich in polyphenols compared to that at low temperature (4 °C).