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Isolation and in silico characterization of novel esterase gene with β-lactamase fold isolated from metagenome of north western Himalayas

An esterase-producing clone Aph2 was isolated from the Apharwat soil metagenomic library, a mountain peak in NW Himalayas. ORF 2 (Est Ac) of clone Aph2 corresponds to 271 aa protein and showed 26 % sequence similarity to carboxylesterase gene of Synechococcus sp. JA-2-3B. Est Ac contains nucleophili...

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Detalles Bibliográficos
Autores principales: Sudan, Avneet Kour, Vakhlu, Jyoti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522730/
https://www.ncbi.nlm.nih.gov/pubmed/28324560
http://dx.doi.org/10.1007/s13205-014-0254-5