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An unusual xylan in Arabidopsis primary cell walls is synthesised by GUX3, IRX9L, IRX10L and IRX14

Xylan is a crucial component of many plant primary and secondary cell walls. However, the structure and function of xylan in the dicotyledon primary cell wall is not well understood. Here, we characterized a xylan that is specific to tissues enriched in Arabidopsis primary cell walls. Unlike previou...

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Autores principales: Mortimer, Jenny C, Faria-Blanc, Nuno, Yu, Xiaolan, Tryfona, Theodora, Sorieul, Mathias, Ng, Yao Z, Zhang, Zhinong, Stott, Katherine, Anders, Nadine, Dupree, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528235/
https://www.ncbi.nlm.nih.gov/pubmed/26043357
http://dx.doi.org/10.1111/tpj.12898
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author Mortimer, Jenny C
Faria-Blanc, Nuno
Yu, Xiaolan
Tryfona, Theodora
Sorieul, Mathias
Ng, Yao Z
Zhang, Zhinong
Stott, Katherine
Anders, Nadine
Dupree, Paul
author_facet Mortimer, Jenny C
Faria-Blanc, Nuno
Yu, Xiaolan
Tryfona, Theodora
Sorieul, Mathias
Ng, Yao Z
Zhang, Zhinong
Stott, Katherine
Anders, Nadine
Dupree, Paul
author_sort Mortimer, Jenny C
collection PubMed
description Xylan is a crucial component of many plant primary and secondary cell walls. However, the structure and function of xylan in the dicotyledon primary cell wall is not well understood. Here, we characterized a xylan that is specific to tissues enriched in Arabidopsis primary cell walls. Unlike previously described xylans, this xylan carries a pentose linked 1–2 to the α-1,2-d-glucuronic acid (GlcA) side chains on the β-1,4-Xyl backbone. The frequent and precisely regular spacing of GlcA substitutions every six xylosyl residues along the backbone is also unlike that previously observed in secondary cell wall xylan. Molecular genetics, in vitro assays, and expression data suggest that IRX9L, IRX10L and IRX14 are required for xylan backbone synthesis in primary cell wall synthesising tissues. IRX9 and IRX10 are not involved in the primary cell wall xylan synthesis but are functionally exchangeable with IRX9L and IRX10L. GUX3 is the only glucuronyltransferase required for the addition of the GlcA decorations on the xylan. The differences in xylan structure in primary versus secondary cell walls might reflect the different roles in cross-linking and interaction with other cell wall components.
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spelling pubmed-45282352015-08-13 An unusual xylan in Arabidopsis primary cell walls is synthesised by GUX3, IRX9L, IRX10L and IRX14 Mortimer, Jenny C Faria-Blanc, Nuno Yu, Xiaolan Tryfona, Theodora Sorieul, Mathias Ng, Yao Z Zhang, Zhinong Stott, Katherine Anders, Nadine Dupree, Paul Plant J Original Articles Xylan is a crucial component of many plant primary and secondary cell walls. However, the structure and function of xylan in the dicotyledon primary cell wall is not well understood. Here, we characterized a xylan that is specific to tissues enriched in Arabidopsis primary cell walls. Unlike previously described xylans, this xylan carries a pentose linked 1–2 to the α-1,2-d-glucuronic acid (GlcA) side chains on the β-1,4-Xyl backbone. The frequent and precisely regular spacing of GlcA substitutions every six xylosyl residues along the backbone is also unlike that previously observed in secondary cell wall xylan. Molecular genetics, in vitro assays, and expression data suggest that IRX9L, IRX10L and IRX14 are required for xylan backbone synthesis in primary cell wall synthesising tissues. IRX9 and IRX10 are not involved in the primary cell wall xylan synthesis but are functionally exchangeable with IRX9L and IRX10L. GUX3 is the only glucuronyltransferase required for the addition of the GlcA decorations on the xylan. The differences in xylan structure in primary versus secondary cell walls might reflect the different roles in cross-linking and interaction with other cell wall components. Blackwell Publishing Ltd 2015-08 2015-06-04 /pmc/articles/PMC4528235/ /pubmed/26043357 http://dx.doi.org/10.1111/tpj.12898 Text en © 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Mortimer, Jenny C
Faria-Blanc, Nuno
Yu, Xiaolan
Tryfona, Theodora
Sorieul, Mathias
Ng, Yao Z
Zhang, Zhinong
Stott, Katherine
Anders, Nadine
Dupree, Paul
An unusual xylan in Arabidopsis primary cell walls is synthesised by GUX3, IRX9L, IRX10L and IRX14
title An unusual xylan in Arabidopsis primary cell walls is synthesised by GUX3, IRX9L, IRX10L and IRX14
title_full An unusual xylan in Arabidopsis primary cell walls is synthesised by GUX3, IRX9L, IRX10L and IRX14
title_fullStr An unusual xylan in Arabidopsis primary cell walls is synthesised by GUX3, IRX9L, IRX10L and IRX14
title_full_unstemmed An unusual xylan in Arabidopsis primary cell walls is synthesised by GUX3, IRX9L, IRX10L and IRX14
title_short An unusual xylan in Arabidopsis primary cell walls is synthesised by GUX3, IRX9L, IRX10L and IRX14
title_sort unusual xylan in arabidopsis primary cell walls is synthesised by gux3, irx9l, irx10l and irx14
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528235/
https://www.ncbi.nlm.nih.gov/pubmed/26043357
http://dx.doi.org/10.1111/tpj.12898
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