Cargando…

Heterozygous Mapping Strategy (HetMappS) for High Resolution Genotyping-By-Sequencing Markers: A Case Study in Grapevine

Genotyping by sequencing (GBS) provides opportunities to generate high-resolution genetic maps at a low genotyping cost, but for highly heterozygous species, missing data and heterozygote undercalling complicate the creation of GBS genetic maps. To overcome these issues, we developed a publicly avai...

Descripción completa

Detalles Bibliográficos
Autores principales: Hyma, Katie E., Barba, Paola, Wang, Minghui, Londo, Jason P., Acharya, Charlotte B., Mitchell, Sharon E., Sun, Qi, Reisch, Bruce, Cadle-Davidson, Lance
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4526651/
https://www.ncbi.nlm.nih.gov/pubmed/26244767
http://dx.doi.org/10.1371/journal.pone.0134880
_version_ 1782384447976374272
author Hyma, Katie E.
Barba, Paola
Wang, Minghui
Londo, Jason P.
Acharya, Charlotte B.
Mitchell, Sharon E.
Sun, Qi
Reisch, Bruce
Cadle-Davidson, Lance
author_facet Hyma, Katie E.
Barba, Paola
Wang, Minghui
Londo, Jason P.
Acharya, Charlotte B.
Mitchell, Sharon E.
Sun, Qi
Reisch, Bruce
Cadle-Davidson, Lance
author_sort Hyma, Katie E.
collection PubMed
description Genotyping by sequencing (GBS) provides opportunities to generate high-resolution genetic maps at a low genotyping cost, but for highly heterozygous species, missing data and heterozygote undercalling complicate the creation of GBS genetic maps. To overcome these issues, we developed a publicly available, modular approach called HetMappS, which functions independently of parental genotypes and corrects for genotyping errors associated with heterozygosity. For linkage group formation, HetMappS includes both a reference-guided synteny pipeline and a reference-independent de novo pipeline. The de novo pipeline can be utilized for under-characterized or high diversity families that lack an appropriate reference. We applied both HetMappS pipelines in five half-sib F(1) families involving genetically diverse Vitis spp. Starting with at least 116,466 putative SNPs per family, the HetMappS pipelines identified 10,440 to 17,267 phased pseudo-testcross (Pt) markers and generated high-confidence maps. Pt marker density exceeded crossover resolution in all cases; up to 5,560 non-redundant markers were used to generate parental maps ranging from 1,047 cM to 1,696 cM. The number of markers used was strongly correlated with family size in both de novo and synteny maps (r = 0.92 and 0.91, respectively). Comparisons between allele and tag frequencies suggested that many markers were in tandem repeats and mapped as single loci, while markers in regions of more than two repeats were removed during map curation. Both pipelines generated similar genetic maps, and genetic order was strongly correlated with the reference genome physical order in all cases. Independently created genetic maps from shared parents exhibited nearly identical results. Flower sex was mapped in three families and correctly localized to the known sex locus in all cases. The HetMappS pipeline could have wide application for genetic mapping in highly heterozygous species, and its modularity provides opportunities to adapt portions of the pipeline to other family types, genotyping technologies or applications.
format Online
Article
Text
id pubmed-4526651
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-45266512015-08-12 Heterozygous Mapping Strategy (HetMappS) for High Resolution Genotyping-By-Sequencing Markers: A Case Study in Grapevine Hyma, Katie E. Barba, Paola Wang, Minghui Londo, Jason P. Acharya, Charlotte B. Mitchell, Sharon E. Sun, Qi Reisch, Bruce Cadle-Davidson, Lance PLoS One Research Article Genotyping by sequencing (GBS) provides opportunities to generate high-resolution genetic maps at a low genotyping cost, but for highly heterozygous species, missing data and heterozygote undercalling complicate the creation of GBS genetic maps. To overcome these issues, we developed a publicly available, modular approach called HetMappS, which functions independently of parental genotypes and corrects for genotyping errors associated