Cargando…
The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis
The present study was conducted to characterize the polyphenolic contents of lettuce leaves grown under different night-time temperatures (4, 12, and 20 °C) and cultivation durations (5, 15, and 20 days) using high performance liquid chromatography-tandem mass spectrometry (LC/MS/MS). The assay meth...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522575/ https://www.ncbi.nlm.nih.gov/pubmed/26257947 http://dx.doi.org/10.1016/j.jare.2015.01.004 |
_version_ | 1782383969710374912 |
---|---|
author | Jeong, Sung Woo Kim, Gon-Sup Lee, Won Sup Kim, Yun-Hi Kang, Nam Jun Jin, Jong Sung Lee, Gye Min Kim, Soo Taek Abd El-Aty, A.M. Shim, Jae-Han Shin, Sung Chul |
author_facet | Jeong, Sung Woo Kim, Gon-Sup Lee, Won Sup Kim, Yun-Hi Kang, Nam Jun Jin, Jong Sung Lee, Gye Min Kim, Soo Taek Abd El-Aty, A.M. Shim, Jae-Han Shin, Sung Chul |
author_sort | Jeong, Sung Woo |
collection | PubMed |
description | The present study was conducted to characterize the polyphenolic contents of lettuce leaves grown under different night-time temperatures (4, 12, and 20 °C) and cultivation durations (5, 15, and 20 days) using high performance liquid chromatography-tandem mass spectrometry (LC/MS/MS). The assay method was validated based on specificity, linearity, accuracy, precision, and the performance limit. The total polyphenolic contents were highest (2462.6 mg/kg) after transplantation at a night temperature of 20 °C on day 20 and lowest (1132.7 mg/kg) at the same temperature on day 5. Quantification and principal component analysis showed that the relative contents of quercetin and kaempferol were markedly higher during the early stage of cultivation (day 5) than those of day 15 and 20, and that night-time temperatures of 12 and 20 °C on day 20 were favorable for producing polyphenol-rich lettuce containing caffeic acid. In conclusion, a synergistic effect between high night-time temperatures (12 and 20 °C) and cultivation duration (20 days) produced lettuce rich in polyphenols compared to that at low temperature (4 °C). |
format | Online Article Text |
id | pubmed-4522575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-45225752015-08-07 The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis Jeong, Sung Woo Kim, Gon-Sup Lee, Won Sup Kim, Yun-Hi Kang, Nam Jun Jin, Jong Sung Lee, Gye Min Kim, Soo Taek Abd El-Aty, A.M. Shim, Jae-Han Shin, Sung Chul J Adv Res Original Article The present study was conducted to characterize the polyphenolic contents of lettuce leaves grown under different night-time temperatures (4, 12, and 20 °C) and cultivation durations (5, 15, and 20 days) using high performance liquid chromatography-tandem mass spectrometry (LC/MS/MS). The assay method was validated based on specificity, linearity, accuracy, precision, and the performance limit. The total polyphenolic contents were highest (2462.6 mg/kg) after transplantation at a night temperature of 20 °C on day 20 and lowest (1132.7 mg/kg) at the same temperature on day 5. Quantification and principal component analysis showed that the relative contents of quercetin and kaempferol were markedly higher during the early stage of cultivation (day 5) than those of day 15 and 20, and that night-time temperatures of 12 and 20 °C on day 20 were favorable for producing polyphenol-rich lettuce containing caffeic acid. In conclusion, a synergistic effect between high night-time temperatures (12 and 20 °C) and cultivation duration (20 days) produced lettuce rich in polyphenols compared to that at low temperature (4 °C). Elsevier 2015-05 2015-01-21 /pmc/articles/PMC4522575/ /pubmed/26257947 http://dx.doi.org/10.1016/j.jare.2015.01.004 Text en © 2015 Production and hosting by Elsevier B.V. on behalf of Cairo University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Jeong, Sung Woo Kim, Gon-Sup Lee, Won Sup Kim, Yun-Hi Kang, Nam Jun Jin, Jong Sung Lee, Gye Min Kim, Soo Taek Abd El-Aty, A.M. Shim, Jae-Han Shin, Sung Chul The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis |
title | The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis |
title_full | The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis |
title_fullStr | The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis |
title_full_unstemmed | The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis |
title_short | The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis |
title_sort | effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: application of principal component analysis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522575/ https://www.ncbi.nlm.nih.gov/pubmed/26257947 http://dx.doi.org/10.1016/j.jare.2015.01.004 |
work_keys_str_mv | AT jeongsungwoo theeffectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT kimgonsup theeffectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT leewonsup theeffectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT kimyunhi theeffectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT kangnamjun theeffectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT jinjongsung theeffectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT leegyemin theeffectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT kimsootaek theeffectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT abdelatyam theeffectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT shimjaehan theeffectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT shinsungchul theeffectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT jeongsungwoo effectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT kimgonsup effectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT leewonsup effectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT kimyunhi effectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT kangnamjun effectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT jinjongsung effectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT leegyemin effectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT kimsootaek effectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT abdelatyam effectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT shimjaehan effectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis AT shinsungchul effectsofdifferentnighttimetemperaturesandcultivationdurationsonthepolyphenoliccontentsoflettuceapplicationofprincipalcomponentanalysis |