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Antibacterial activity of ethanolic extract and compounds from fruits of Tectona grandis (Verbenaceae)
BACKGROUND: Well known as teak, Tectona grandis is widely used in African folk medicine for its pharmacological relevance. In Cameroon, this species is a reputed laxative in the Northern Region while in the Western Region, it is used in the treatment of skin diseases and diarrhoea. MATERIALS AND MET...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527215/ https://www.ncbi.nlm.nih.gov/pubmed/26245866 http://dx.doi.org/10.1186/s12906-015-0790-5 |
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author | Bitchagno, Gabin Thierry M. Sama Fonkeng, Leonard Kopa, Théodora K. Tala, Michel F. Kamdem Wabo, Hippolyte Tume, Christopher B. Tane, Pierre Kuiate, Jules-Roger |
author_facet | Bitchagno, Gabin Thierry M. Sama Fonkeng, Leonard Kopa, Théodora K. Tala, Michel F. Kamdem Wabo, Hippolyte Tume, Christopher B. Tane, Pierre Kuiate, Jules-Roger |
author_sort | Bitchagno, Gabin Thierry M. |
collection | PubMed |
description | BACKGROUND: Well known as teak, Tectona grandis is widely used in African folk medicine for its pharmacological relevance. In Cameroon, this species is a reputed laxative in the Northern Region while in the Western Region, it is used in the treatment of skin diseases and diarrhoea. MATERIALS AND METHODS: Separation and isolation of compounds were performed using different chromatographic methods while their structures were elucidated by spectroscopic techniques including MS and NMR, and by comparison of data with those reported in the literature. Isolated compounds as well as crude ethanol extract were tested for their antibacterial activities using broth micro-dilution method against four Gram negative bacteria strains Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (PA 01), Klebsiella pneumonia (ATCC 11296) and Escherichia aerogenes (ATCC 13048). RESULTS: Three known compounds were isolated, including two quinones and one triterpene. They were identified as tectograndone (1), 6-methyl-1,4-dihydroxyanthraquinone (2), and 2β-hydroxyursolic acid (3) respectively. Crude ethanol extract showed good activity against the bacteria strains tested with MIC of 64–256 μg/mL. Among the isolated metabolites, 6-methyl-1,4-dihydroxyanthraquinone exhibited a strong activity against Escherichia aerogenes with MIC of 16 μg/mL, while tectograndone showed a moderate activity against Escherichia coli with MIC of 32 μg/mL. The antibacterial screening of the fruits of this plant as well as that of compounds 1 and 2 is reported herein for the first time. CONCLUSION: The research work presented here shows that Tectona grandis fruits possess compounds which could be developed in the treatment of bacterial diseases. |
format | Online Article Text |
id | pubmed-4527215 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-45272152015-08-07 Antibacterial activity of ethanolic extract and compounds from fruits of Tectona grandis (Verbenaceae) Bitchagno, Gabin Thierry M. Sama Fonkeng, Leonard Kopa, Théodora K. Tala, Michel F. Kamdem Wabo, Hippolyte Tume, Christopher B. Tane, Pierre Kuiate, Jules-Roger BMC Complement Altern Med Research Article BACKGROUND: Well known as teak, Tectona grandis is widely used in African folk medicine for its pharmacological relevance. In Cameroon, this species is a reputed laxative in the Northern Region while in the Western Region, it is used in the treatment of skin diseases and diarrhoea. MATERIALS AND METHODS: Separation and isolation of compounds were performed using different chromatographic methods while their structures were elucidated by spectroscopic techniques including MS and NMR, and by comparison of data with those reported in the literature. Isolated compounds as well as crude ethanol extract were tested for their antibacterial activities using broth micro-dilution method against four Gram negative bacteria strains Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (PA 01), Klebsiella pneumonia (ATCC 11296) and Escherichia aerogenes (ATCC 13048). RESULTS: Three known compounds were isolated, including two quinones and one triterpene. They were identified as tectograndone (1), 6-methyl-1,4-dihydroxyanthraquinone (2), and 2β-hydroxyursolic acid (3) respectively. Crude ethanol extract showed good activity against the bacteria strains tested with MIC of 64–256 μg/mL. Among the isolated metabolites, 6-methyl-1,4-dihydroxyanthraquinone exhibited a strong activity against Escherichia aerogenes with MIC of 16 μg/mL, while tectograndone showed a moderate activity against Escherichia coli with MIC of 32 μg/mL. The antibacterial screening of the fruits of this plant as well as that of compounds 1 and 2 is reported herein for the first time. CONCLUSION: The research work presented here shows that Tectona grandis fruits possess compounds which could be developed in the treatment of bacterial diseases. BioMed Central 2015-08-06 /pmc/articles/PMC4527215/ /pubmed/26245866 http://dx.doi.org/10.1186/s12906-015-0790-5 Text en © Bitchagno et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Bitchagno, Gabin Thierry M. Sama Fonkeng, Leonard Kopa, Théodora K. Tala, Michel F. Kamdem Wabo, Hippolyte Tume, Christopher B. Tane, Pierre Kuiate, Jules-Roger Antibacterial activity of ethanolic extract and compounds from fruits of Tectona grandis (Verbenaceae) |
title | Antibacterial activity of ethanolic extract and compounds from fruits of Tectona grandis (Verbenaceae) |
title_full | Antibacterial activity of ethanolic extract and compounds from fruits of Tectona grandis (Verbenaceae) |
title_fullStr | Antibacterial activity of ethanolic extract and compounds from fruits of Tectona grandis (Verbenaceae) |
title_full_unstemmed | Antibacterial activity of ethanolic extract and compounds from fruits of Tectona grandis (Verbenaceae) |
title_short | Antibacterial activity of ethanolic extract and compounds from fruits of Tectona grandis (Verbenaceae) |
title_sort | antibacterial activity of ethanolic extract and compounds from fruits of tectona grandis (verbenaceae) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527215/ https://www.ncbi.nlm.nih.gov/pubmed/26245866 http://dx.doi.org/10.1186/s12906-015-0790-5 |
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