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Direct conscious telemetry recordings demonstrate increased renal sympathetic nerve activity in rats with chronic kidney disease

Chronic kidney disease (CKD) is associated with sympathetic hyperactivity and impaired blood pressure control reflex responses, yet direct evidence demonstrating these features of autonomic dysfunction in conscious animals is still lacking. Here we measured renal sympathetic nerve activity (RSNA) an...

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Autores principales: Salman, Ibrahim M., Sarma Kandukuri, Divya, Harrison, Joanne L., Hildreth, Cara M., Phillips, Jacqueline K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523722/
https://www.ncbi.nlm.nih.gov/pubmed/26300784
http://dx.doi.org/10.3389/fphys.2015.00218
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author Salman, Ibrahim M.
Sarma Kandukuri, Divya
Harrison, Joanne L.
Hildreth, Cara M.
Phillips, Jacqueline K.
author_facet Salman, Ibrahim M.
Sarma Kandukuri, Divya
Harrison, Joanne L.
Hildreth, Cara M.
Phillips, Jacqueline K.
author_sort Salman, Ibrahim M.
collection PubMed
description Chronic kidney disease (CKD) is associated with sympathetic hyperactivity and impaired blood pressure control reflex responses, yet direct evidence demonstrating these features of autonomic dysfunction in conscious animals is still lacking. Here we measured renal sympathetic nerve activity (RSNA) and mean arterial pressure (MAP) using telemetry-based recordings in a rat model of CKD, the Lewis Polycystic Kidney (LPK) rat, and assessed responses to chemoreflex activation and acute stress. Male LPK and Lewis control animals (total n = 16) were instrumented for telemetric recording of RSNA and MAP. At 12–13 weeks-of-age, resting RSNA and MAP, sympathetic and haemodynamic responses to both peripheral (hypoxia: 10% O(2)) and central chemoreflex (hypercapnia: 7% CO(2)) activation and acute stress (open-field exposure), were measured. As indicators of renal function, urinary protein (U(Pro)) and creatinine (U(Cr)) levels were assessed. LPK rats had higher resting RSNA (1.2 ± 0.1 vs. 0.6 ± 0.1 μV, p < 0.05) and MAP (151 ± 8 vs. 97 ± 2 mmHg, p < 0.05) compared to Lewis. MAP was negatively correlated with U(Cr) (r = −0.80, p = 0.002) and positively correlated with RSNA (r = 0.66, p = 0.014), with multiple linear regression modeling indicating the strongest correlation was with U(cr). RSNA and MAP responses to activation of the central chemoreflex and open-field stress were reduced in the LPK relative to the Lewis (all p < 0.05). This is the first description of dual conscious telemetry recording of RSNA and MAP in a genetic rodent model of CKD. Elevated RSNA is likely a key contributor to the marked hypertension in this model, while attenuated RSNA and MAP responses to central chemoreflex activation and acute stress in the LPK indicate possible deficits in the neural processing of autonomic outflows evoked by these sympathoexcitatory pathways.
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spelling pubmed-45237222015-08-21 Direct conscious telemetry recordings demonstrate increased renal sympathetic nerve activity in rats with chronic kidney disease Salman, Ibrahim M. Sarma Kandukuri, Divya Harrison, Joanne L. Hildreth, Cara M. Phillips, Jacqueline K. Front Physiol Physiology Chronic kidney disease (CKD) is associated with sympathetic hyperactivity and impaired blood pressure control reflex responses, yet direct evidence demonstrating these features of autonomic dysfunction in conscious animals is still lacking. Here we measured renal sympathetic nerve activity (RSNA) and mean arterial pressure (MAP) using telemetry-based recordings in a rat model of CKD, the Lewis Polycystic Kidney (LPK) rat, and assessed responses to chemoreflex activation and acute stress. Male LPK and Lewis control animals (total n = 16) were instrumented for telemetric recording of RSNA and MAP. At 12–13 weeks-of-age, resting RSNA and MAP, sympathetic and haemodynamic responses to both peripheral (hypoxia: 10% O(2)) and central chemoreflex (hypercapnia: 7% CO(2)) activation and acute stress (open-field exposure), were measured. As indicators of renal function, urinary protein (U(Pro)) and creatinine (U(Cr)) levels were assessed. LPK rats had higher resting RSNA (1.2 ± 0.1 vs. 0.6 ± 0.1 μV, p < 0.05) and MAP (151 ± 8 vs. 97 ± 2 mmHg, p < 0.05) compared to Lewis. MAP was negatively correlated with U(Cr) (r = −0.80, p = 0.002) and positively correlated with RSNA (r = 0.66, p = 0.014), with multiple linear regression modeling indicating the strongest correlation was with U(cr). RSNA and MAP responses to activation of the central chemoreflex and open-field stress were reduced in the LPK relative to the Lewis (all p < 0.05). This is the first description of dual conscious telemetry recording of RSNA and MAP in a genetic rodent model of CKD. Elevated RSNA is likely a key contributor to the marked hypertension in this model, while attenuated RSNA and MAP responses to central chemoreflex activation and acute stress in the LPK indicate possible deficits in the neural processing of autonomic outflows evoked by these sympathoexcitatory pathways. Frontiers Media S.A. 2015-08-04 /pmc/articles/PMC4523722/ /pubmed/26300784 http://dx.doi.org/10.3389/fphys.2015.00218 Text en Copyright © 2015 Salman, Sarma Kandukuri, Harrison, Hildreth and Phillips. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Salman, Ibrahim M.
Sarma Kandukuri, Divya
Harrison, Joanne L.
Hildreth, Cara M.
Phillips, Jacqueline K.
Direct conscious telemetry recordings demonstrate increased renal sympathetic nerve activity in rats with chronic kidney disease
title Direct conscious telemetry recordings demonstrate increased renal sympathetic nerve activity in rats with chronic kidney disease
title_full Direct conscious telemetry recordings demonstrate increased renal sympathetic nerve activity in rats with chronic kidney disease
title_fullStr Direct conscious telemetry recordings demonstrate increased renal sympathetic nerve activity in rats with chronic kidney disease
title_full_unstemmed Direct conscious telemetry recordings demonstrate increased renal sympathetic nerve activity in rats with chronic kidney disease
title_short Direct conscious telemetry recordings demonstrate increased renal sympathetic nerve activity in rats with chronic kidney disease
title_sort direct conscious telemetry recordings demonstrate increased renal sympathetic nerve activity in rats with chronic kidney disease
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523722/
https://www.ncbi.nlm.nih.gov/pubmed/26300784
http://dx.doi.org/10.3389/fphys.2015.00218
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