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Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus

Newly designed primers targeting rbcL (CO(2) fixation), psbA (photosystem II) and rnpB (reference) genes were used in qRT-PCR assays to assess the photosynthetic capability of natural communities of Prochlorococcus, the most abundant photosynthetic organism on Earth and a major contributor to primar...

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Autores principales: Fernández-Pinos, María-Carmen, Casado, Marta, Caballero, Gemma, Zinser, Erik R., Dachs, Jordi, Piña, Benjamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4526520/
https://www.ncbi.nlm.nih.gov/pubmed/26244890
http://dx.doi.org/10.1371/journal.pone.0133207
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author Fernández-Pinos, María-Carmen
Casado, Marta
Caballero, Gemma
Zinser, Erik R.
Dachs, Jordi
Piña, Benjamin
author_facet Fernández-Pinos, María-Carmen
Casado, Marta
Caballero, Gemma
Zinser, Erik R.
Dachs, Jordi
Piña, Benjamin
author_sort Fernández-Pinos, María-Carmen
collection PubMed
description Newly designed primers targeting rbcL (CO(2) fixation), psbA (photosystem II) and rnpB (reference) genes were used in qRT-PCR assays to assess the photosynthetic capability of natural communities of Prochlorococcus, the most abundant photosynthetic organism on Earth and a major contributor to primary production in oligotrophic oceans. After optimizing sample collection methodology, we analyzed a total of 62 stations from the Malaspina 2010 circumnavigation (including Atlantic, Pacific and Indian Oceans) at three different depths. Sequence and quantitative analyses of the corresponding amplicons showed the presence of high-light (HL) and low-light (LL) Prochlorococcus clades in essentially all 182 samples, with a largely uniform stratification of LL and HL sequences. Synechococcus cross-amplifications were detected by the taxon-specific melting temperatures of the amplicons. Laboratory exposure of Prochlorococcus MED4 (HL) and MIT9313 (LL) strains to organic pollutants (PAHs and organochlorine compounds) showed a decrease of rbcL transcript abundances, and of the rbcL to psbA ratios for both strains. We propose this technique as a convenient assay to evaluate effects of environmental stressors, including pollution, on the oceanic Prochlorococcus photosynthetic function.
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spelling pubmed-45265202015-08-12 Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus Fernández-Pinos, María-Carmen Casado, Marta Caballero, Gemma Zinser, Erik R. Dachs, Jordi Piña, Benjamin PLoS One Research Article Newly designed primers targeting rbcL (CO(2) fixation), psbA (photosystem II) and rnpB (reference) genes were used in qRT-PCR assays to assess the photosynthetic capability of natural communities of Prochlorococcus, the most abundant photosynthetic organism on Earth and a major contributor to primary production in oligotrophic oceans. After optimizing sample collection methodology, we analyzed a total of 62 stations from the Malaspina 2010 circumnavigation (including Atlantic, Pacific and Indian Oceans) at three different depths. Sequence and quantitative analyses of the corresponding amplicons showed the presence of high-light (HL) and low-light (LL) Prochlorococcus clades in essentially all 182 samples, with a largely uniform stratification of LL and HL sequences. Synechococcus cross-amplifications were detected by the taxon-specific melting temperatures of the amplicons. Laboratory exposure of Prochlorococcus MED4 (HL) and MIT9313 (LL) strains to organic pollutants (PAHs and organochlorine compounds) showed a decrease of rbcL transcript abundances, and of the rbcL to psbA ratios for both strains. We propose this technique as a convenient assay to evaluate effects of environmental stressors, including pollution, on the oceanic Prochlorococcus photosynthetic function. Public Library of Science 2015-08-05 /pmc/articles/PMC4526520/ /pubmed/26244890 http://dx.doi.org/10.1371/journal.pone.0133207 Text en © 2015 Fernández-Pinos et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fernández-Pinos, María-Carmen
Casado, Marta
Caballero, Gemma
Zinser, Erik R.
Dachs, Jordi
Piña, Benjamin
Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus
title Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus
title_full Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus
title_fullStr Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus
title_full_unstemmed Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus
title_short Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus
title_sort clade-specific quantitative analysis of photosynthetic gene expression in prochlorococcus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4526520/
https://www.ncbi.nlm.nih.gov/pubmed/26244890
http://dx.doi.org/10.1371/journal.pone.0133207
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