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Early diagnosis of bladder cancer through the detection of urinary tyrosine-phosphorylated proteins

BACKGROUND: A noninvasive, highly sensitive and specific urine test is needed for bladder cancer (BC) diagnosis and surveillance in addition to the invasive cystoscopy. We previously described the diagnostic effectiveness of urinary tyrosine-phosphorylated proteins (UPY) and a new assay (UPY-A) for...

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Autores principales: Khadjavi, A, Mannu, F, Destefanis, P, Sacerdote, C, Battaglia, A, Allasia, M, Fontana, D, Frea, B, Polidoro, S, Fiorito, G, Matullo, G, Pantaleo, A, Notarpietro, A, Prato, M, Castagno, F, Vineis, P, Gontero, P, Giribaldi, G, Turrini, F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522638/
https://www.ncbi.nlm.nih.gov/pubmed/26125446
http://dx.doi.org/10.1038/bjc.2015.232
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author Khadjavi, A
Mannu, F
Destefanis, P
Sacerdote, C
Battaglia, A
Allasia, M
Fontana, D
Frea, B
Polidoro, S
Fiorito, G
Matullo, G
Pantaleo, A
Notarpietro, A
Prato, M
Castagno, F
Vineis, P
Gontero, P
Giribaldi, G
Turrini, F
author_facet Khadjavi, A
Mannu, F
Destefanis, P
Sacerdote, C
Battaglia, A
Allasia, M
Fontana, D
Frea, B
Polidoro, S
Fiorito, G
Matullo, G
Pantaleo, A
Notarpietro, A
Prato, M
Castagno, F
Vineis, P
Gontero, P
Giribaldi, G
Turrini, F
author_sort Khadjavi, A
collection PubMed
description BACKGROUND: A noninvasive, highly sensitive and specific urine test is needed for bladder cancer (BC) diagnosis and surveillance in addition to the invasive cystoscopy. We previously described the diagnostic effectiveness of urinary tyrosine-phosphorylated proteins (UPY) and a new assay (UPY-A) for their measurement in a pilot study. The aim of this work was to evaluate the performances of the UPY-A using an independent cohort of 262 subjects. METHODS: Urinary tyrosine-phosphorylated proteins were measured by UPY-A test. The area under ROC curve, cutoff, sensitivity, specificity and predictive values of UPY-A were determined. The association of UPY levels with tumour staging, grading, recurrence and progression risk was analysed by Kruskal–Wallis and Wilcoxon's test. To test the probability to be a case if positive at the UPY-A, a logistic test adjusted for possible confounding factor was used. RESULTS: Results showed a significant difference of UPY levels between patients with BC vs healthy controls. For the best cutoff value, 261.26 Standard Units (SU), the sensitivity of the assay was 80.43% and the specificity was 78.82%. A statistically significant difference was found in the levels of UPY at different BC stages and grades between Ta and T1 and with different risk of recurrence and progression. A statistically significant increased risk for BC at UPY-A ⩾261.26 SU was observed. CONCLUSIONS: The present study supplies important information on the diagnostic characteristics of UPY-A revealing remarkable performances for early stages and allowing its potential use for different applications encompassing the screening of high-risk subjects, primary diagnosis and posttreatment surveillance.
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spelling pubmed-45226382016-07-28 Early diagnosis of bladder cancer through the detection of urinary tyrosine-phosphorylated proteins Khadjavi, A Mannu, F Destefanis, P Sacerdote, C Battaglia, A Allasia, M Fontana, D Frea, B Polidoro, S Fiorito, G Matullo, G Pantaleo, A Notarpietro, A Prato, M Castagno, F Vineis, P Gontero, P Giribaldi, G Turrini, F Br J Cancer Molecular Diagnostics BACKGROUND: A noninvasive, highly sensitive and specific urine test is needed for bladder cancer (BC) diagnosis and surveillance in addition to the invasive cystoscopy. We previously described the diagnostic effectiveness of urinary tyrosine-phosphorylated proteins (UPY) and a new assay (UPY-A) for their measurement in a pilot study. The aim of this work was to evaluate the performances of the UPY-A using an independent cohort of 262 subjects. METHODS: Urinary tyrosine-phosphorylated proteins were measured by UPY-A test. The area under ROC curve, cutoff, sensitivity, specificity and predictive values of UPY-A were determined. The association of UPY levels with tumour staging, grading, recurrence and progression risk was analysed by Kruskal–Wallis and Wilcoxon's test. To test the probability to be a case if positive at the UPY-A, a logistic test adjusted for possible confounding factor was used. RESULTS: Results showed a significant difference of UPY levels between patients with BC vs healthy controls. For the best cutoff value, 261.26 Standard Units (SU), the sensitivity of the assay was 80.43% and the specificity was 78.82%. A statistically significant difference was found in the levels of UPY at different BC stages and grades between Ta and T1 and with different risk of recurrence and progression. A statistically significant increased risk for BC at UPY-A ⩾261.26 SU was observed. CONCLUSIONS: The present study supplies important information on the diagnostic characteristics of UPY-A revealing remarkable performances for early stages and allowing its potential use for different applications encompassing the screening of high-risk subjects, primary diagnosis and posttreatment surveillance. Nature Publishing Group 2015-07-28 2015-06-30 /pmc/articles/PMC4522638/ /pubmed/26125446 http://dx.doi.org/10.1038/bjc.2015.232 Text en Copyright © 2015 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/4.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Molecular Diagnostics
Khadjavi, A
Mannu, F
Destefanis, P
Sacerdote, C
Battaglia, A
Allasia, M
Fontana, D
Frea, B
Polidoro, S
Fiorito, G
Matullo, G
Pantaleo, A
Notarpietro, A
Prato, M
Castagno, F
Vineis, P
Gontero, P
Giribaldi, G
Turrini, F
Early diagnosis of bladder cancer through the detection of urinary tyrosine-phosphorylated proteins
title Early diagnosis of bladder cancer through the detection of urinary tyrosine-phosphorylated proteins
title_full Early diagnosis of bladder cancer through the detection of urinary tyrosine-phosphorylated proteins
title_fullStr Early diagnosis of bladder cancer through the detection of urinary tyrosine-phosphorylated proteins
title_full_unstemmed Early diagnosis of bladder cancer through the detection of urinary tyrosine-phosphorylated proteins
title_short Early diagnosis of bladder cancer through the detection of urinary tyrosine-phosphorylated proteins
title_sort early diagnosis of bladder cancer through the detection of urinary tyrosine-phosphorylated proteins
topic Molecular Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522638/
https://www.ncbi.nlm.nih.gov/pubmed/26125446
http://dx.doi.org/10.1038/bjc.2015.232
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