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MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis
Currently microorganisms are best identified using 16S rRNA and 18S rRNA gene sequencing. However, in recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potential tool for microbial identification and diagnosis. During the MALDI-...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525378/ https://www.ncbi.nlm.nih.gov/pubmed/26300860 http://dx.doi.org/10.3389/fmicb.2015.00791 |
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author | Singhal, Neelja Kumar, Manish Kanaujia, Pawan K. Virdi, Jugsharan S. |
author_facet | Singhal, Neelja Kumar, Manish Kanaujia, Pawan K. Virdi, Jugsharan S. |
author_sort | Singhal, Neelja |
collection | PubMed |
description | Currently microorganisms are best identified using 16S rRNA and 18S rRNA gene sequencing. However, in recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potential tool for microbial identification and diagnosis. During the MALDI-TOF MS process, microbes are identified using either intact cells or cell extracts. The process is rapid, sensitive, and economical in terms of both labor and costs involved. The technology has been readily imbibed by microbiologists who have reported usage of MALDI-TOF MS for a number of purposes like, microbial identification and strain typing, epidemiological studies, detection of biological warfare agents, detection of water- and food-borne pathogens, detection of antibiotic resistance and detection of blood and urinary tract pathogens etc. The limitation of the technology is that identification of new isolates is possible only if the spectral database contains peptide mass fingerprints of the type strains of specific genera/species/subspecies/strains. This review provides an overview of the status and recent applications of mass spectrometry for microbial identification. It also explores the usefulness of this exciting new technology for diagnosis of diseases caused by bacteria, viruses, and fungi. |
format | Online Article Text |
id | pubmed-4525378 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-45253782015-08-21 MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis Singhal, Neelja Kumar, Manish Kanaujia, Pawan K. Virdi, Jugsharan S. Front Microbiol Microbiology Currently microorganisms are best identified using 16S rRNA and 18S rRNA gene sequencing. However, in recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potential tool for microbial identification and diagnosis. During the MALDI-TOF MS process, microbes are identified using either intact cells or cell extracts. The process is rapid, sensitive, and economical in terms of both labor and costs involved. The technology has been readily imbibed by microbiologists who have reported usage of MALDI-TOF MS for a number of purposes like, microbial identification and strain typing, epidemiological studies, detection of biological warfare agents, detection of water- and food-borne pathogens, detection of antibiotic resistance and detection of blood and urinary tract pathogens etc. The limitation of the technology is that identification of new isolates is possible only if the spectral database contains peptide mass fingerprints of the type strains of specific genera/species/subspecies/strains. This review provides an overview of the status and recent applications of mass spectrometry for microbial identification. It also explores the usefulness of this exciting new technology for diagnosis of diseases caused by bacteria, viruses, and fungi. Frontiers Media S.A. 2015-08-05 /pmc/articles/PMC4525378/ /pubmed/26300860 http://dx.doi.org/10.3389/fmicb.2015.00791 Text en Copyright © 2015 Singhal, Kumar, Kanaujia and Virdi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Singhal, Neelja Kumar, Manish Kanaujia, Pawan K. Virdi, Jugsharan S. MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis |
title | MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis |
title_full | MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis |
title_fullStr | MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis |
title_full_unstemmed | MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis |
title_short | MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis |
title_sort | maldi-tof mass spectrometry: an emerging technology for microbial identification and diagnosis |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525378/ https://www.ncbi.nlm.nih.gov/pubmed/26300860 http://dx.doi.org/10.3389/fmicb.2015.00791 |
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