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An Exploration of Molecular Correlates Relevant to Radiation Combined Skin-Burn Trauma

BACKGROUND: Exposure to high dose radiation in combination with physical injuries such as burn or wound trauma can produce a more harmful set of medical complications requiring specialist interventions. Currently these interventions are unavailable as are the precise biomarkers needed to help both a...

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Autores principales: Islam, Aminul, Ghimbovschi, Svetlana, Zhai, Min, Swift, Joshua M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527694/
https://www.ncbi.nlm.nih.gov/pubmed/26247844
http://dx.doi.org/10.1371/journal.pone.0134827
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author Islam, Aminul
Ghimbovschi, Svetlana
Zhai, Min
Swift, Joshua M.
author_facet Islam, Aminul
Ghimbovschi, Svetlana
Zhai, Min
Swift, Joshua M.
author_sort Islam, Aminul
collection PubMed
description BACKGROUND: Exposure to high dose radiation in combination with physical injuries such as burn or wound trauma can produce a more harmful set of medical complications requiring specialist interventions. Currently these interventions are unavailable as are the precise biomarkers needed to help both accurately assess and treat such conditions. In the present study, we tried to identify and explore the possible role of serum exosome microRNA (miRNA) signatures as potential biomarkers for radiation combined burn injury (RCBI). METHODOLOGY: Female B6D2F1/J mice were assigned to four experimental groups (n = 6): sham control (SHAM), burn injury (BURN), radiation injury (RI) and combined radiation skin burn injury (CI). We performed serum multiplex cytokine analysis and serum exosome miRNA expression profiling to determine novel miRNA signatures and important biological pathways associated with radiation combined skin-burn trauma. PRINCIPAL FINDINGS: Serum cytokines, IL-5 and MCP-1, were significantly induced only in CI mice (p<0.05). From 890 differentially expressed miRNAs identified, microarray analysis showed 47 distinct miRNA seed sequences significantly associated with CI mice compared to SHAM control mice (fold change ≥ 1.2, p<0.05). Furthermore, only two major miRNA seed sequences (miR-690 and miR-223) were validated to be differentially expressed for CI mice specifically (fold change ≥ 1.5, p<0.05). CONCLUSIONS: Serum exosome miRNA signature data of adult mice, following RCBI, provides new insights into the molecular and biochemical pathways associated with radiation combined skin-burn trauma in vivo.
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spelling pubmed-45276942015-08-12 An Exploration of Molecular Correlates Relevant to Radiation Combined Skin-Burn Trauma Islam, Aminul Ghimbovschi, Svetlana Zhai, Min Swift, Joshua M. PLoS One Research Article BACKGROUND: Exposure to high dose radiation in combination with physical injuries such as burn or wound trauma can produce a more harmful set of medical complications requiring specialist interventions. Currently these interventions are unavailable as are the precise biomarkers needed to help both accurately assess and treat such conditions. In the present study, we tried to identify and explore the possible role of serum exosome microRNA (miRNA) signatures as potential biomarkers for radiation combined burn injury (RCBI). METHODOLOGY: Female B6D2F1/J mice were assigned to four experimental groups (n = 6): sham control (SHAM), burn injury (BURN), radiation injury (RI) and combined radiation skin burn injury (CI). We performed serum multiplex cytokine analysis and serum exosome miRNA expression profiling to determine novel miRNA signatures and important biological pathways associated with radiation combined skin-burn trauma. PRINCIPAL FINDINGS: Serum cytokines, IL-5 and MCP-1, were significantly induced only in CI mice (p<0.05). From 890 differentially expressed miRNAs identified, microarray analysis showed 47 distinct miRNA seed sequences significantly associated with CI mice compared to SHAM control mice (fold change ≥ 1.2, p<0.05). Furthermore, only two major miRNA seed sequences (miR-690 and miR-223) were validated to be differentially expressed for CI mice specifically (fold change ≥ 1.5, p<0.05). CONCLUSIONS: Serum exosome miRNA signature data of adult mice, following RCBI, provides new insights into the molecular and biochemical pathways associated with radiation combined skin-burn trauma in vivo. Public Library of Science 2015-08-06 /pmc/articles/PMC4527694/ /pubmed/26247844 http://dx.doi.org/10.1371/journal.pone.0134827 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Islam, Aminul
Ghimbovschi, Svetlana
Zhai, Min
Swift, Joshua M.
An Exploration of Molecular Correlates Relevant to Radiation Combined Skin-Burn Trauma
title An Exploration of Molecular Correlates Relevant to Radiation Combined Skin-Burn Trauma
title_full An Exploration of Molecular Correlates Relevant to Radiation Combined Skin-Burn Trauma
title_fullStr An Exploration of Molecular Correlates Relevant to Radiation Combined Skin-Burn Trauma
title_full_unstemmed An Exploration of Molecular Correlates Relevant to Radiation Combined Skin-Burn Trauma
title_short An Exploration of Molecular Correlates Relevant to Radiation Combined Skin-Burn Trauma
title_sort exploration of molecular correlates relevant to radiation combined skin-burn trauma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527694/
https://www.ncbi.nlm.nih.gov/pubmed/26247844
http://dx.doi.org/10.1371/journal.pone.0134827
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