with heterozygosity. For linkage group formation, HetMappS includes both a reference-guided synteny pipeline and a reference-independent de novo pipeline. The de novo pipeline can be utilized for under-characterized or high diversity families that lack an appropriate reference. We applied both HetMappS pipelines in five half-sib F(1) families involving genetically diverse Vitis spp. Starting with at least 116,466 putative SNPs per family, the HetMappS pipelines identified 10,440 to 17,267 phased pseudo-testcross (Pt) markers and generated high-confidence maps. Pt marker density exceeded crossover resolution in all cases; up to 5,560 non-redundant markers were used to generate parental maps ranging from 1,047 cM to 1,696 cM. The number of markers used was strongly correlated with family size in both de novo and synteny maps (r = 0.92 and 0.91, respectively). Comparisons between allele and tag frequencies suggested that many markers were in tandem repeats and mapped as single loci, while markers in regions of more than two repeats were removed during map curation. Both pipelines generated similar genetic maps, and genetic order was strongly correlated with the reference genome physical order in all cases. Independently created genetic maps from shared parents exhibited nearly identical results. Flower sex was mapped in three families and correctly localized to the known sex locus in all cases. The HetMappS pipeline could have wide application for genetic mapping in highly heterozygous species, and its modularity provides opportunities to adapt portions of the pipeline to other family types, genotyping technologies or applications. Public Library of Science 2015-08-05 /pmc/articles/PMC4526651/ /pubmed/26244767 http://dx.doi.org/10.1371/journal.pone.0134880 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Hyma, Katie E.
Barba, Paola
Wang, Minghui
Londo, Jason P.
Acharya, Charlotte B.
Mitchell, Sharon E.
Sun, Qi
Reisch, Bruce
Cadle-Davidson, Lance
Heterozygous Mapping Strategy (HetMappS) for High Resolution Genotyping-By-Sequencing Markers: A Case Study in Grapevine
title Heterozygous Mapping Strategy (HetMappS) for High Resolution Genotyping-By-Sequencing Markers: A Case Study in Grapevine
title_full Heterozygous Mapping Strategy (HetMappS) for High Resolution Genotyping-By-Sequencing Markers: A Case Study in Grapevine
title_fullStr Heterozygous Mapping Strategy (HetMappS) for High Resolution Genotyping-By-Sequencing Markers: A Case Study in Grapevine
title_full_unstemmed Heterozygous Mapping Strategy (HetMappS) for High Resolution Genotyping-By-Sequencing Markers: A Case Study in Grapevine
title_short Heterozygous Mapping Strategy (HetMappS) for High Resolution Genotyping-By-Sequencing Markers: A Case Study in Grapevine
title_sort heterozygous mapping strategy (hetmapps) for high resolution genotyping-by-sequencing markers: a case study in grapevine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4526651/
https://www.ncbi.nlm.nih.gov/pubmed/26244767
http://dx.doi.org/10.1371/journal.pone.0134880
work_keys_str_mv AT hymakatiee heterozygousmappingstrategyhetmappsforhighresolutiongenotypingbysequencingmarkersacasestudyingrapevine
AT barbapaola heterozygousmappingstrategyhetmappsforhighresolutiongenotypingbysequencingmarkersacasestudyingrapevine
AT wangminghui heterozygousmappingstrategyhetmappsforhighresolutiongenotypingbysequencingmarkersacasestudyingrapevine
AT londojasonp heterozygousmappingstrategyhetmappsforhighresolutiongenotypingbysequencingmarkersacasestudyingrapevine
AT acharyacharlotteb heterozygousmappingstrategyhetmappsforhighresolutiongenotypingbysequencingmarkersacasestudyingrapevine
AT mitchellsharone heterozygousmappingstrategyhetmappsforhighresolutiongenotypingbysequencingmarkersacasestudyingrapevine
AT sunqi heterozygousmappingstrategyhetmappsforhighresolutiongenotypingbysequencingmarkersacasestudyingrapevine
AT reischbruce heterozygousmappingstrategyhetmappsforhighresolutiongenotypingbysequencingmarkersacasestudyingrapevine
AT cadledavidsonlance heterozygousmappingstrategyhetmappsforhighresolutiongenotypingbysequencingmarkersacasestudyingrapevine