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Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana

Diatoms are unicellular algae responsible for approximately 20% of global carbon fixation. Their evolution by secondary endocytobiosis resulted in a complex cellular structure and metabolism compared to algae with primary plastids. The sulfate assimilation and methionine synthesis pathways provide S...

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Autores principales: Bromke, Mariusz A, Hesse, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523565/
https://www.ncbi.nlm.nih.gov/pubmed/26251775
http://dx.doi.org/10.1186/s40064-015-1163-8
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author Bromke, Mariusz A
Hesse, Holger
author_facet Bromke, Mariusz A
Hesse, Holger
author_sort Bromke, Mariusz A
collection PubMed
description Diatoms are unicellular algae responsible for approximately 20% of global carbon fixation. Their evolution by secondary endocytobiosis resulted in a complex cellular structure and metabolism compared to algae with primary plastids. The sulfate assimilation and methionine synthesis pathways provide S-containing amino acids for the synthesis of proteins and a range of metabolites such as dimethylsulfoniopropionate. To obtain an insight into the localization and organization of the sulfur metabolism pathways we surveyed the genome of Thalassiosira pseudonana—a model organism for diatom research. We have identified and annotated genes for enzymes involved in respective pathways. Protein localization was predicted using similarities to known signal peptide motifs. We performed detailed phylogenetic analyses of enzymes involved in sulfate uptake/reduction and methionine metabolism. Moreover, we have found in up-stream sequences of studied diatoms methionine biosynthesis genes a conserved motif, which shows similarity to the Met31, a cis-motif regulating expression of methionine biosynthesis genes in yeast.
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spelling pubmed-45235652015-08-06 Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana Bromke, Mariusz A Hesse, Holger Springerplus Research Diatoms are unicellular algae responsible for approximately 20% of global carbon fixation. Their evolution by secondary endocytobiosis resulted in a complex cellular structure and metabolism compared to algae with primary plastids. The sulfate assimilation and methionine synthesis pathways provide S-containing amino acids for the synthesis of proteins and a range of metabolites such as dimethylsulfoniopropionate. To obtain an insight into the localization and organization of the sulfur metabolism pathways we surveyed the genome of Thalassiosira pseudonana—a model organism for diatom research. We have identified and annotated genes for enzymes involved in respective pathways. Protein localization was predicted using similarities to known signal peptide motifs. We performed detailed phylogenetic analyses of enzymes involved in sulfate uptake/reduction and methionine metabolism. Moreover, we have found in up-stream sequences of studied diatoms methionine biosynthesis genes a conserved motif, which shows similarity to the Met31, a cis-motif regulating expression of methionine biosynthesis genes in yeast. Springer International Publishing 2015-08-04 /pmc/articles/PMC4523565/ /pubmed/26251775 http://dx.doi.org/10.1186/s40064-015-1163-8 Text en © Bromke and Hesse. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Bromke, Mariusz A
Hesse, Holger
Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana
title Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana
title_full Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana
title_fullStr Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana
title_full_unstemmed Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana
title_short Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana
title_sort phylogenetic analysis of methionine synthesis genes from thalassiosira pseudonana
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523565/
https://www.ncbi.nlm.nih.gov/pubmed/26251775
http://dx.doi.org/10.1186/s40064-015-1163-8
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