Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis

Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomes was detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL) without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to 3 EcoRI and HindIII fragments in...

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Autores principales: MIURA, Saori, HORIUCHI, Noriyuki, MATSUMOTO, Kotaro, KOBAYASHI, Yoshiyasu, KAWAZU, Shin-ichiro, INOKUMA, Hisashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Internal Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527516/
https://www.ncbi.nlm.nih.gov/pubmed/25766769
http://dx.doi.org/10.1292/jvms.14-0591
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author MIURA, Saori
HORIUCHI, Noriyuki
MATSUMOTO, Kotaro
KOBAYASHI, Yoshiyasu
KAWAZU, Shin-ichiro
INOKUMA, Hisashi
author_facet MIURA, Saori
HORIUCHI, Noriyuki
MATSUMOTO, Kotaro
KOBAYASHI, Yoshiyasu
KAWAZU, Shin-ichiro
INOKUMA, Hisashi
author_sort MIURA, Saori
collection PubMed
description Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomes was detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL) without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to 3 EcoRI and HindIII fragments in these 2 atypical EBL cattle by Southern blotting and hybridization, as well as in 3 typical EBL cattle. The probe also hybridized to a large number of EcoRI and HindIII fragments in 5 cattle with persistent leukosis. These results suggest that the detection of monoclonal integration of BLV provirus into the host genome may serve as a marker of monoclonal proliferation and malignancy in difficult to diagnose EBL cattle.
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spelling pubmed-45275162015-08-07 Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis MIURA, Saori HORIUCHI, Noriyuki MATSUMOTO, Kotaro KOBAYASHI, Yoshiyasu KAWAZU, Shin-ichiro INOKUMA, Hisashi J Vet Med Sci Internal Medicine Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomes was detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL) without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to 3 EcoRI and HindIII fragments in these 2 atypical EBL cattle by Southern blotting and hybridization, as well as in 3 typical EBL cattle. The probe also hybridized to a large number of EcoRI and HindIII fragments in 5 cattle with persistent leukosis. These results suggest that the detection of monoclonal integration of BLV provirus into the host genome may serve as a marker of monoclonal proliferation and malignancy in difficult to diagnose EBL cattle. The Japanese Society of Veterinary Science 2015-03-13 2015-07 /pmc/articles/PMC4527516/ /pubmed/25766769 http://dx.doi.org/10.1292/jvms.14-0591 Text en ©2015 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Internal Medicine
MIURA, Saori
HORIUCHI, Noriyuki
MATSUMOTO, Kotaro
KOBAYASHI, Yoshiyasu
KAWAZU, Shin-ichiro
INOKUMA, Hisashi
Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis
title Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis
title_full Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis
title_fullStr Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis
title_full_unstemmed Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis
title_short Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis
title_sort detection of monoclonal integration of bovine leukemia virus proviral dna as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis
topic Internal Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527516/
https://www.ncbi.nlm.nih.gov/pubmed/25766769
http://dx.doi.org/10.1292/jvms.14-0591
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