Cargando…

Autologous Graft Thickness Affects Scar Contraction and Quality in a Porcine Excisional Wound Model

BACKGROUND: Texture, color, and durability are important characteristics to consider for skin replacement in conspicuous and/or mobile regions of the body such as the face, neck, and hands. Although autograft thickness is a known determinant of skin quality, few studies have correlated the subjectiv...

Descripción completa

Detalles Bibliográficos
Autores principales: Chan, Rodney K., Rose, Lloyd F., Wu, Jesse C., Tucker, David I., Chan, Maren M., Christy, Robert J., Hale, Robert G., Leung, Kai P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527642/
https://www.ncbi.nlm.nih.gov/pubmed/26301157
http://dx.doi.org/10.1097/GOX.0000000000000426
_version_ 1782384591802204160
author Chan, Rodney K.
Rose, Lloyd F.
Wu, Jesse C.
Tucker, David I.
Chan, Maren M.
Christy, Robert J.
Hale, Robert G.
Leung, Kai P.
author_facet Chan, Rodney K.
Rose, Lloyd F.
Wu, Jesse C.
Tucker, David I.
Chan, Maren M.
Christy, Robert J.
Hale, Robert G.
Leung, Kai P.
author_sort Chan, Rodney K.
collection PubMed
description BACKGROUND: Texture, color, and durability are important characteristics to consider for skin replacement in conspicuous and/or mobile regions of the body such as the face, neck, and hands. Although autograft thickness is a known determinant of skin quality, few studies have correlated the subjective and objective characters of skin graft healing with their associated morphologic and cellular profiles. Defining these relationships may help guide development and evaluation of future skin replacement strategies. METHODS: Six-centimeter-diameter full-thickness wounds were created on the back of female Yorkshire pigs and covered by autografts of variable thicknesses. Skin quality was assessed on day 120 using an observer scar assessment score and objective determinations for scar contraction, erythema, pigmentation, and surface irregularities. Histological, histochemical, and immunohistochemical assessments were performed. RESULTS: Thick grafts demonstrated lower observer scar assessment score (better quality) and decreased erythema, pigmentation, and surface irregularities. Histologically, thin grafts resulted in scar-like collagen proliferation while thick grafts preserves the dermal architecture. Increased vascularity and prolonged and increased cellular infiltration were observed among thin grafts. In addition, thin grafts contained predominately dense collagen fibers, whereas thick grafts had loosely arranged collagen. α-Smooth muscle actin staining for myofibroblasts was observed earlier and persisted longer among thinner grafts. CONCLUSIONS: Graft thickness is an important determinant of skin quality. High-quality skin replacements are associated with preserved collagen architecture, decreased neovascularization, and decreased inflammatory cellular infiltration. This model, using autologous skin as a metric of quality, may give a more informative analysis of emerging skin replacement strategies.
format Online
Article
Text
id pubmed-4527642
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Wolters Kluwer Health
record_format MEDLINE/PubMed
spelling pubmed-45276422015-08-21 Autologous Graft Thickness Affects Scar Contraction and Quality in a Porcine Excisional Wound Model Chan, Rodney K. Rose, Lloyd F. Wu, Jesse C. Tucker, David I. Chan, Maren M. Christy, Robert J. Hale, Robert G. Leung, Kai P. Plast Reconstr Surg Glob Open Experimental BACKGROUND: Texture, color, and durability are important characteristics to consider for skin replacement in conspicuous and/or mobile regions of the body such as the face, neck, and hands. Although autograft thickness is a known determinant of skin quality, few studies have correlated the subjective and objective characters of skin graft healing with their associated morphologic and cellular profiles. Defining these relationships may help guide development and evaluation of future skin replacement strategies. METHODS: Six-centimeter-diameter full-thickness wounds were created on the back of female Yorkshire pigs and covered by autografts of variable thicknesses. Skin quality was assessed on day 120 using an observer scar assessment score and objective determinations for scar contraction, erythema, pigmentation, and surface irregularities. Histological, histochemical, and immunohistochemical assessments were performed. RESULTS: Thick grafts demonstrated lower observer scar assessment score (better quality) and decreased erythema, pigmentation, and surface irregularities. Histologically, thin grafts resulted in scar-like collagen proliferation while thick grafts preserves the dermal architecture. Increased vascularity and prolonged and increased cellular infiltration were observed among thin grafts. In addition, thin grafts contained predominately dense collagen fibers, whereas thick grafts had loosely arranged collagen. α-Smooth muscle actin staining for myofibroblasts was observed earlier and persisted longer among thinner grafts. CONCLUSIONS: Graft thickness is an important determinant of skin quality. High-quality skin replacements are associated with preserved collagen architecture, decreased neovascularization, and decreased inflammatory cellular infiltration. This model, using autologous skin as a metric of quality, may give a more informative analysis of emerging skin replacement strategies. Wolters Kluwer Health 2015-08-10 /pmc/articles/PMC4527642/ /pubmed/26301157 http://dx.doi.org/10.1097/GOX.0000000000000426 Text en Copyright © 2015 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.
spellingShingle Experimental
Chan, Rodney K.
Rose, Lloyd F.
Wu, Jesse C.
Tucker, David I.
Chan, Maren M.
Christy, Robert J.
Hale, Robert G.
Leung, Kai P.
Autologous Graft Thickness Affects Scar Contraction and Quality in a Porcine Excisional Wound Model
title Autologous Graft Thickness Affects Scar Contraction and Quality in a Porcine Excisional Wound Model
title_full Autologous Graft Thickness Affects Scar Contraction and Quality in a Porcine Excisional Wound Model
title_fullStr Autologous Graft Thickness Affects Scar Contraction and Quality in a Porcine Excisional Wound Model
title_full_unstemmed Autologous Graft Thickness Affects Scar Contraction and Quality in a Porcine Excisional Wound Model
title_short Autologous Graft Thickness Affects Scar Contraction and Quality in a Porcine Excisional Wound Model
title_sort autologous graft thickness affects scar contraction and quality in a porcine excisional wound model
topic Experimental
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527642/
https://www.ncbi.nlm.nih.gov/pubmed/26301157
http://dx.doi.org/10.1097/GOX.0000000000000426
work_keys_str_mv AT chanrodneyk autologousgraftthicknessaffectsscarcontractionandqualityinaporcineexcisionalwoundmodel
AT roselloydf autologousgraftthicknessaffectsscarcontractionandqualityinaporcineexcisionalwoundmodel
AT wujessec autologousgraftthicknessaffectsscarcontractionandqualityinaporcineexcisionalwoundmodel
AT tuckerdavidi autologousgraftthicknessaffectsscarcontractionandqualityinaporcineexcisionalwoundmodel
AT chanmarenm autologousgraftthicknessaffectsscarcontractionandqualityinaporcineexcisionalwoundmodel
AT christyrobertj autologousgraftthicknessaffectsscarcontractionandqualityinaporcineexcisionalwoundmodel
AT halerobertg autologousgraftthicknessaffectsscarcontractionandqualityinaporcineexcisionalwoundmodel
AT leungkaip autologousgraftthicknessaffectsscarcontractionandqualityinaporcineexcisionalwoundmodel