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Deficiency of Nuclear Receptor Nur77 Aggravates Mouse Experimental Colitis by Increased NFκB Activity in Macrophages

Nuclear receptor Nur77, also referred to as NR4A1 or TR3, plays an important role in innate and adaptive immunity. Nur77 is crucial in regulating the T helper 1/regulatory T-cell balance, is expressed in macrophages and drives M2 macrophage polarization. In this study we aimed to define the function...

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Autores principales: Hamers, Anouk A. J., van Dam, Laura, Teixeira Duarte, José M., Vos, Mariska, Marinković, Goran, van Tiel, Claudia M., Meijer, Sybren L., van Stalborch, Anne-Marieke, Huveneers, Stephan, te Velde, Anje A., de Jonge, Wouter J., de Vries, Carlie J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524678/
https://www.ncbi.nlm.nih.gov/pubmed/26241646
http://dx.doi.org/10.1371/journal.pone.0133598
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author Hamers, Anouk A. J.
van Dam, Laura
Teixeira Duarte, José M.
Vos, Mariska
Marinković, Goran
van Tiel, Claudia M.
Meijer, Sybren L.
van Stalborch, Anne-Marieke
Huveneers, Stephan
te Velde, Anje A.
de Jonge, Wouter J.
de Vries, Carlie J. M.
author_facet Hamers, Anouk A. J.
van Dam, Laura
Teixeira Duarte, José M.
Vos, Mariska
Marinković, Goran
van Tiel, Claudia M.
Meijer, Sybren L.
van Stalborch, Anne-Marieke
Huveneers, Stephan
te Velde, Anje A.
de Jonge, Wouter J.
de Vries, Carlie J. M.
author_sort Hamers, Anouk A. J.
collection PubMed
description Nuclear receptor Nur77, also referred to as NR4A1 or TR3, plays an important role in innate and adaptive immunity. Nur77 is crucial in regulating the T helper 1/regulatory T-cell balance, is expressed in macrophages and drives M2 macrophage polarization. In this study we aimed to define the function of Nur77 in inflammatory bowel disease. In wild-type and Nur77(-/-) mice, colitis development was studied in dextran sodium sulphate (DSS)- and 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced models. To understand the underlying mechanism, Nur77 was overexpressed in macrophages and gut epithelial cells. Nur77 protein is expressed in colon tissues from Crohn’s disease and Ulcerative colitis patients and colons from colitic mice in inflammatory cells and epithelium. In both mouse colitis models inflammation was increased in Nur77(-/-) mice. A higher neutrophil influx and enhanced IL-6, MCP-1 and KC production was observed in Nur77-deficient colons after DSS-treatment. TNBS-induced influx of T-cells and inflammatory monocytes into the colon was higher in Nur77(-/-) mice, along with increased expression of MCP-1, TNFα and IL-6, and decreased Foxp3 RNA expression, compared to wild-type mice. Overexpression of Nur77 in lipopolysaccharide activated RAW macrophages resulted in up-regulated IL-10 and downregulated TNFα, MIF-1 and MCP-1 mRNA expression through NFκB repression. Nur77 also strongly decreased expression of MCP-1, CXCL1, IL-8, MIP-1α and TNFα in gut epithelial Caco-2 cells. Nur77 overexpression suppresses the inflammatory status of both macrophages and gut epithelial cells and together with the in vivo mouse data this supports that Nur77 has a protective function in experimental colitis. These findings may have implications for development of novel targeted treatment strategies regarding inflammatory bowel disease and other inflammatory diseases.
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spelling pubmed-45246782015-08-06 Deficiency of Nuclear Receptor Nur77 Aggravates Mouse Experimental Colitis by Increased NFκB Activity in Macrophages Hamers, Anouk A. J. van Dam, Laura Teixeira Duarte, José M. Vos, Mariska Marinković, Goran van Tiel, Claudia M. Meijer, Sybren L. van Stalborch, Anne-Marieke Huveneers, Stephan te Velde, Anje A. de Jonge, Wouter J. de Vries, Carlie J. M. PLoS One Research Article Nuclear receptor Nur77, also referred to as NR4A1 or TR3, plays an important role in innate and adaptive immunity. Nur77 is crucial in regulating the T helper 1/regulatory T-cell balance, is expressed in macrophages and drives M2 macrophage polarization. In this study we aimed to define the function of Nur77 in inflammatory bowel disease. In wild-type and Nur77(-/-) mice, colitis development was studied in dextran sodium sulphate (DSS)- and 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced models. To understand the underlying mechanism, Nur77 was overexpressed in macrophages and gut epithelial cells. Nur77 protein is expressed in colon tissues from Crohn’s disease and Ulcerative colitis patients and colons from colitic mice in inflammatory cells and epithelium. In both mouse colitis models inflammation was increased in Nur77(-/-) mice. A higher neutrophil influx and enhanced IL-6, MCP-1 and KC production was observed in Nur77-deficient colons after DSS-treatment. TNBS-induced influx of T-cells and inflammatory monocytes into the colon was higher in Nur77(-/-) mice, along with increased expression of MCP-1, TNFα and IL-6, and decreased Foxp3 RNA expression, compared to wild-type mice. Overexpression of Nur77 in lipopolysaccharide activated RAW macrophages resulted in up-regulated IL-10 and downregulated TNFα, MIF-1 and MCP-1 mRNA expression through NFκB repression. Nur77 also strongly decreased expression of MCP-1, CXCL1, IL-8, MIP-1α and TNFα in gut epithelial Caco-2 cells. Nur77 overexpression suppresses the inflammatory status of both macrophages and gut epithelial cells and together with the in vivo mouse data this supports that Nur77 has a protective function in experimental colitis. These findings may have implications for development of novel targeted treatment strategies regarding inflammatory bowel disease and other inflammatory diseases. Public Library of Science 2015-08-04 /pmc/articles/PMC4524678/ /pubmed/26241646 http://dx.doi.org/10.1371/journal.pone.0133598 Text en © 2015 Hamers et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hamers, Anouk A. J.
van Dam, Laura
Teixeira Duarte, José M.
Vos, Mariska
Marinković, Goran
van Tiel, Claudia M.
Meijer, Sybren L.
van Stalborch, Anne-Marieke
Huveneers, Stephan
te Velde, Anje A.
de Jonge, Wouter J.
de Vries, Carlie J. M.
Deficiency of Nuclear Receptor Nur77 Aggravates Mouse Experimental Colitis by Increased NFκB Activity in Macrophages
title Deficiency of Nuclear Receptor Nur77 Aggravates Mouse Experimental Colitis by Increased NFκB Activity in Macrophages
title_full Deficiency of Nuclear Receptor Nur77 Aggravates Mouse Experimental Colitis by Increased NFκB Activity in Macrophages
title_fullStr Deficiency of Nuclear Receptor Nur77 Aggravates Mouse Experimental Colitis by Increased NFκB Activity in Macrophages
title_full_unstemmed Deficiency of Nuclear Receptor Nur77 Aggravates Mouse Experimental Colitis by Increased NFκB Activity in Macrophages
title_short Deficiency of Nuclear Receptor Nur77 Aggravates Mouse Experimental Colitis by Increased NFκB Activity in Macrophages
title_sort deficiency of nuclear receptor nur77 aggravates mouse experimental colitis by increased nfκb activity in macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524678/
https://www.ncbi.nlm.nih.gov/pubmed/26241646
http://dx.doi.org/10.1371/journal.pone.0133598
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