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Sun1 deficiency leads to cerebellar ataxia in mice
Migration and organization of the nucleus are essential for the proliferation and differentiation of cells, including neurons. However, the relationship between the positioning of the nucleus and cellular morphogenesis remains poorly understood. Inherited recessive cerebellar ataxia has been attribu...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527285/ https://www.ncbi.nlm.nih.gov/pubmed/26035387 http://dx.doi.org/10.1242/dmm.019240 |
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author | Wang, Jing-Ya Yu, I.-Shing Huang, Chien-Chi Chen, Chia-Yen Wang, Wan-Ping Lin, Shu-Wha Jeang, Kuan-Teh Chi, Ya-Hui |
author_facet | Wang, Jing-Ya Yu, I.-Shing Huang, Chien-Chi Chen, Chia-Yen Wang, Wan-Ping Lin, Shu-Wha Jeang, Kuan-Teh Chi, Ya-Hui |
author_sort | Wang, Jing-Ya |
collection | PubMed |
description | Migration and organization of the nucleus are essential for the proliferation and differentiation of cells, including neurons. However, the relationship between the positioning of the nucleus and cellular morphogenesis remains poorly understood. Inherited recessive cerebellar ataxia has been attributed to mutations in SYNE1, a component of the linker of nucleoskeleton and cytoskeleton (LINC) complex. Regardless, Syne1-mutant mice present with normal cerebellar development. The Sad1-Unc-84 homology (SUN)-domain proteins are located at the inner nuclear membrane and recruit Syne proteins through the KASH domain to the outer nuclear membrane. Here, we report an unrecognized contribution of Sun1 and Sun2 to the postnatal development of murine cerebellum. Mice depleted of Sun1 showed a marked reduction in the cerebellar volume, and this phenotype is exacerbated with additional loss of a Sun2 allele. Consistent with these histological changes, Sun1(−/−) and Sun1(−/−)Sun2(+/−) mice exhibited defective motor coordination. Results of immunohistochemical analyses suggested that Sun1 is highly expressed in Purkinje cells and recruits Syne2 to the periphery of the nucleus. Approximately 33% of Purkinje cells in Sun1(−/−) mice and 66% of Purkinje cells in Sun1(−/−)Sun2(+/−) mice were absent from the surface of the internal granule layer (IGL), whereas the proliferation and migration of granule neurons were unaffected. Furthermore, the Sun1(−/−)Sun2(+/−) Purkinje cells exhibited retarded primary dendrite specification, reduced dendritic complexity and aberrant patterning of synapses. Our findings reveal a cell-type-specific role for Sun1 and Sun2 in nucleokinesis during cerebellar development, and we propose the use of Sun-deficient mice as a model for studying cerebellar ataxia that is associated with mutation of human SYNE genes or loss of Purkinje cells. |
format | Online Article Text |
id | pubmed-4527285 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Company of Biologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-45272852015-09-03 Sun1 deficiency leads to cerebellar ataxia in mice Wang, Jing-Ya Yu, I.-Shing Huang, Chien-Chi Chen, Chia-Yen Wang, Wan-Ping Lin, Shu-Wha Jeang, Kuan-Teh Chi, Ya-Hui Dis Model Mech Research Article Migration and organization of the nucleus are essential for the proliferation and differentiation of cells, including neurons. However, the relationship between the positioning of the nucleus and cellular morphogenesis remains poorly understood. Inherited recessive cerebellar ataxia has been attributed to mutations in SYNE1, a component of the linker of nucleoskeleton and cytoskeleton (LINC) complex. Regardless, Syne1-mutant mice present with normal cerebellar development. The Sad1-Unc-84 homology (SUN)-domain proteins are located at the inner nuclear membrane and recruit Syne proteins through the KASH domain to the outer nuclear membrane. Here, we report an unrecognized contribution of Sun1 and Sun2 to the postnatal development of murine cerebellum. Mice depleted of Sun1 showed a marked reduction in the cerebellar volume, and this phenotype is exacerbated with additional loss of a Sun2 allele. Consistent with these histological changes, Sun1(−/−) and Sun1(−/−)Sun2(+/−) mice exhibited defective motor coordination. Results of immunohistochemical analyses suggested that Sun1 is highly expressed in Purkinje cells and recruits Syne2 to the periphery of the nucleus. Approximately 33% of Purkinje cells in Sun1(−/−) mice and 66% of Purkinje cells in Sun1(−/−)Sun2(+/−) mice were absent from the surface of the internal granule layer (IGL), whereas the proliferation and migration of granule neurons were unaffected. Furthermore, the Sun1(−/−)Sun2(+/−) Purkinje cells exhibited retarded primary dendrite specification, reduced dendritic complexity and aberrant patterning of synapses. Our findings reveal a cell-type-specific role for Sun1 and Sun2 in nucleokinesis during cerebellar development, and we propose the use of Sun-deficient mice as a model for studying cerebellar ataxia that is associated with mutation of human SYNE genes or loss of Purkinje cells. The Company of Biologists 2015-08-01 /pmc/articles/PMC4527285/ /pubmed/26035387 http://dx.doi.org/10.1242/dmm.019240 Text en © 2015. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Wang, Jing-Ya Yu, I.-Shing Huang, Chien-Chi Chen, Chia-Yen Wang, Wan-Ping Lin, Shu-Wha Jeang, Kuan-Teh Chi, Ya-Hui Sun1 deficiency leads to cerebellar ataxia in mice |
title | Sun1 deficiency leads to cerebellar ataxia in mice |
title_full | Sun1 deficiency leads to cerebellar ataxia in mice |
title_fullStr | Sun1 deficiency leads to cerebellar ataxia in mice |
title_full_unstemmed | Sun1 deficiency leads to cerebellar ataxia in mice |
title_short | Sun1 deficiency leads to cerebellar ataxia in mice |
title_sort | sun1 deficiency leads to cerebellar ataxia in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527285/ https://www.ncbi.nlm.nih.gov/pubmed/26035387 http://dx.doi.org/10.1242/dmm.019240 |
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