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Formation of the hindgut cuticular lining during embryonic development of Porcellio scaber (Crustacea, Isopoda)
Abstract. The hindgut and foregut in terrestrial isopod crustaceans are ectodermal parts of the digestive system and are lined by cuticle, an apical extracellular matrix secreted by epithelial cells. Morphogenesis of the digestive system was reported in previous studies, but differentiation of the g...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pensoft Publishers
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525038/ https://www.ncbi.nlm.nih.gov/pubmed/26261443 http://dx.doi.org/10.3897/zookeys.515.9468 |
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author | Mrak, Polona Bogataj, Urban Štrus, Jasna Žnidaršič, Nada |
author_facet | Mrak, Polona Bogataj, Urban Štrus, Jasna Žnidaršič, Nada |
author_sort | Mrak, Polona |
collection | PubMed |
description | Abstract. The hindgut and foregut in terrestrial isopod crustaceans are ectodermal parts of the digestive system and are lined by cuticle, an apical extracellular matrix secreted by epithelial cells. Morphogenesis of the digestive system was reported in previous studies, but differentiation of the gut cuticle was not followed in detail. This study is focused on ultrastructural analyses of hindgut apical matrices and cuticle in selected intramarsupial developmental stages of the terrestrial isopod Porcellio scaber in comparison to adult animals to obtain data on the hindgut cuticular lining differentiation. Our results show that in late embryos of stages 16 and 18 the apical matrix in the hindgut consists of loose material overlaid by a thin intensely ruffled electron dense lamina facing the lumen. The ultrastructural resemblance to the embryonic epidermal matrices described in several arthropods suggests a common principle in chitinous matrix differentiation. The hindgut matrix in the prehatching embryo of stage 19 shows characteristics of the hindgut cuticle, specifically alignment to the apical epithelial surface and a prominent electron dense layer of epicuticle. In the preceding embryonic stage – stage 18 – an electron dense lamina, closely apposed to the apical cell membrane, is evident and is considered as the first epicuticle formation. In marsupial mancae the advanced features of the hindgut cuticle and epithelium are evident: a more prominent epicuticular layer, formation of cuticular spines and an extensive apical labyrinth. In comparison to the hindgut cuticle of adults, the hindgut cuticle of marsupial manca and in particular the electron dense epicuticular layer are much thinner and the difference between cuticle architecture in the anterior chamber and in the papillate region is not yet distinguishable. Differences from the hindgut cuticle in adults imply not fully developed structure and function of the hindgut cuticle in marsupial manca, possibly related also to different environments, as mancae develop in marsupial fluid. Bacteria, evenly distributed within the homogenous electron dense material in the hindgut lumen, were observed only in one specimen of early marsupial manca. The morphological features of gut cuticle renewal are evident in the late marsupial mancae, and are similar to those observed in the exoskeleton. |
format | Online Article Text |
id | pubmed-4525038 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Pensoft Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-45250382015-08-10 Formation of the hindgut cuticular lining during embryonic development of Porcellio scaber (Crustacea, Isopoda) Mrak, Polona Bogataj, Urban Štrus, Jasna Žnidaršič, Nada Zookeys Research Article Abstract. The hindgut and foregut in terrestrial isopod crustaceans are ectodermal parts of the digestive system and are lined by cuticle, an apical extracellular matrix secreted by epithelial cells. Morphogenesis of the digestive system was reported in previous studies, but differentiation of the gut cuticle was not followed in detail. This study is focused on ultrastructural analyses of hindgut apical matrices and cuticle in selected intramarsupial developmental stages of the terrestrial isopod Porcellio scaber in comparison to adult animals to obtain data on the hindgut cuticular lining differentiation. Our results show that in late embryos of stages 16 and 18 the apical matrix in the hindgut consists of loose material overlaid by a thin intensely ruffled electron dense lamina facing the lumen. The ultrastructural resemblance to the embryonic epidermal matrices described in several arthropods suggests a common principle in chitinous matrix differentiation. The hindgut matrix in the prehatching embryo of stage 19 shows characteristics of the hindgut cuticle, specifically alignment to the apical epithelial surface and a prominent electron dense layer of epicuticle. In the preceding embryonic stage – stage 18 – an electron dense lamina, closely apposed to the apical cell membrane, is evident and is considered as the first epicuticle formation. In marsupial mancae the advanced features of the hindgut cuticle and epithelium are evident: a more prominent epicuticular layer, formation of cuticular spines and an extensive apical labyrinth. In comparison to the hindgut cuticle of adults, the hindgut cuticle of marsupial manca and in particular the electron dense epicuticular layer are much thinner and the difference between cuticle architecture in the anterior chamber and in the papillate region is not yet distinguishable. Differences from the hindgut cuticle in adults imply not fully developed structure and function of the hindgut cuticle in marsupial manca, possibly related also to different environments, as mancae develop in marsupial fluid. Bacteria, evenly distributed within the homogenous electron dense material in the hindgut lumen, were observed only in one specimen of early marsupial manca. The morphological features of gut cuticle renewal are evident in the late marsupial mancae, and are similar to those observed in the exoskeleton. Pensoft Publishers 2015-07-30 /pmc/articles/PMC4525038/ /pubmed/26261443 http://dx.doi.org/10.3897/zookeys.515.9468 Text en Polona Mrak, Urban Bogataj, Jasna Štrus, Nada Žnidaršič http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Mrak, Polona Bogataj, Urban Štrus, Jasna Žnidaršič, Nada Formation of the hindgut cuticular lining during embryonic development of Porcellio scaber (Crustacea, Isopoda) |
title | Formation of the hindgut cuticular lining during embryonic development of Porcellio
scaber (Crustacea, Isopoda) |
title_full | Formation of the hindgut cuticular lining during embryonic development of Porcellio
scaber (Crustacea, Isopoda) |
title_fullStr | Formation of the hindgut cuticular lining during embryonic development of Porcellio
scaber (Crustacea, Isopoda) |
title_full_unstemmed | Formation of the hindgut cuticular lining during embryonic development of Porcellio
scaber (Crustacea, Isopoda) |
title_short | Formation of the hindgut cuticular lining during embryonic development of Porcellio
scaber (Crustacea, Isopoda) |
title_sort | formation of the hindgut cuticular lining during embryonic development of porcellio
scaber (crustacea, isopoda) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525038/ https://www.ncbi.nlm.nih.gov/pubmed/26261443 http://dx.doi.org/10.3897/zookeys.515.9468 |
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