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Comparative genomic analysis of multiple strains of two unusual plant pathogens: Pseudomonas corrugata and Pseudomonas mediterranea
The non-fluorescent pseudomonads, Pseudomonas corrugata (Pcor) and P. mediterranea (Pmed), are closely related species that cause pith necrosis, a disease of tomato that causes severe crop losses. However, they also show strong antagonistic effects against economically important pathogens, demonstra...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528175/ https://www.ncbi.nlm.nih.gov/pubmed/26300874 http://dx.doi.org/10.3389/fmicb.2015.00811 |
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author | Trantas, Emmanouil A. Licciardello, Grazia Almeida, Nalvo F. Witek, Kamil Strano, Cinzia P. Duxbury, Zane Ververidis, Filippos Goumas, Dimitrios E. Jones, Jonathan D. G. Guttman, David S. Catara, Vittoria Sarris, Panagiotis F. |
author_facet | Trantas, Emmanouil A. Licciardello, Grazia Almeida, Nalvo F. Witek, Kamil Strano, Cinzia P. Duxbury, Zane Ververidis, Filippos Goumas, Dimitrios E. Jones, Jonathan D. G. Guttman, David S. Catara, Vittoria Sarris, Panagiotis F. |
author_sort | Trantas, Emmanouil A. |
collection | PubMed |
description | The non-fluorescent pseudomonads, Pseudomonas corrugata (Pcor) and P. mediterranea (Pmed), are closely related species that cause pith necrosis, a disease of tomato that causes severe crop losses. However, they also show strong antagonistic effects against economically important pathogens, demonstrating their potential for utilization as biological control agents. In addition, their metabolic versatility makes them attractive for the production of commercial biomolecules and bioremediation. An extensive comparative genomics study is required to dissect the mechanisms that Pcor and Pmed employ to cause disease, prevent disease caused by other pathogens, and to mine their genomes for genes that encode proteins involved in commercially important chemical pathways. Here, we present the draft genomes of nine Pcor and Pmed strains from different geographical locations. This analysis covered significant genetic heterogeneity and allowed in-depth genomic comparison. All examined strains were able to trigger symptoms in tomato plants but not all induced a hypersensitive-like response in Nicotiana benthamiana. Genome-mining revealed the absence of type III secretion system and known type III effector-encoding genes from all examined Pcor and Pmed strains. The lack of a type III secretion system appears to be unique among the plant pathogenic pseudomonads. Several gene clusters coding for type VI secretion system were detected in all genomes. Genome-mining also revealed the presence of gene clusters for biosynthesis of siderophores, polyketides, non-ribosomal peptides, and hydrogen cyanide. A highly conserved quorum sensing system was detected in all strains, although species specific differences were observed. Our study provides the basis for in-depth investigations regarding the molecular mechanisms underlying virulence strategies in the battle between plants and microbes. |
format | Online Article Text |
id | pubmed-4528175 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-45281752015-08-21 Comparative genomic analysis of multiple strains of two unusual plant pathogens: Pseudomonas corrugata and Pseudomonas mediterranea Trantas, Emmanouil A. Licciardello, Grazia Almeida, Nalvo F. Witek, Kamil Strano, Cinzia P. Duxbury, Zane Ververidis, Filippos Goumas, Dimitrios E. Jones, Jonathan D. G. Guttman, David S. Catara, Vittoria Sarris, Panagiotis F. Front Microbiol Plant Science The non-fluorescent pseudomonads, Pseudomonas corrugata (Pcor) and P. mediterranea (Pmed), are closely related species that cause pith necrosis, a disease of tomato that causes severe crop losses. However, they also show strong antagonistic effects against economically important pathogens, demonstrating their potential for utilization as biological control agents. In addition, their metabolic versatility makes them attractive for the production of commercial biomolecules and bioremediation. An extensive comparative genomics study is required to dissect the mechanisms that Pcor and Pmed employ to cause disease, prevent disease caused by other pathogens, and to mine their genomes for genes that encode proteins involved in commercially important chemical pathways. Here, we present the draft genomes of nine Pcor and Pmed strains from different geographical locations. This analysis covered significant genetic heterogeneity and allowed in-depth genomic comparison. All examined strains were able to trigger symptoms in tomato plants but not all induced a hypersensitive-like response in Nicotiana benthamiana. Genome-mining revealed the absence of type III secretion system and known type III effector-encoding genes from all examined Pcor and Pmed strains. The lack of a type III secretion system appears to be unique among the plant pathogenic pseudomonads. Several gene clusters coding for type VI secretion system were detected in all genomes. Genome-mining also revealed the presence of gene clusters for biosynthesis of siderophores, polyketides, non-ribosomal peptides, and hydrogen cyanide. A highly conserved quorum sensing system was detected in all strains, although species specific differences were observed. Our study provides the basis for in-depth investigations regarding the molecular mechanisms underlying virulence strategies in the battle between plants and microbes. Frontiers Media S.A. 2015-08-07 /pmc/articles/PMC4528175/ /pubmed/26300874 http://dx.doi.org/10.3389/fmicb.2015.00811 Text en Copyright © 2015 Trantas, Licciardello, Almeida, Witek, Strano, Duxbury, Ververidis, Goumas, Jones, Guttman, Catara and Sarris. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Trantas, Emmanouil A. Licciardello, Grazia Almeida, Nalvo F. Witek, Kamil Strano, Cinzia P. Duxbury, Zane Ververidis, Filippos Goumas, Dimitrios E. Jones, Jonathan D. G. Guttman, David S. Catara, Vittoria Sarris, Panagiotis F. Comparative genomic analysis of multiple strains of two unusual plant pathogens: Pseudomonas corrugata and Pseudomonas mediterranea |
title | Comparative genomic analysis of multiple strains of two unusual plant pathogens: Pseudomonas corrugata and Pseudomonas mediterranea |
title_full | Comparative genomic analysis of multiple strains of two unusual plant pathogens: Pseudomonas corrugata and Pseudomonas mediterranea |
title_fullStr | Comparative genomic analysis of multiple strains of two unusual plant pathogens: Pseudomonas corrugata and Pseudomonas mediterranea |
title_full_unstemmed | Comparative genomic analysis of multiple strains of two unusual plant pathogens: Pseudomonas corrugata and Pseudomonas mediterranea |
title_short | Comparative genomic analysis of multiple strains of two unusual plant pathogens: Pseudomonas corrugata and Pseudomonas mediterranea |
title_sort | comparative genomic analysis of multiple strains of two unusual plant pathogens: pseudomonas corrugata and pseudomonas mediterranea |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528175/ https://www.ncbi.nlm.nih.gov/pubmed/26300874 http://dx.doi.org/10.3389/fmicb.2015.00811 |
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