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Differential Migration of Mesenchymal Stem Cells to Ischemic Regions after Middle Cerebral Artery Occlusion in Rats

To evaluate the optimal timing of mesenchymal stem cell (MSC) transplantation following stroke, rats were transplanted with MSCs at 1 (D1), 4 (D4), and 7 days (D7) after middle cerebral artery occlusion (MCAo). Rats in the D1 group showed a better functional recovery than those in the D4 or D7 group...

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Autores principales: Lee, Soo Hyun, Jin, Kyung Sil, Bang, Oh Young, Kim, Byoung Joon, Park, Soo Jin, Lee, Na Hee, Yoo, Keon Hee, Koo, Hong Hoe, Sung, Ki Woong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524688/
https://www.ncbi.nlm.nih.gov/pubmed/26241653
http://dx.doi.org/10.1371/journal.pone.0134920
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author Lee, Soo Hyun
Jin, Kyung Sil
Bang, Oh Young
Kim, Byoung Joon
Park, Soo Jin
Lee, Na Hee
Yoo, Keon Hee
Koo, Hong Hoe
Sung, Ki Woong
author_facet Lee, Soo Hyun
Jin, Kyung Sil
Bang, Oh Young
Kim, Byoung Joon
Park, Soo Jin
Lee, Na Hee
Yoo, Keon Hee
Koo, Hong Hoe
Sung, Ki Woong
author_sort Lee, Soo Hyun
collection PubMed
description To evaluate the optimal timing of mesenchymal stem cell (MSC) transplantation following stroke, rats were transplanted with MSCs at 1 (D1), 4 (D4), and 7 days (D7) after middle cerebral artery occlusion (MCAo). Rats in the D1 group showed a better functional recovery than those in the D4 or D7 groups after MCAo. MSCs preferentially migrated to the cortex in the D1 group, while the MSCs in the D4 or D7 groups preferentially migrated to the striatum. Interestingly, the level of monocyte chemotactic protein-1 (MCP-1) in the cortex was highest at 1 day after MCAo, while the level of stromal cell-derived factor-1 (SDF-1) in the striatum was lowest at 1 day after MCAo and then increased over time. The pattern of MCP-1 and SDF-1 level changes according to the time after MCAo was consistent with in vivo and in vitro migration patterns of MSCs. The results suggest that an earlier MSC transplantation is associated with a better functional recovery after stroke, which could be explained by the preferential migration of MSCs to the cortex in the early transplantation group. The time-dependent differential expression of MCP-1 and SDF-1 between ischemic regions seemed to mediate the differential migration of MSCs. Highest level of MCP-1 at one day of stroke may induce preferential migration of MSCs to the cortex, then better functional improvement.
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spelling pubmed-45246882015-08-06 Differential Migration of Mesenchymal Stem Cells to Ischemic Regions after Middle Cerebral Artery Occlusion in Rats Lee, Soo Hyun Jin, Kyung Sil Bang, Oh Young Kim, Byoung Joon Park, Soo Jin Lee, Na Hee Yoo, Keon Hee Koo, Hong Hoe Sung, Ki Woong PLoS One Research Article To evaluate the optimal timing of mesenchymal stem cell (MSC) transplantation following stroke, rats were transplanted with MSCs at 1 (D1), 4 (D4), and 7 days (D7) after middle cerebral artery occlusion (MCAo). Rats in the D1 group showed a better functional recovery than those in the D4 or D7 groups after MCAo. MSCs preferentially migrated to the cortex in the D1 group, while the MSCs in the D4 or D7 groups preferentially migrated to the striatum. Interestingly, the level of monocyte chemotactic protein-1 (MCP-1) in the cortex was highest at 1 day after MCAo, while the level of stromal cell-derived factor-1 (SDF-1) in the striatum was lowest at 1 day after MCAo and then increased over time. The pattern of MCP-1 and SDF-1 level changes according to the time after MCAo was consistent with in vivo and in vitro migration patterns of MSCs. The results suggest that an earlier MSC transplantation is associated with a better functional recovery after stroke, which could be explained by the preferential migration of MSCs to the cortex in the early transplantation group. The time-dependent differential expression of MCP-1 and SDF-1 between ischemic regions seemed to mediate the differential migration of MSCs. Highest level of MCP-1 at one day of stroke may induce preferential migration of MSCs to the cortex, then better functional improvement. Public Library of Science 2015-08-04 /pmc/articles/PMC4524688/ /pubmed/26241653 http://dx.doi.org/10.1371/journal.pone.0134920 Text en © 2015 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lee, Soo Hyun
Jin, Kyung Sil
Bang, Oh Young
Kim, Byoung Joon
Park, Soo Jin
Lee, Na Hee
Yoo, Keon Hee
Koo, Hong Hoe
Sung, Ki Woong
Differential Migration of Mesenchymal Stem Cells to Ischemic Regions after Middle Cerebral Artery Occlusion in Rats
title Differential Migration of Mesenchymal Stem Cells to Ischemic Regions after Middle Cerebral Artery Occlusion in Rats
title_full Differential Migration of Mesenchymal Stem Cells to Ischemic Regions after Middle Cerebral Artery Occlusion in Rats
title_fullStr Differential Migration of Mesenchymal Stem Cells to Ischemic Regions after Middle Cerebral Artery Occlusion in Rats
title_full_unstemmed Differential Migration of Mesenchymal Stem Cells to Ischemic Regions after Middle Cerebral Artery Occlusion in Rats
title_short Differential Migration of Mesenchymal Stem Cells to Ischemic Regions after Middle Cerebral Artery Occlusion in Rats
title_sort differential migration of mesenchymal stem cells to ischemic regions after middle cerebral artery occlusion in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524688/
https://www.ncbi.nlm.nih.gov/pubmed/26241653
http://dx.doi.org/10.1371/journal.pone.0134920
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