Cargando…

Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast

The article demonstrates that computational modeling has the capacity to convert metabolic snapshots, taken sequentially over time, into a description of cellular, dynamic strategies. The specific application is a detailed analysis of a set of actions with which Saccharomyces cerevisiae responds to...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Po-Wei, Fonseca, Luis L., Hannun, Yusuf A., Voit, Eberhard O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524633/
https://www.ncbi.nlm.nih.gov/pubmed/26241868
http://dx.doi.org/10.1371/journal.pcbi.1004373
_version_ 1782384223076745216
author Chen, Po-Wei
Fonseca, Luis L.
Hannun, Yusuf A.
Voit, Eberhard O.
author_facet Chen, Po-Wei
Fonseca, Luis L.
Hannun, Yusuf A.
Voit, Eberhard O.
author_sort Chen, Po-Wei
collection PubMed
description The article demonstrates that computational modeling has the capacity to convert metabolic snapshots, taken sequentially over time, into a description of cellular, dynamic strategies. The specific application is a detailed analysis of a set of actions with which Saccharomyces cerevisiae responds to heat stress. Using time dependent metabolic concentration data, we use a combination of mathematical modeling, reverse engineering, and optimization to infer dynamic changes in enzyme activities within the sphingolipid pathway. The details of the sphingolipid responses to heat stress are important, because they guide some of the longer-term alterations in gene expression, with which the cells adapt to the increased temperature. The analysis indicates that all enzyme activities in the system are affected and that the shapes of the time trends in activities depend on the fatty-acyl CoA chain lengths of the different ceramide species in the system.
format Online
Article
Text
id pubmed-4524633
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-45246332015-08-06 Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast Chen, Po-Wei Fonseca, Luis L. Hannun, Yusuf A. Voit, Eberhard O. PLoS Comput Biol Research Article The article demonstrates that computational modeling has the capacity to convert metabolic snapshots, taken sequentially over time, into a description of cellular, dynamic strategies. The specific application is a detailed analysis of a set of actions with which Saccharomyces cerevisiae responds to heat stress. Using time dependent metabolic concentration data, we use a combination of mathematical modeling, reverse engineering, and optimization to infer dynamic changes in enzyme activities within the sphingolipid pathway. The details of the sphingolipid responses to heat stress are important, because they guide some of the longer-term alterations in gene expression, with which the cells adapt to the increased temperature. The analysis indicates that all enzyme activities in the system are affected and that the shapes of the time trends in activities depend on the fatty-acyl CoA chain lengths of the different ceramide species in the system. Public Library of Science 2015-08-04 /pmc/articles/PMC4524633/ /pubmed/26241868 http://dx.doi.org/10.1371/journal.pcbi.1004373 Text en © 2015 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Po-Wei
Fonseca, Luis L.
Hannun, Yusuf A.
Voit, Eberhard O.
Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast
title Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast
title_full Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast
title_fullStr Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast
title_full_unstemmed Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast
title_short Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl Chain Lengths in Baker’s Yeast
title_sort dynamics of the heat stress response of ceramides with different fatty-acyl chain lengths in baker’s yeast
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524633/
https://www.ncbi.nlm.nih.gov/pubmed/26241868
http://dx.doi.org/10.1371/journal.pcbi.1004373
work_keys_str_mv AT chenpowei dynamicsoftheheatstressresponseofceramideswithdifferentfattyacylchainlengthsinbakersyeast
AT fonsecaluisl dynamicsoftheheatstressresponseofceramideswithdifferentfattyacylchainlengthsinbakersyeast
AT hannunyusufa dynamicsoftheheatstressresponseofceramideswithdifferentfattyacylchainlengthsinbakersyeast
AT voiteberhardo dynamicsoftheheatstressresponseofceramideswithdifferentfattyacylchainlengthsinbakersyeast