Cargando…

Informatics for RNA Sequencing: A Web Resource for Analysis on the Cloud

Massively parallel RNA sequencing (RNA-seq) has rapidly become the assay of choice for interrogating RNA transcript abundance and diversity. This article provides a detailed introduction to fundamental RNA-seq molecular biology and informatics concepts. We make available open-access RNA-seq tutorial...

Descripción completa

Detalles Bibliográficos
Autores principales: Griffith, Malachi, Walker, Jason R., Spies, Nicholas C., Ainscough, Benjamin J., Griffith, Obi L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4527835/
https://www.ncbi.nlm.nih.gov/pubmed/26248053
http://dx.doi.org/10.1371/journal.pcbi.1004393
Descripción
Sumario:Massively parallel RNA sequencing (RNA-seq) has rapidly become the assay of choice for interrogating RNA transcript abundance and diversity. This article provides a detailed introduction to fundamental RNA-seq molecular biology and informatics concepts. We make available open-access RNA-seq tutorials that cover cloud computing, tool installation, relevant file formats, reference genomes, transcriptome annotations, quality-control strategies, expression, differential expression, and alternative splicing analysis methods. These tutorials and additional training resources are accompanied by complete analysis pipelines and test datasets made available without encumbrance at www.rnaseq.wiki.