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Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach

Oral squamous cell carcinoma (OSCC) is usually preceded by the oral premalignant lesions, mainly oral leukoplakia (OLK) after repeated insults of carcinogens, tobacco. B(a)P and DMBA are key carcinogens in tobacco smoke. In the present study, for the first time we established the cancerous cell line...

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Autores principales: Dong, Yan, Zhao, Qun, Ma, Xiaoyan, Ma, Guowu, Liu, Caiyun, Chen, Zhuwen, Yu, Liyuan, Liu, Xuefeng, Zhang, Yanguang, Shao, Shujuan, Xiao, Jing, Li, Jia, Zhang, Weimin, Fu, Ming, Dong, Lijia, Yang, Xiandong, Guo, Xu, Xue, Liyan, Fang, Fei, Zhan, Qimin, Zhang, Lihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522661/
https://www.ncbi.nlm.nih.gov/pubmed/26234610
http://dx.doi.org/10.1038/srep12668
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author Dong, Yan
Zhao, Qun
Ma, Xiaoyan
Ma, Guowu
Liu, Caiyun
Chen, Zhuwen
Yu, Liyuan
Liu, Xuefeng
Zhang, Yanguang
Shao, Shujuan
Xiao, Jing
Li, Jia
Zhang, Weimin
Fu, Ming
Dong, Lijia
Yang, Xiandong
Guo, Xu
Xue, Liyan
Fang, Fei
Zhan, Qimin
Zhang, Lihua
author_facet Dong, Yan
Zhao, Qun
Ma, Xiaoyan
Ma, Guowu
Liu, Caiyun
Chen, Zhuwen
Yu, Liyuan
Liu, Xuefeng
Zhang, Yanguang
Shao, Shujuan
Xiao, Jing
Li, Jia
Zhang, Weimin
Fu, Ming
Dong, Lijia
Yang, Xiandong
Guo, Xu
Xue, Liyan
Fang, Fei
Zhan, Qimin
Zhang, Lihua
author_sort Dong, Yan
collection PubMed
description Oral squamous cell carcinoma (OSCC) is usually preceded by the oral premalignant lesions, mainly oral leukoplakia (OLK) after repeated insults of carcinogens, tobacco. B(a)P and DMBA are key carcinogens in tobacco smoke. In the present study, for the first time we established the cancerous cell line OSCC-BD induced by B(a)P/DMBA mixture and transformed from dysplastic oral leukoplakia cell line DOK. Cell morphology, proliferation ability, migration ability, colony formation, and tumorigenicity were studied and confirmed the malignant characteristics of OSCC-BD cells. We further identified the differential proteins between DOK and OSCC-BD cells by stable isotope dimethyl labeling based quantitative proteomic method, which showed 18 proteins up-regulated and 16 proteins down-regulated with RSD < 8%. Differential proteins are mainly related to cell cycle, cell proliferation, DNA replication, RNA splicing and apoptosis. Abberant binding function, catalysis activity and transportor activity of differential proteins might contribute to the malignant transformation of OLK. Of the 34 identified differential proteins with RSD < 8%, 13 novel cancer-related proteins were reported in the present study. This study might provide a new insight into the mechanism of OLK malignant transformation and the potent biomarkers for early diagnosis, meanwhile further facilitate the application of the quantification proteomics to carcinogenesis research.
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spelling pubmed-45226612015-08-06 Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach Dong, Yan Zhao, Qun Ma, Xiaoyan Ma, Guowu Liu, Caiyun Chen, Zhuwen Yu, Liyuan Liu, Xuefeng Zhang, Yanguang Shao, Shujuan Xiao, Jing Li, Jia Zhang, Weimin Fu, Ming Dong, Lijia Yang, Xiandong Guo, Xu Xue, Liyan Fang, Fei Zhan, Qimin Zhang, Lihua Sci Rep Article Oral squamous cell carcinoma (OSCC) is usually preceded by the oral premalignant lesions, mainly oral leukoplakia (OLK) after repeated insults of carcinogens, tobacco. B(a)P and DMBA are key carcinogens in tobacco smoke. In the present study, for the first time we established the cancerous cell line OSCC-BD induced by B(a)P/DMBA mixture and transformed from dysplastic oral leukoplakia cell line DOK. Cell morphology, proliferation ability, migration ability, colony formation, and tumorigenicity were studied and confirmed the malignant characteristics of OSCC-BD cells. We further identified the differential proteins between DOK and OSCC-BD cells by stable isotope dimethyl labeling based quantitative proteomic method, which showed 18 proteins up-regulated and 16 proteins down-regulated with RSD < 8%. Differential proteins are mainly related to cell cycle, cell proliferation, DNA replication, RNA splicing and apoptosis. Abberant binding function, catalysis activity and transportor activity of differential proteins might contribute to the malignant transformation of OLK. Of the 34 identified differential proteins with RSD < 8%, 13 novel cancer-related proteins were reported in the present study. This study might provide a new insight into the mechanism of OLK malignant transformation and the potent biomarkers for early diagnosis, meanwhile further facilitate the application of the quantification proteomics to carcinogenesis research. Nature Publishing Group 2015-08-03 /pmc/articles/PMC4522661/ /pubmed/26234610 http://dx.doi.org/10.1038/srep12668 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Dong, Yan
Zhao, Qun
Ma, Xiaoyan
Ma, Guowu
Liu, Caiyun
Chen, Zhuwen
Yu, Liyuan
Liu, Xuefeng
Zhang, Yanguang
Shao, Shujuan
Xiao, Jing
Li, Jia
Zhang, Weimin
Fu, Ming
Dong, Lijia
Yang, Xiandong
Guo, Xu
Xue, Liyan
Fang, Fei
Zhan, Qimin
Zhang, Lihua
Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach
title Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach
title_full Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach
title_fullStr Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach
title_full_unstemmed Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach
title_short Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach
title_sort establishment of a new oscc cell line derived from olk and identification of malignant transformation-related proteins by differential proteomics approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522661/
https://www.ncbi.nlm.nih.gov/pubmed/26234610
http://dx.doi.org/10.1038/srep12668
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