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Isolation and in silico characterization of novel esterase gene with β-lactamase fold isolated from metagenome of north western Himalayas
An esterase-producing clone Aph2 was isolated from the Apharwat soil metagenomic library, a mountain peak in NW Himalayas. ORF 2 (Est Ac) of clone Aph2 corresponds to 271 aa protein and showed 26 % sequence similarity to carboxylesterase gene of Synechococcus sp. JA-2-3B. Est Ac contains nucleophili...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Springer Berlin Heidelberg
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522730/ https://www.ncbi.nlm.nih.gov/pubmed/28324560 http://dx.doi.org/10.1007/s13205-014-0254-5 |