Cargando…

Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content

Sugarcane is an important crop worldwide for sugar and first generation ethanol production. Recently, the residue of sugarcane mills, named bagasse, has been considered a promising lignocellulosic biomass to produce the second-generation ethanol. Lignin is a major factor limiting the use of bagasse...

Descripción completa

Detalles Bibliográficos
Autores principales: Vicentini, Renato, Bottcher, Alexandra, Brito, Michael dos Santos, dos Santos, Adriana Brombini, Creste, Silvana, Landell, Marcos Guimarães de Andrade, Cesarino, Igor, Mazzafera, Paulo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524650/
https://www.ncbi.nlm.nih.gov/pubmed/26241317
http://dx.doi.org/10.1371/journal.pone.0134909
_version_ 1782384226038972416
author Vicentini, Renato
Bottcher, Alexandra
Brito, Michael dos Santos
dos Santos, Adriana Brombini
Creste, Silvana
Landell, Marcos Guimarães de Andrade
Cesarino, Igor
Mazzafera, Paulo
author_facet Vicentini, Renato
Bottcher, Alexandra
Brito, Michael dos Santos
dos Santos, Adriana Brombini
Creste, Silvana
Landell, Marcos Guimarães de Andrade
Cesarino, Igor
Mazzafera, Paulo
author_sort Vicentini, Renato
collection PubMed
description Sugarcane is an important crop worldwide for sugar and first generation ethanol production. Recently, the residue of sugarcane mills, named bagasse, has been considered a promising lignocellulosic biomass to produce the second-generation ethanol. Lignin is a major factor limiting the use of bagasse and other plant lignocellulosic materials to produce second-generation ethanol. Lignin biosynthesis pathway is a complex network and changes in the expression of genes of this pathway have in general led to diverse and undesirable impacts on plant structure and physiology. Despite its economic importance, sugarcane genome was still not sequenced. In this study a high-throughput transcriptome evaluation of two sugarcane genotypes contrasting for lignin content was carried out. We generated a set of 85,151 transcripts of sugarcane using RNA-seq and de novo assembling. More than 2,000 transcripts showed differential expression between the genotypes, including several genes involved in the lignin biosynthetic pathway. This information can give valuable knowledge on the lignin biosynthesis and its interactions with other metabolic pathways in the complex sugarcane genome.
format Online
Article
Text
id pubmed-4524650
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-45246502015-08-06 Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content Vicentini, Renato Bottcher, Alexandra Brito, Michael dos Santos dos Santos, Adriana Brombini Creste, Silvana Landell, Marcos Guimarães de Andrade Cesarino, Igor Mazzafera, Paulo PLoS One Research Article Sugarcane is an important crop worldwide for sugar and first generation ethanol production. Recently, the residue of sugarcane mills, named bagasse, has been considered a promising lignocellulosic biomass to produce the second-generation ethanol. Lignin is a major factor limiting the use of bagasse and other plant lignocellulosic materials to produce second-generation ethanol. Lignin biosynthesis pathway is a complex network and changes in the expression of genes of this pathway have in general led to diverse and undesirable impacts on plant structure and physiology. Despite its economic importance, sugarcane genome was still not sequenced. In this study a high-throughput transcriptome evaluation of two sugarcane genotypes contrasting for lignin content was carried out. We generated a set of 85,151 transcripts of sugarcane using RNA-seq and de novo assembling. More than 2,000 transcripts showed differential expression between the genotypes, including several genes involved in the lignin biosynthetic pathway. This information can give valuable knowledge on the lignin biosynthesis and its interactions with other metabolic pathways in the complex sugarcane genome. Public Library of Science 2015-08-04 /pmc/articles/PMC4524650/ /pubmed/26241317 http://dx.doi.org/10.1371/journal.pone.0134909 Text en © 2015 Vicentini et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Vicentini, Renato
Bottcher, Alexandra
Brito, Michael dos Santos
dos Santos, Adriana Brombini
Creste, Silvana
Landell, Marcos Guimarães de Andrade
Cesarino, Igor
Mazzafera, Paulo
Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content
title Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content
title_full Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content
title_fullStr Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content
title_full_unstemmed Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content
title_short Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content
title_sort large-scale transcriptome analysis of two sugarcane genotypes contrasting for lignin content
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524650/
https://www.ncbi.nlm.nih.gov/pubmed/26241317
http://dx.doi.org/10.1371/journal.pone.0134909
work_keys_str_mv AT vicentinirenato largescaletranscriptomeanalysisoftwosugarcanegenotypescontrastingforlignincontent
AT bottcheralexandra largescaletranscriptomeanalysisoftwosugarcanegenotypescontrastingforlignincontent
AT britomichaeldossantos largescaletranscriptomeanalysisoftwosugarcanegenotypescontrastingforlignincontent
AT dossantosadrianabrombini largescaletranscriptomeanalysisoftwosugarcanegenotypescontrastingforlignincontent
AT crestesilvana largescaletranscriptomeanalysisoftwosugarcanegenotypescontrastingforlignincontent
AT landellmarcosguimaraesdeandrade largescaletranscriptomeanalysisoftwosugarcanegenotypescontrastingforlignincontent
AT cesarinoigor largescaletranscriptomeanalysisoftwosugarcanegenotypescontrastingforlignincontent
AT mazzaferapaulo largescaletranscriptomeanalysisoftwosugarcanegenotypescontrastingforlignincontent