Cargando…
Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast
The article demonstrates that computational modeling has the capacity to convert metabolic snapshots, taken sequentially over time, into a description of cellular, dynamic strategies. The specific application is a detailed analysis of a set of actions with which Saccharomyces cerevisiae responds to...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524633/ https://www.ncbi.nlm.nih.gov/pubmed/26241868 http://dx.doi.org/10.1371/journal.pcbi.1004373 |
_version_ | 1782384223076745216 |
---|---|
author | Chen, Po-Wei Fonseca, Luis L. Hannun, Yusuf A. Voit, Eberhard O. |
author_facet | Chen, Po-Wei Fonseca, Luis L. Hannun, Yusuf A. Voit, Eberhard O. |
author_sort | Chen, Po-Wei |
collection | PubMed |
description | The article demonstrates that computational modeling has the capacity to convert metabolic snapshots, taken sequentially over time, into a description of cellular, dynamic strategies. The specific application is a detailed analysis of a set of actions with which Saccharomyces cerevisiae responds to heat stress. Using time dependent metabolic concentration data, we use a combination of mathematical modeling, reverse engineering, and optimization to infer dynamic changes in enzyme activities within the sphingolipid pathway. The details of the sphingolipid responses to heat stress are important, because they guide some of the longer-term alterations in gene expression, with which the cells adapt to the increased temperature. The analysis indicates that all enzyme activities in the system are affected and that the shapes of the time trends in activities depend on the fatty-acyl CoA chain lengths of the different ceramide species in the system. |
format | Online Article Text |
id | pubmed-4524633 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45246332015-08-06 Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast Chen, Po-Wei Fonseca, Luis L. Hannun, Yusuf A. Voit, Eberhard O. PLoS Comput Biol Research Article The article demonstrates that computational modeling has the capacity to convert metabolic snapshots, taken sequentially over time, into a description of cellular, dynamic strategies. The specific application is a detailed analysis of a set of actions with which Saccharomyces cerevisiae responds to heat stress. Using time dependent metabolic concentration data, we use a combination of mathematical modeling, reverse engineering, and optimization to infer dynamic changes in enzyme activities within the sphingolipid pathway. The details of the sphingolipid responses to heat stress are important, because they guide some of the longer-term alterations in gene expression, with which the cells adapt to the increased temperature. The analysis indicates that all enzyme activities in the system are affected and that the shapes of the time trends in activities depend on the fatty-acyl CoA chain lengths of the different ceramide species in the system. Public Library of Science 2015-08-04 /pmc/articles/PMC4524633/ /pubmed/26241868 http://dx.doi.org/10.1371/journal.pcbi.1004373 Text en © 2015 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chen, Po-Wei Fonseca, Luis L. Hannun, Yusuf A. Voit, Eberhard O. Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast |
title | Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast |
title_full | Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast |
title_fullStr | Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast |
title_full_unstemmed | Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast |
title_short | Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast |
title_sort | dynamics of the heat stress response of ceramides with different fatty-acyl chain lengths in baker’s yeast |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524633/ https://www.ncbi.nlm.nih.gov/pubmed/26241868 http://dx.doi.org/10.1371/journal.pcbi.1004373 |
work_keys_str_mv | AT chenpowei dynamicsoftheheatstressresponseofceramideswithdifferentfattyacylchainlengthsinbakersyeast AT fonsecaluisl dynamicsoftheheatstressresponseofceramideswithdifferentfattyacylchainlengthsinbakersyeast AT hannunyusufa dynamicsoftheheatstressresponseofceramideswithdifferentfattyacylchainlengthsinbakersyeast AT voiteberhardo dynamicsoftheheatstressresponseofceramideswithdifferentfattyacylchainlengthsinbakersyeast |