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Leptin, its receptor and aromatase expression in deep infiltrating endometriosis

BACKGROUND: The aim of this study was to evaluate the leptin levels in the serum and peritoneal fluid (PF) and the protein expression in three different peritoneal ectopic implants in patients who underwent surgery for deep infiltrating endometriosis. METHODS: All patients had been treated at the De...

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Autores principales: Gonçalves, Helder F., Zendron, Carolina, Cavalcante, Fernanda S., Aiceles, Verônica, Oliveira, Marco Aurélio P., Manaia, Jorge Henrique M., Babinski, Márcio A., Ramos, Cristiane F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523920/
https://www.ncbi.nlm.nih.gov/pubmed/26242176
http://dx.doi.org/10.1186/s13048-015-0180-0
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author Gonçalves, Helder F.
Zendron, Carolina
Cavalcante, Fernanda S.
Aiceles, Verônica
Oliveira, Marco Aurélio P.
Manaia, Jorge Henrique M.
Babinski, Márcio A.
Ramos, Cristiane F.
author_facet Gonçalves, Helder F.
Zendron, Carolina
Cavalcante, Fernanda S.
Aiceles, Verônica
Oliveira, Marco Aurélio P.
Manaia, Jorge Henrique M.
Babinski, Márcio A.
Ramos, Cristiane F.
author_sort Gonçalves, Helder F.
collection PubMed
description BACKGROUND: The aim of this study was to evaluate the leptin levels in the serum and peritoneal fluid (PF) and the protein expression in three different peritoneal ectopic implants in patients who underwent surgery for deep infiltrating endometriosis. METHODS: All patients had been treated at the Department of Gynecology of the Pedro Ernesto University Hospital, Rio de Janeiro. The study group consisted of 15 patients who underwent surgery for adnexal masses and infertility, while the control group consisted of ten women who underwent surgery for tubal ligation. Peritoneal fluid and samples tissues were collected during surgery. Serum samples were obtained before anesthesia. In this study, the leptin levels in the serum and peritoneal fluid (PF) were evaluated by ELISA. The protein expression of leptin and its receptors (ObR) and aromatase enzyme were evaluated by Western blot analysis of the intestine, uterosacral ligament and vaginal septum in the ectopic implants. The t-test and one-way ANOVA with Holm-Sìdak post-test were used, and p < 0.05 was considered to be statistically significant. RESULTS: Compared to the controls, the serum leptin levels (control = 14.7 ng/mL ± 2.63, endometriosis = 19.2 ng/mL ± 1.84, p < 0.0001) were increased, while in PF, there was no difference (control = 6.68 ng/mL ± 0.43, endometriosis = 7.71 ng/mL ± 0.59, p = 0.18). Comparing women with and without ovarian implants, the leptin levels in both the serum and PF were significantly higher in women without ovarian implants (serum: with ovarian implant = 15.85 ± 1.99; without ovarian implant = 23.14 ± 2.60; ng/mL, p = 0.04; PF: with ovarian implant = 4.28 ± 1.30; without ovarian implant = 11.18 ± 2.98;ng/mL, p = 0.048). The leptin, ObR and aromatase protein expression levels were increased in lesions in the vaginal septum and were decreased in the intestine lesions. CONCLUSION: This study reports several interesting associations between the leptin levels in serum, peritoneal fluid, and tissue samples and the localization of the ectopic endometrium. Although this study does not provide a clear picture of the role of leptin in the development and progression of peritoneal implants, it contributed new data that might be useful to elucidating the enigma that is the role of leptin in endometriosis disease.
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spelling pubmed-45239202015-08-05 Leptin, its receptor and aromatase expression in deep infiltrating endometriosis Gonçalves, Helder F. Zendron, Carolina Cavalcante, Fernanda S. Aiceles, Verônica Oliveira, Marco Aurélio P. Manaia, Jorge Henrique M. Babinski, Márcio A. Ramos, Cristiane F. J Ovarian Res Research BACKGROUND: The aim of this study was to evaluate the leptin levels in the serum and peritoneal fluid (PF) and the protein expression in three different peritoneal ectopic implants in patients who underwent surgery for deep infiltrating endometriosis. METHODS: All patients had been treated at the Department of Gynecology of the Pedro Ernesto University Hospital, Rio de Janeiro. The study group consisted of 15 patients who underwent surgery for adnexal masses and infertility, while the control group consisted of ten women who underwent surgery for tubal ligation. Peritoneal fluid and samples tissues were collected during surgery. Serum samples were obtained before anesthesia. In this study, the leptin levels in the serum and peritoneal fluid (PF) were evaluated by ELISA. The protein expression of leptin and its receptors (ObR) and aromatase enzyme were evaluated by Western blot analysis of the intestine, uterosacral ligament and vaginal septum in the ectopic implants. The t-test and one-way ANOVA with Holm-Sìdak post-test were used, and p < 0.05 was considered to be statistically significant. RESULTS: Compared to the controls, the serum leptin levels (control = 14.7 ng/mL ± 2.63, endometriosis = 19.2 ng/mL ± 1.84, p < 0.0001) were increased, while in PF, there was no difference (control = 6.68 ng/mL ± 0.43, endometriosis = 7.71 ng/mL ± 0.59, p = 0.18). Comparing women with and without ovarian implants, the leptin levels in both the serum and PF were significantly higher in women without ovarian implants (serum: with ovarian implant = 15.85 ± 1.99; without ovarian implant = 23.14 ± 2.60; ng/mL, p = 0.04; PF: with ovarian implant = 4.28 ± 1.30; without ovarian implant = 11.18 ± 2.98;ng/mL, p = 0.048). The leptin, ObR and aromatase protein expression levels were increased in lesions in the vaginal septum and were decreased in the intestine lesions. CONCLUSION: This study reports several interesting associations between the leptin levels in serum, peritoneal fluid, and tissue samples and the localization of the ectopic endometrium. Although this study does not provide a clear picture of the role of leptin in the development and progression of peritoneal implants, it contributed new data that might be useful to elucidating the enigma that is the role of leptin in endometriosis disease. BioMed Central 2015-08-05 /pmc/articles/PMC4523920/ /pubmed/26242176 http://dx.doi.org/10.1186/s13048-015-0180-0 Text en © Gonçalves et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Gonçalves, Helder F.
Zendron, Carolina
Cavalcante, Fernanda S.
Aiceles, Verônica
Oliveira, Marco Aurélio P.
Manaia, Jorge Henrique M.
Babinski, Márcio A.
Ramos, Cristiane F.
Leptin, its receptor and aromatase expression in deep infiltrating endometriosis
title Leptin, its receptor and aromatase expression in deep infiltrating endometriosis
title_full Leptin, its receptor and aromatase expression in deep infiltrating endometriosis
title_fullStr Leptin, its receptor and aromatase expression in deep infiltrating endometriosis
title_full_unstemmed Leptin, its receptor and aromatase expression in deep infiltrating endometriosis
title_short Leptin, its receptor and aromatase expression in deep infiltrating endometriosis
title_sort leptin, its receptor and aromatase expression in deep infiltrating endometriosis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523920/
https://www.ncbi.nlm.nih.gov/pubmed/26242176
http://dx.doi.org/10.1186/s13048-015-0180-0
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