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Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana
Diatoms are unicellular algae responsible for approximately 20% of global carbon fixation. Their evolution by secondary endocytobiosis resulted in a complex cellular structure and metabolism compared to algae with primary plastids. The sulfate assimilation and methionine synthesis pathways provide S...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523565/ https://www.ncbi.nlm.nih.gov/pubmed/26251775 http://dx.doi.org/10.1186/s40064-015-1163-8 |
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author | Bromke, Mariusz A Hesse, Holger |
author_facet | Bromke, Mariusz A Hesse, Holger |
author_sort | Bromke, Mariusz A |
collection | PubMed |
description | Diatoms are unicellular algae responsible for approximately 20% of global carbon fixation. Their evolution by secondary endocytobiosis resulted in a complex cellular structure and metabolism compared to algae with primary plastids. The sulfate assimilation and methionine synthesis pathways provide S-containing amino acids for the synthesis of proteins and a range of metabolites such as dimethylsulfoniopropionate. To obtain an insight into the localization and organization of the sulfur metabolism pathways we surveyed the genome of Thalassiosira pseudonana—a model organism for diatom research. We have identified and annotated genes for enzymes involved in respective pathways. Protein localization was predicted using similarities to known signal peptide motifs. We performed detailed phylogenetic analyses of enzymes involved in sulfate uptake/reduction and methionine metabolism. Moreover, we have found in up-stream sequences of studied diatoms methionine biosynthesis genes a conserved motif, which shows similarity to the Met31, a cis-motif regulating expression of methionine biosynthesis genes in yeast. |
format | Online Article Text |
id | pubmed-4523565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-45235652015-08-06 Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana Bromke, Mariusz A Hesse, Holger Springerplus Research Diatoms are unicellular algae responsible for approximately 20% of global carbon fixation. Their evolution by secondary endocytobiosis resulted in a complex cellular structure and metabolism compared to algae with primary plastids. The sulfate assimilation and methionine synthesis pathways provide S-containing amino acids for the synthesis of proteins and a range of metabolites such as dimethylsulfoniopropionate. To obtain an insight into the localization and organization of the sulfur metabolism pathways we surveyed the genome of Thalassiosira pseudonana—a model organism for diatom research. We have identified and annotated genes for enzymes involved in respective pathways. Protein localization was predicted using similarities to known signal peptide motifs. We performed detailed phylogenetic analyses of enzymes involved in sulfate uptake/reduction and methionine metabolism. Moreover, we have found in up-stream sequences of studied diatoms methionine biosynthesis genes a conserved motif, which shows similarity to the Met31, a cis-motif regulating expression of methionine biosynthesis genes in yeast. Springer International Publishing 2015-08-04 /pmc/articles/PMC4523565/ /pubmed/26251775 http://dx.doi.org/10.1186/s40064-015-1163-8 Text en © Bromke and Hesse. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Bromke, Mariusz A Hesse, Holger Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana |
title | Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana |
title_full | Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana |
title_fullStr | Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana |
title_full_unstemmed | Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana |
title_short | Phylogenetic analysis of methionine synthesis genes from Thalassiosira pseudonana |
title_sort | phylogenetic analysis of methionine synthesis genes from thalassiosira pseudonana |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523565/ https://www.ncbi.nlm.nih.gov/pubmed/26251775 http://dx.doi.org/10.1186/s40064-015-1163-8 |
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