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UV-blocking spectacle lens protects against UV-induced decline of visual performance
PURPOSE: Excessive exposure to sunlight may be a risk factor for ocular diseases and reduced visual performance. This study was designed to examine the ability of an ultraviolet (UV)-blocking spectacle lens to prevent visual acuity decline and ocular surface disorders in a mouse model of UVB-induced...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527595/ https://www.ncbi.nlm.nih.gov/pubmed/26283865 |
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author | Liou, Jyh-Cheng Teng, Mei-Ching Tsai, Yun-Shan Lin, En-Chieh Chen, Bo-Yie |
author_facet | Liou, Jyh-Cheng Teng, Mei-Ching Tsai, Yun-Shan Lin, En-Chieh Chen, Bo-Yie |
author_sort | Liou, Jyh-Cheng |
collection | PubMed |
description | PURPOSE: Excessive exposure to sunlight may be a risk factor for ocular diseases and reduced visual performance. This study was designed to examine the ability of an ultraviolet (UV)-blocking spectacle lens to prevent visual acuity decline and ocular surface disorders in a mouse model of UVB-induced photokeratitis. METHODS: Mice were divided into 4 groups (10 mice per group): (1) a blank control group (no exposure to UV radiation), (2) a UVB/no lens group (mice exposed to UVB rays, but without lens protection), (3) a UVB/UV400 group (mice exposed to UVB rays and protected using the CR-39™ spectacle lens [UV400 coating]), and (4) a UVB/photochromic group (mice exposed to UVB rays and protected using the CR-39™ spectacle lens [photochromic coating]). We investigated UVB-induced changes in visual acuity and in corneal smoothness, opacity, and lissamine green staining. We also evaluated the correlation between visual acuity decline and changes to the corneal surface parameters. Tissue sections were prepared and stained immunohistochemically to evaluate the structural integrity of the cornea and conjunctiva. RESULTS: In blank controls, the cornea remained undamaged, whereas in UVB-exposed mice, the corneal surface was disrupted; this disruption significantly correlated with a concomitant decline in visual acuity. Both the UVB/UV400 and UVB/photochromic groups had sharper visual acuity and a healthier corneal surface than the UVB/no lens group. Eyes in both protected groups also showed better corneal and conjunctival structural integrity than unprotected eyes. Furthermore, there were fewer apoptotic cells and less polymorphonuclear leukocyte infiltration in corneas protected by the spectacle lenses. CONCLUSIONS: The model established herein reliably determines the protective effect of UV-blocking ophthalmic biomaterials, because the in vivo protection against UV-induced ocular damage and visual acuity decline was easily defined. |
format | Online Article Text |
id | pubmed-4527595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-45275952015-08-17 UV-blocking spectacle lens protects against UV-induced decline of visual performance Liou, Jyh-Cheng Teng, Mei-Ching Tsai, Yun-Shan Lin, En-Chieh Chen, Bo-Yie Mol Vis Research Article PURPOSE: Excessive exposure to sunlight may be a risk factor for ocular diseases and reduced visual performance. This study was designed to examine the ability of an ultraviolet (UV)-blocking spectacle lens to prevent visual acuity decline and ocular surface disorders in a mouse model of UVB-induced photokeratitis. METHODS: Mice were divided into 4 groups (10 mice per group): (1) a blank control group (no exposure to UV radiation), (2) a UVB/no lens group (mice exposed to UVB rays, but without lens protection), (3) a UVB/UV400 group (mice exposed to UVB rays and protected using the CR-39™ spectacle lens [UV400 coating]), and (4) a UVB/photochromic group (mice exposed to UVB rays and protected using the CR-39™ spectacle lens [photochromic coating]). We investigated UVB-induced changes in visual acuity and in corneal smoothness, opacity, and lissamine green staining. We also evaluated the correlation between visual acuity decline and changes to the corneal surface parameters. Tissue sections were prepared and stained immunohistochemically to evaluate the structural integrity of the cornea and conjunctiva. RESULTS: In blank controls, the cornea remained undamaged, whereas in UVB-exposed mice, the corneal surface was disrupted; this disruption significantly correlated with a concomitant decline in visual acuity. Both the UVB/UV400 and UVB/photochromic groups had sharper visual acuity and a healthier corneal surface than the UVB/no lens group. Eyes in both protected groups also showed better corneal and conjunctival structural integrity than unprotected eyes. Furthermore, there were fewer apoptotic cells and less polymorphonuclear leukocyte infiltration in corneas protected by the spectacle lenses. CONCLUSIONS: The model established herein reliably determines the protective effect of UV-blocking ophthalmic biomaterials, because the in vivo protection against UV-induced ocular damage and visual acuity decline was easily defined. Molecular Vision 2015-08-06 /pmc/articles/PMC4527595/ /pubmed/26283865 Text en Copyright © 2015 Molecular Vision. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed. |
spellingShingle | Research Article Liou, Jyh-Cheng Teng, Mei-Ching Tsai, Yun-Shan Lin, En-Chieh Chen, Bo-Yie UV-blocking spectacle lens protects against UV-induced decline of visual performance |
title | UV-blocking spectacle lens protects against UV-induced decline of visual performance |
title_full | UV-blocking spectacle lens protects against UV-induced decline of visual performance |
title_fullStr | UV-blocking spectacle lens protects against UV-induced decline of visual performance |
title_full_unstemmed | UV-blocking spectacle lens protects against UV-induced decline of visual performance |
title_short | UV-blocking spectacle lens protects against UV-induced decline of visual performance |
title_sort | uv-blocking spectacle lens protects against uv-induced decline of visual performance |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527595/ https://www.ncbi.nlm.nih.gov/pubmed/26283865 |
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