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Novel Bread Wheat Lines Enriched in Carotenoids Carrying Hordeum chilense Chromosome Arms in the ph1b Background

The use of crop wild relative species to improve major crops performance is well established. Hordeum chilense has a high potential as a genetic donor to increase the carotenoid content of wheat. Crosses between the 7H(ch) H. chilense substitution lines in wheat and the wheat pairing homoeologous1b...

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Autores principales: Rey, María-Dolores, Calderón, María-Carmen, Rodrigo, María Jesús, Zacarías, Lorenzo, Alós, Enriqueta, Prieto, Pilar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524710/
https://www.ncbi.nlm.nih.gov/pubmed/26241856
http://dx.doi.org/10.1371/journal.pone.0134598
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author Rey, María-Dolores
Calderón, María-Carmen
Rodrigo, María Jesús
Zacarías, Lorenzo
Alós, Enriqueta
Prieto, Pilar
author_facet Rey, María-Dolores
Calderón, María-Carmen
Rodrigo, María Jesús
Zacarías, Lorenzo
Alós, Enriqueta
Prieto, Pilar
author_sort Rey, María-Dolores
collection PubMed
description The use of crop wild relative species to improve major crops performance is well established. Hordeum chilense has a high potential as a genetic donor to increase the carotenoid content of wheat. Crosses between the 7H(ch) H. chilense substitution lines in wheat and the wheat pairing homoeologous1b (ph1b) mutant allowed the development of wheat-H. chilense translocation lines for both 7H(ch)α and 7H(ch)β chromosome arms in the wheat background. These translocation lines were characterized by in situ hybridization and using molecular markers. In addition, reverse phase chromatography (HPLC) analysis was carried out to evaluate the carotenoid content and both 7H(ch)α∙7AL and 7AS∙7H(ch)β disomic translocation lines. The carotenoid content in 7H(ch)α∙7AL and 7AS∙7H(ch)β disomic translocation lines was higher than the wheat-7H(ch) addition line and double amount of carotenoids than the wheat itself. A proteomic analysis confirmed that the presence of chromosome 7H(ch) introgressions in wheat scarcely altered the proteomic profile of the wheat flour. The Psy1 (Phytoene Synthase1) gene, which is the first committed step in the carotenoid biosynthetic pathway, was also cytogenetically mapped on the 7H(ch)α chromosome arm. These new wheat-H. chilense translocation lines can be used as a powerful tool in wheat breeding programs to enrich the diet in bioactive compounds.
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spelling pubmed-45247102015-08-06 Novel Bread Wheat Lines Enriched in Carotenoids Carrying Hordeum chilense Chromosome Arms in the ph1b Background Rey, María-Dolores Calderón, María-Carmen Rodrigo, María Jesús Zacarías, Lorenzo Alós, Enriqueta Prieto, Pilar PLoS One Research Article The use of crop wild relative species to improve major crops performance is well established. Hordeum chilense has a high potential as a genetic donor to increase the carotenoid content of wheat. Crosses between the 7H(ch) H. chilense substitution lines in wheat and the wheat pairing homoeologous1b (ph1b) mutant allowed the development of wheat-H. chilense translocation lines for both 7H(ch)α and 7H(ch)β chromosome arms in the wheat background. These translocation lines were characterized by in situ hybridization and using molecular markers. In addition, reverse phase chromatography (HPLC) analysis was carried out to evaluate the carotenoid content and both 7H(ch)α∙7AL and 7AS∙7H(ch)β disomic translocation lines. The carotenoid content in 7H(ch)α∙7AL and 7AS∙7H(ch)β disomic translocation lines was higher than the wheat-7H(ch) addition line and double amount of carotenoids than the wheat itself. A proteomic analysis confirmed that the presence of chromosome 7H(ch) introgressions in wheat scarcely altered the proteomic profile of the wheat flour. The Psy1 (Phytoene Synthase1) gene, which is the first committed step in the carotenoid biosynthetic pathway, was also cytogenetically mapped on the 7H(ch)α chromosome arm. These new wheat-H. chilense translocation lines can be used as a powerful tool in wheat breeding programs to enrich the diet in bioactive compounds. Public Library of Science 2015-08-04 /pmc/articles/PMC4524710/ /pubmed/26241856 http://dx.doi.org/10.1371/journal.pone.0134598 Text en © 2015 Rey et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Rey, María-Dolores
Calderón, María-Carmen
Rodrigo, María Jesús
Zacarías, Lorenzo
Alós, Enriqueta
Prieto, Pilar
Novel Bread Wheat Lines Enriched in Carotenoids Carrying Hordeum chilense Chromosome Arms in the ph1b Background
title Novel Bread Wheat Lines Enriched in Carotenoids Carrying Hordeum chilense Chromosome Arms in the ph1b Background
title_full Novel Bread Wheat Lines Enriched in Carotenoids Carrying Hordeum chilense Chromosome Arms in the ph1b Background
title_fullStr Novel Bread Wheat Lines Enriched in Carotenoids Carrying Hordeum chilense Chromosome Arms in the ph1b Background
title_full_unstemmed Novel Bread Wheat Lines Enriched in Carotenoids Carrying Hordeum chilense Chromosome Arms in the ph1b Background
title_short Novel Bread Wheat Lines Enriched in Carotenoids Carrying Hordeum chilense Chromosome Arms in the ph1b Background
title_sort novel bread wheat lines enriched in carotenoids carrying hordeum chilense chromosome arms in the ph1b background
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524710/
https://www.ncbi.nlm.nih.gov/pubmed/26241856
http://dx.doi.org/10.1371/journal.pone.0134598
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